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A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro

  • Authors:
    • Zhen Liang
    • Chun‑Sheng Yang
    • Feng Gu
    • Lan‑Sheng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Xuzhou Central Hospital, Xuzhou, Jiangsu 221009, P.R. China, Department of Dermatology, Affiliated Huai'an Hospital of Xuzhou Medical University, Huaian, Jiangsu 223002, P.R. China, Clinical Laboratory, Xuzhou Tumor Hospital, Xuzhou, Jiangsu 221002, P.R. China, Department of Oncology, The Second Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4185-4189
    |
    Published online on: April 7, 2017
       https://doi.org/10.3892/ol.2017.6007
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Abstract

Malignant melanoma is characterized by suppressed apoptosis in tumor cells and high levels of invasion. Temozolomide (TMZ) is one of the most effective single chemotherapeutic agents for patients with malignant melanoma, but resistance develops quickly and frequently. Therapeutic cytokines such as interleukin‑24 (IL‑24) and conditionally replicating adenoviruses have exhibited promising results as complementary therapies. Thus, the present study hypothesized that a conditionally replicating adenovirus expressing IL‑24 combined with TMZ may exhibit increased antitumor activity compared with either treatment alone in melanoma A375 and M14 cell lines in vitro. The present study constructed an E1B‑55 gene‑deleted conditionally replicating adenovirus expressing the IL‑24 gene (ZD55‑IL‑24). IL‑24 was expressed at high levels in melanoma cells infected with ZD55‑IL‑24 in the presence of TMZ. The combination of ZD55‑IL‑24 + TMZ induced higher protein expression levels of the proapoptotic proteins B‑cell lymphoma‑2 (Bcl‑2)‑like protein 4 and phosphorylated protein, γ‑H2A histone family member X (γ‑H2AX), and reduced the levels of the antiapoptotic proteins Bcl‑2, myeloid cell leukemia‑1and nuclear factor‑κB compared with either treatment individually. A dose‑dependent increase in the cytopathic effects for the combination of ZD55‑IL‑24 and TMZ was also observed. The data of the present study suggest that the ZD55‑IL‑24 + TMZ combination induced increased levels of apoptosis, possibly by triggering DNA damage, in melanoma cells in vitro compared with either treatment alone. These findings suggest that this strategy may be a promising approach for the treatment of patients with malignant melanoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liang Z, Yang CS, Gu F and Zhang LS: A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro. Oncol Lett 13: 4185-4189, 2017.
APA
Liang, Z., Yang, C., Gu, F., & Zhang, L. (2017). A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro. Oncology Letters, 13, 4185-4189. https://doi.org/10.3892/ol.2017.6007
MLA
Liang, Z., Yang, C., Gu, F., Zhang, L."A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro". Oncology Letters 13.6 (2017): 4185-4189.
Chicago
Liang, Z., Yang, C., Gu, F., Zhang, L."A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro". Oncology Letters 13, no. 6 (2017): 4185-4189. https://doi.org/10.3892/ol.2017.6007
Copy and paste a formatted citation
x
Spandidos Publications style
Liang Z, Yang CS, Gu F and Zhang LS: A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro. Oncol Lett 13: 4185-4189, 2017.
APA
Liang, Z., Yang, C., Gu, F., & Zhang, L. (2017). A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro. Oncology Letters, 13, 4185-4189. https://doi.org/10.3892/ol.2017.6007
MLA
Liang, Z., Yang, C., Gu, F., Zhang, L."A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro". Oncology Letters 13.6 (2017): 4185-4189.
Chicago
Liang, Z., Yang, C., Gu, F., Zhang, L."A conditionally replicating adenovirus expressing IL-24 acts synergistically with temozolomide to enhance apoptosis in melanoma cells in vitro". Oncology Letters 13, no. 6 (2017): 4185-4189. https://doi.org/10.3892/ol.2017.6007
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