Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2017 Volume 13 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2017 Volume 13 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism

  • Authors:
    • Tatsuo Kanda
    • Xia Jiang
    • Masato Nakamura
    • Yuki Haga
    • Reina Sasaki
    • Shuang Wu
    • Shingo Nakamoto
    • Fumio Imazeki
    • Osamu Yokosuka
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, Chiba 260‑8670, Japan
  • Pages: 4481-4486
    |
    Published online on: April 3, 2017
       https://doi.org/10.3892/ol.2017.5973
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Hepatocellular carcinoma (HCC) is a predominantly male disease in which the androgen receptor (AR) serves an important pathogenic role in hepatocarcinogenesis. Fatty acid metabolism also contributes to hepatocarcinogenesis and is associated with the prognosis of cancer. The present study aimed to investigate the effects of the AR on fatty acid metabolism‑associated gene expression in human hepatoma cell lines. AR‑expression plasmids or control plasmids were transiently transfected into the human HCC cell lines Huh7 and HepG2. After 48 h, cellular protein and RNA were extracted and the expression of AR was confirmed by western blotting. Complementary DNA was synthesized and subjected to a quantitative polymerase chain reaction‑based array to examine the expression of 84 fatty acid metabolism‑associated genes. Overexpression of AR significantly downregulated the expression of 11 fatty acid metabolism‑associated genes in Huh7 cells and 35 in HepG2 cells. The overexpression of AR also resulted in the upregulation of 6 fatty acid metabolism genes in HepG2 cells; however, it had no effect in Huh7 cells. Acyl‑coenzyme A (CoA) thioesterase 7 and acyl‑CoA oxidase 3 were downregulated in both cell lines. In conclusion, upregulation of AR via overexpression led to the disturbance of fatty acid metabolism-associated gene expression in human HCC cells. Therefore, the AR may serve a role in hepatocarcinogenesis via the regulation of hepatocellular fatty acid metabolism.
View Figures

Figure 1

View References

1 

Nagasue N, Chang YC, Hayashi T, Galizia G, Kohno H, Nakamura T and Yukaya H: Androgen receptor in hepatocellular carcinoma as a prognostic factor after hepatic resection. Ann Surg. 209:424–427. 1989. View Article : Google Scholar : PubMed/NCBI

2 

Okitsu K, Kanda T, Imazeki F, Yonemitsu Y, Ray RB, Chang C and Yokosuka O: Involvement of interleukin-6 and androgen receptor signaling in pancreatic cancer. Genes Cancer. 1:859–867. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Jiang X, Kanda T, Nakamoto S, Miyamura T, Wu S and Yokosuka O: Involvement of androgen receptor and glucose-regulated protein 78 kDa in human hepatocarcinogenesis. Exp Cell Res. 323:326–336. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Rochefort H and Chalbos D: The role of sex steroid receptors on lipogenesis in breast and prostate carcinogenesis: A viewpoint. Horm Cancer. 1:63–70. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Chiu CM, Yeh SH, Chen PJ, Kuo TJ, Chang CJ, Chen PJ, Yang WJ and Chen DS: Hepatitis B virus X protein enhances androgen receptor-responsive gene expression depending on androgen level. Proc Natl Acad Sci USA. 104:2571–2578. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Zheng Y, Chen WL, Ma WL, Chang C and Ou JH: Enhancement of gene transactivation activity of androgen receptor by hepatitis B virus X protein. Virology. 363:454–461. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Kanda T, Steele R, Ray R and Ray RB: Hepatitis C virus core protein augments androgen receptor-mediated signaling. J Virol. 82:11066–11072. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Ueda T, Bruchovsky N and Sadar MD: Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways. J Biol Chem. 277:7076–7085. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Gehlhaus M, Schmitt N, Volk B and Meyer RP: Antiepileptic drugs affect neuronal androgen signaling via a cytochrome P450-dependent pathway. J Pharmacol Exp Ther. 322:550–559. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Yoon G, Kim JY, Choi YK, Won YS and Lim IK: Direct activation of TGF-beta1 transcription by androgen and androgen receptor complex in Huh7 human hepatoma cells and its tumor in nude mice. J Cell Biochem. 97:393–411. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Krycer JR and Brown AJ: Cross-talk between the androgen receptor and the liver X receptor: Implications for cholesterol homeostasis. J Biol Chem. 286:20637–20647. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Hao Q, Li T, Zhang X, Gao P, Qiao P, Li S and Geng Z: Expression and roles of fatty acid synthase in hepatocellular carcinoma. Oncol Rep. 32:2471–2476. 2014.PubMed/NCBI

