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Article Open Access

Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells

  • Authors:
    • Yingchun Qu
    • Peifang Cong
    • Chengjiang Lin
    • Yihui Deng
    • Jesse Li‑Ling
    • Meixia Zhang
  • View Affiliations / Copyright

    Affiliations: Institute of Metabolic Disease Research and Drug Development, China Medical University, Shenyang, Liaoning 110122, P.R. China, Department of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning 110015, P.R. China, Nanchuan Institute of Biological Research, Joint Key Laboratory for Bioresource Research and Utilization of Sichuan and Chongqing, Chongqing 408400, P.R. China
    Copyright: © Qu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 145-152
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    Published online on: May 10, 2017
       https://doi.org/10.3892/ol.2017.6148
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Abstract

Ovarian cancer is the leading cause of mortality among all gynecological malignancies. Drug resistance is a cause of ovarian cancer recurrence and low rate of overall survival. There is a requirement for more effective treatment approaches. Cucurbitacin B (CuB) is an antineoplastic agent derived from traditional Chinese medicinal herbs. Its activity against paclitaxel‑resistant human ovarian cancer cells has, however, not yet been established. The purpose of the present study was to investigate the effect and mechanism of CuB on human paclitaxel‑resistant ovarian cancer A2780/Taxol cells. Cell viability was evaluated by a cell counting assay, while cell cycle arrest and apoptosis were assessed by microscopy and flow cytometry, and proteins associated with apoptotic pathways and drug resistance were evaluated by western blotting. The present results demonstrated that CuB exerts dose‑ and time‑dependent cytotoxicity against the ovarian cancer A2780 cell line, with half‑maximal inhibitory concentration (IC50) values 0.48, 0.25 and 0.21 µM following 24, 48 and 72 h of incubation, respectively. Compared with its sensitive counterpart, A2780, paclitaxel‑resistant A2780/Taxol cells had almost identical IC50 values. Cell cycle analysis demonstrated that treatment with CuB may induce cell cycle arrest at the G2/M phase of the cell cycle in the two cell lines. As revealed by Annexin V/propidium iodide‑labeled flow cytometry and Hoechst 33258 staining, CuB‑induced apoptosis was accompanied by activation of caspase‑3 and downregulation of B‑cell lymphoma‑2. Western blotting demonstrated that CuB may enhance the expression of p53 and p21 in the two cell lines. CuB may also downregulate the expression of P‑glycoprotein. These results indicate that CuB may exert a therapeutic effect on paclitaxel‑resistant human ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Qu Y, Cong P, Lin C, Deng Y, Li‑Ling J and Zhang M: Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells. Oncol Lett 14: 145-152, 2017.
APA
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., & Zhang, M. (2017). Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells. Oncology Letters, 14, 145-152. https://doi.org/10.3892/ol.2017.6148
MLA
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., Zhang, M."Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells". Oncology Letters 14.1 (2017): 145-152.
Chicago
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., Zhang, M."Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells". Oncology Letters 14, no. 1 (2017): 145-152. https://doi.org/10.3892/ol.2017.6148
Copy and paste a formatted citation
x
Spandidos Publications style
Qu Y, Cong P, Lin C, Deng Y, Li‑Ling J and Zhang M: Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells. Oncol Lett 14: 145-152, 2017.
APA
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., & Zhang, M. (2017). Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells. Oncology Letters, 14, 145-152. https://doi.org/10.3892/ol.2017.6148
MLA
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., Zhang, M."Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells". Oncology Letters 14.1 (2017): 145-152.
Chicago
Qu, Y., Cong, P., Lin, C., Deng, Y., Li‑Ling, J., Zhang, M."Inhibition of paclitaxel resistance and apoptosis induction by cucurbitacin B in ovarian carcinoma cells". Oncology Letters 14, no. 1 (2017): 145-152. https://doi.org/10.3892/ol.2017.6148
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