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Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer

  • Authors:
    • Eunjoo Hwang
    • Ji‑Hyun Uh
    • Hye Seon Lee
    • Cham Han Lee
    • Soo Jeong Lee
    • Sei Hyun Ahn
    • Byung Ho Son
    • Jong Won Lee
    • Jong Han Yu
    • Nak‑Jung Kwon
    • Woo Chung Lee
    • Kap‑Seok Yang
    • Sung Ho Choi
    • Myoung Shin Kim
    • Jinseon Lee
    • Byung Hee Jeon
  • View Affiliations / Copyright

    Affiliations: Cytogen Incorporated, Songpa, Seoul 138‑961, Republic of Korea, Department of Surgery, Asan Medical Center, College of Medicine, University of Ulsan, Songpa, Seoul 138‑736, Republic of Korea, Macrogen Incorporated, Geumcheon, Seoul 153‑781, Republic of Korea
    Copyright: © Hwang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4627-4632
    |
    Published online on: April 24, 2017
       https://doi.org/10.3892/ol.2017.6077
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Abstract

Although numerous effective therapies have improved the survival rate of patients with breast cancer, a number of patients present with tumor recurrence and metastasis. A liquid biopsy of circulating tumor cells (CTC) is a non‑invasive method to obtain tumor cells and may be used as substitute for a tumor tissue biopsy. The present study focuses on determining whether CTC culture is an optimal method of obtaining sufficient amounts of CTCs for molecular analysis. The current study demonstrates a method of isolating and culturing CTCs from patients with breast cancer and the construction of a molecular profile of cultured cells using the Ion AmpliSeq Cancer Gene Panel V2. Gene mutations that were observed in cultured CTCs were compared with those observed in primary tumor tissues. CTCs were isolated and cultured from the blood of six patients with breast cancer. Mutations from the Catalogue Of Somatic Mutation In Cancer (COSMIC) were detected in Platelet‑Derived Growth Factor Receptor Alpha, MET (also known as Hepatocyte Growth Factor Receptor), Phosphatase and Tensin Homolog, Harvey Rat Sarcoma Viral Oncogene Homolog, SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin Subfamily B Member 1, Cyclin Dependent Kinase Inhibitor 2A and MutL Homolog 1 genes in 5/6 samples. A comparison between mutations detected in cultured CTCs and mutations detected in primary tumor tissues demonstrated that a large number of mutations that were identified in CTCs were also detected in primary tumor tissues. The results from the current study describe a novel cell culture approach that may be used to obtain an optimal number of CTCs for molecular analysis. This novel approach is able to be used as a tool for liquid biopsy during breast cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Hwang E, Uh JH, Lee HS, Lee CH, Lee SJ, Ahn SH, Son BH, Lee JW, Yu JH, Kwon NJ, Kwon NJ, et al: Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer. Oncol Lett 13: 4627-4632, 2017.
APA
Hwang, E., Uh, J., Lee, H.S., Lee, C.H., Lee, S.J., Ahn, S.H. ... Jeon, B.H. (2017). Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer. Oncology Letters, 13, 4627-4632. https://doi.org/10.3892/ol.2017.6077
MLA
Hwang, E., Uh, J., Lee, H. S., Lee, C. H., Lee, S. J., Ahn, S. H., Son, B. H., Lee, J. W., Yu, J. H., Kwon, N., Lee, W. C., Yang, K., Choi, S. H., Kim, M. S., Lee, J., Jeon, B. H."Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer". Oncology Letters 13.6 (2017): 4627-4632.
Chicago
Hwang, E., Uh, J., Lee, H. S., Lee, C. H., Lee, S. J., Ahn, S. H., Son, B. H., Lee, J. W., Yu, J. H., Kwon, N., Lee, W. C., Yang, K., Choi, S. H., Kim, M. S., Lee, J., Jeon, B. H."Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer". Oncology Letters 13, no. 6 (2017): 4627-4632. https://doi.org/10.3892/ol.2017.6077
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang E, Uh JH, Lee HS, Lee CH, Lee SJ, Ahn SH, Son BH, Lee JW, Yu JH, Kwon NJ, Kwon NJ, et al: Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer. Oncol Lett 13: 4627-4632, 2017.
APA
Hwang, E., Uh, J., Lee, H.S., Lee, C.H., Lee, S.J., Ahn, S.H. ... Jeon, B.H. (2017). Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer. Oncology Letters, 13, 4627-4632. https://doi.org/10.3892/ol.2017.6077
MLA
Hwang, E., Uh, J., Lee, H. S., Lee, C. H., Lee, S. J., Ahn, S. H., Son, B. H., Lee, J. W., Yu, J. H., Kwon, N., Lee, W. C., Yang, K., Choi, S. H., Kim, M. S., Lee, J., Jeon, B. H."Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer". Oncology Letters 13.6 (2017): 4627-4632.
Chicago
Hwang, E., Uh, J., Lee, H. S., Lee, C. H., Lee, S. J., Ahn, S. H., Son, B. H., Lee, J. W., Yu, J. H., Kwon, N., Lee, W. C., Yang, K., Choi, S. H., Kim, M. S., Lee, J., Jeon, B. H."Cancer gene panel analysis of cultured circulating tumor cells and primary tumor tissue from patients with breast cancer". Oncology Letters 13, no. 6 (2017): 4627-4632. https://doi.org/10.3892/ol.2017.6077
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