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Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1

  • Authors:
    • Jing‑Hao Wang
    • Xiu‑Wen Wang
    • Di Qu
    • Ji‑Wen Sun
    • Fei‑Xiao Guo
    • Dan Lu
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4695-4700
    |
    Published online on: April 24, 2017
       https://doi.org/10.3892/ol.2017.6078
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Abstract

Cytokine-induced apoptosis inhibitor 1 (CIAPIN1), originally termed anamorsin, is an anti‑apoptotic molecule that acts as a downstream effector of the receptor tyrosine kinase‑Ras signaling pathway. Overexpression of CIAPIN1 contributes to multidrug resistance (MDR) and microRNA (miR)‑143 is typically considered a tumor suppressor in breast cancer. The present study aimed to evaluate the therapeutic potential of miR‑143 as a treatment for drug‑resistant breast cancer via the downregulation of CIAPIN1 in vitro. The expression levels of miR‑143 were measured using quantitative polymerase chain reaction and the expression levels of CIAPIN1 were detected via western blot analysis. Bioinformatic analyses was additionally conducted to search for miR‑143, which may potentially target CIAPIN1. Luciferase reporter plasmids were created and used to verify direct targeting. In addition, Taxol‑induced drug‑resistant (TDR) breast cancer cell proliferation was evaluated using the Cell Counting Kit‑8 assay in vitro. The present study identified an inverse association between miR‑143 and CIAPIN1 protein expression levels in breast cancer MCF‑7, MDA‑MB‑231 and MDA‑MB‑453 TDR cells. Specific targeting sites for miR‑143 in the 3'‑untranslated region of the CIAPIN1 gene were identified, which exhibit the ability to regulate CIAPIN1 expression. It was revealed that the repression of CIAPIN1 via miR‑143 suppressed the proliferation of breast cancer TDR cells. The findings of the present study verified the role of miR‑143 as a tumor suppressor in breast cancer MDR via inhibition of CIAPIN1 translation.
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Copy and paste a formatted citation
Spandidos Publications style
Wang JH, Wang XW, Qu D, Sun JW, Guo FX and Lu D: Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncol Lett 13: 4695-4700, 2017.
APA
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., & Lu, D. (2017). Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncology Letters, 13, 4695-4700. https://doi.org/10.3892/ol.2017.6078
MLA
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., Lu, D."Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1". Oncology Letters 13.6 (2017): 4695-4700.
Chicago
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., Lu, D."Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1". Oncology Letters 13, no. 6 (2017): 4695-4700. https://doi.org/10.3892/ol.2017.6078
Copy and paste a formatted citation
x
Spandidos Publications style
Wang JH, Wang XW, Qu D, Sun JW, Guo FX and Lu D: Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncol Lett 13: 4695-4700, 2017.
APA
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., & Lu, D. (2017). Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1. Oncology Letters, 13, 4695-4700. https://doi.org/10.3892/ol.2017.6078
MLA
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., Lu, D."Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1". Oncology Letters 13.6 (2017): 4695-4700.
Chicago
Wang, J., Wang, X., Qu, D., Sun, J., Guo, F., Lu, D."Upregulation of microRNA-143 reverses drug resistance in human breast cancer cells via inhibition of cytokine-induced apoptosis inhibitor 1". Oncology Letters 13, no. 6 (2017): 4695-4700. https://doi.org/10.3892/ol.2017.6078
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