MicroRNA-18a inhibits ovarian cancer growth via directly targeting TRIAP1 and IPMK

Liu,Ping; Qi,Xiaorong; Bian,Ce; Yang,Fan; Lin,Xiaojuan; Zhou,Shengtao; Xie,Chuan; Zhao,Xia; Yi,Tao

doi:10.3892/ol.2017.5961

MicroRNA-18a inhibits ovarian cancer growth via directly targeting TRIAP1 and IPMK

Authors:
- Ping Liu
- Xiaorong Qi
- Ce Bian
- Fan Yang
- Xiaojuan Lin
- Shengtao Zhou
- Chuan Xie
- Xia Zhao
- Tao Yi
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Affiliations: Department of Gynecology and Obstetrics, Key Laboratory of Birth Defects and Related Diseases of Women and Children of The Ministry of Education, West China Second Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: March 31, 2017 https://doi.org/10.3892/ol.2017.5961
Pages: 4039-4046
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The role of microRNA-18a (miRNA/miR-18a) as a tumor suppressor or promoter in a number of different types of cancer has been reported. However, to date, the expression and the effects of miR‑18a in epithelial ovarian cancer (EOC) remain elusive. In the present study, the expression of miR‑18a in patient EOC tissues and ovarian cancer cell lines was investigated using the reverse transcription‑quantitative polymerase chain reaction. Luciferase assays and western blotting were performed to detect the potential direct targets of miR‑18a. An A2780cp intraperitoneal mouse model, and Cell Counting Kit 8, flow cytometry and terminal deoxynucleotidyl-transferase‑mediated dUTP nick end labeling assays, were used to investigate the effect of miR‑18a on tumor growth in vivo and in vitro. The results indicated that the expression of miR‑18a was reduced in EOC tissue and in the investigated ovarian cancer cell lines compared with non‑malignant (normal) ovarian tissues and the human ovarian epithelium cell line, respectively. Overexpression of miR-18a in the A2780s and A2780cp cell lines significantly induced cell cycle arrest and apoptosis. It was demonstrated that miR‑18a directly targets tumor protein p53‑regulating inhibitor of apoptosis gene 1 and inositol phosphate multikinase, hence regulating the expression of downstream targets. The A2780cp intraperitoneal mouse model was employed and the results indicated that miR‑18a may inhibit A2780cp intraperitoneal tumor growth in vivo by inhibiting proliferation and inducing apoptosis. Together, the results of the present study demonstrated that miR‑18a has a role as a tumor suppressor by inhibiting proliferation and inducing apoptosis. Assessment of miR‑18a expression may provide a novel method for diagnosis and be a therapeutic target for EOC.

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