13 

Qiu JF, Zhang KL, Zhang XJ, Hu YJ, Li P, Shang CZ and Wan JB: Abnormalities in plasma phospholipid fatty acid profiles of patients with hepatocellular carcinoma. Lipids. 50:977–985. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Koh WP, Dan YY, Goh GB, Jin A, Wang R and Yuan JM: Dietary fatty acids and risk of hepatocellular carcinoma in the Singapore Chinese health study. Liver Int. 36:893–901. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Litvinov IV, Chang C and Isaacs JT: Molecular characterization of the commonly used human androgen receptor expression vector, pSG5-AR. Prostate. 58:319–324. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Schröder PC, Segura V, Riezu JI, Sangro B, Mato JM, Prieto J, Santamaría E and Corrales FJ: A signature of six genes highlights defects on cell growth and specific metabolic pathways in murine and human hepatocellular carcinoma. Funct Integr Genomics. 11:419–429. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Suto K, Kajihara-Kano H, Yokoyama Y, Hayakari M, Kimura J, Kumano T, Takahata T, Kudo H and Tsuchida S: Decreased expression of the peroxisomal bifunctional enzyme and carbonyl reductase in human hepatocellular carcinomas. J Cancer Res Clin Oncol. 125:83–88. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Meyer K, Lee JS, Dyck PA, Cao WQ, Rao MS, Thorgeirsson SS and Reddy JK: Molecular profiling of hepatocellular carcinomas developing spontaneously in acyl-CoA oxidase deficient mice: Comparison with liver tumors induced in wild-type mice by a peroxisome proliferator and a genotoxic carcinogen. Carcinogenesis. 24:975–984. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Li J, Huang Q, Long X, Zhang J, Huang X, Aa J, Yang H, Chen Z and Xing J: CD147 reprograms fatty acid metabolism in hepatocellular carcinoma cells through Akt/mTOR/SREBP1c and P38/PPARα pathways. J Hepatol. 63:1378–1389. 2015. View Article : Google Scholar : PubMed/NCBI

21 

Muir K, Hazim A, He Y, Peyressatre M, Kim DY, Song X and Beretta L: Proteomic and lipidomic signatures of lipid metabolism in NASH-associated hepatocellular carcinoma. Cancer Res. 73:4722–4731. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Jin S, Chen J, Chen L, Histen G, Lin Z, Gross S, Hixon J, Chen Y, Kung C, Chen Y, et al: ALDH2(E487K) mutation increases protein turnover and promotes murine hepatocarcinogenesis. Proc Natl Acad Sci USA. 112:9088–9093. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Honeyman JN, Simon EP, Robine N, Chiaroni-Clarke R, Darcy DG, Lim II, Gleason CE, Murphy JM, Rosenberg BR, Teegan L, et al: Detection of a recurrent DNAJB1-PRKACA chimeric transcript in fibrolamellar hepatocellular carcinoma. Science. 343:1010–1014. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Sakabe T, Tsuchiya H, Kanki K, Azumi J, Gonda K, Mizuta Y, Yamada D, Wada H, Shomori K, Nagano H and Shiota G: Identification of the genes chemosensitizing hepatocellular carcinoma cells to interferon-α/5-fluorouracil and their clinical significance. PLoS One. 8:e561972013. View Article : Google Scholar : PubMed/NCBI

25 

Ellis JM, Wong GW and Wolfgang MJ: Acyl coenzyme A thioesterase 7 regulates neuronal fatty acid metabolism to prevent neurotoxicity. Mol Cell Biol. 33:1869–1882. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Zha S, Ferdinandusse S, Hicks JL, Denis S, Dunn TA, Wanders RJ, Luo J, De Marzo AM and Isaacs WB: Peroxisomal branched chain fatty acid beta-oxidation pathway is upregulated in prostate cancer. Prostate. 63:316–323. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Bolton EC, So AY, Chaivorapol C, Haqq CM, Li H and Yamamoto KR: Cell- and gene-specific regulation of primary target genes by the androgen receptor. Genes Dev. 21:2005–2017. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Baron A, Migita T, Tang D and Loda M: Fatty acid synthase: A metabolic oncogene in prostate cancer? J Cell Biochem. 91:47–53. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Mounier C, Bouraoui L and Rassart E: Lipogenesis in cancer progression (review). Int J Oncol. 45:485–492. 2014.PubMed/NCBI

30 

Jiang J, Nilsson-Ehle P and Xu N: Influence of liver cancer on lipid and lipoprotein metabolism. Lipids Health Dis. 5:42006. View Article : Google Scholar : PubMed/NCBI

31 

Miyanari Y, Atsuzawa K, Usuda N, Watashi K, Hishiki T, Zayas M, Bartenschlager R, Wakita T, Hijikata M and Shimotohno K: The lipid droplet is an important organelle for hepatitis C virus production. Nat Cell Biol. 9:1089–1097. 2007. View Article : Google Scholar : PubMed/NCBI

32 

Meissner EG, Lee YJ, Osinusi A, Sims Z, Qin J, Sturdevant D, McHutchison J, Subramanian M, Sampson M, Naggie S, et al: Effect of sofosbuvir and ribavirin treatment on peripheral and hepatic lipid metabolism in chronic hepatitis C virus, genotype 1-infected patients. Hepatology. 61:790–801. 2015. View Article : Google Scholar : PubMed/NCBI

33 

Wu YL, Peng XE, Zhu YB, Yan XL, Chen WN and Lin X: Hepatitis B virus X protein induces hepatic steatosis by enhancing the expression of liver fatty acid binding protein. J Virol. 90:1729–1740. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Haga Y, Kanda T, Sasaki R, Nakamura M, Nakamoto S and Yokosuka O: Nonalcoholic fatty liver disease and hepatic cirrhosis: Comparison with viral hepatitis-associated steatosis. World J Gastroenterol. 21:12989–12995. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kanda T, Jiang X, Nakamura M, Haga Y, Sasaki R, Wu S, Nakamoto S, Imazeki F and Yokosuka O: Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism. Oncol Lett 13: 4481-4486, 2017.
APA
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S. ... Yokosuka, O. (2017). Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism. Oncology Letters, 13, 4481-4486. https://doi.org/10.3892/ol.2017.5973
MLA
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S., Nakamoto, S., Imazeki, F., Yokosuka, O."Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism". Oncology Letters 13.6 (2017): 4481-4486.
Chicago
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S., Nakamoto, S., Imazeki, F., Yokosuka, O."Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism". Oncology Letters 13, no. 6 (2017): 4481-4486. https://doi.org/10.3892/ol.2017.5973
Copy and paste a formatted citation
x
Spandidos Publications style
Kanda T, Jiang X, Nakamura M, Haga Y, Sasaki R, Wu S, Nakamoto S, Imazeki F and Yokosuka O: Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism. Oncol Lett 13: 4481-4486, 2017.
APA
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S. ... Yokosuka, O. (2017). Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism. Oncology Letters, 13, 4481-4486. https://doi.org/10.3892/ol.2017.5973
MLA
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S., Nakamoto, S., Imazeki, F., Yokosuka, O."Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism". Oncology Letters 13.6 (2017): 4481-4486.
Chicago
Kanda, T., Jiang, X., Nakamura, M., Haga, Y., Sasaki, R., Wu, S., Nakamoto, S., Imazeki, F., Yokosuka, O."Overexpression of the androgen receptor in human hepatoma cells and its effect on fatty acid metabolism". Oncology Letters 13, no. 6 (2017): 4481-4486. https://doi.org/10.3892/ol.2017.5973
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team