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Article Open Access

S100P is associated with proliferation and migration in nasopharyngeal carcinoma

  • Authors:
    • Yueyang Liu
    • Chengyu Wang
    • Xiaodong Shan
    • Jian Wu
    • Huanhai Liu
    • Haibin Liu
    • Jiping Zhang
    • Weihua Xu
    • Zhirong Sha
    • Jin He
    • Jingping Fan
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Gongli Hospital, Shanghai 200135, P.R. China, Department of Otolaryngology‑Head and Neck Surgery, Jiangsu Qidong Traditional Chinese Medical Hospital, Qidong, Jiangsu 226200, P.R. China, Department of Pathology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 525-532
    |
    Published online on: May 17, 2017
       https://doi.org/10.3892/ol.2017.6198
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Abstract

In the present study, the function of S100 calcium binding protein P (S100P) in the C666‑1 nasopharyngeal carcinoma (NPC) cell line was examined. The levels of S100P protein in NPC tissues were analyzed using immunohistochemistry, and small interfering RNA silenced S100P expression in C666‑1 cells. Subsequently, cell proliferation, colony formation, migration and wound-healing assays were performed in order to assess whether the knockdown of S100P was able to influence the biological behavior of C666‑1 cells. The expression levels of the receptor for advanced glycation end products (RAGE) were analyzed using a western blot following the inhibition of S100P. The immunohistochemistry results revealed that S100P was elevated in expression in 45/78 (57.7%) of patients with NPC, as compared with 5/30 (16.7%) of patients with benign inflammation. The S100P protein levels correlated with the rates of proliferation and migration in C666‑1 cells. Additionally, reduced S100P expression levels altered a series of intracellular events, including the downregulation of epidermal growth factor receptor, cluster of differentiation (CD) 44, matrix metalloproteinase (MMP) 2 and MMP9 protein expression. In addition, RAGE expression was downregulated in the S100P silenced C666‑1 cells, as detected by western blot analysis. These data suggest that S100P is important during the development and progression of nasopharyngeal cancer. Therefore, S100P may provide a novel treatment target for NPC.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Wang C, Shan X, Wu J, Liu H, Liu H, Zhang J, Xu W, Sha Z, He J, He J, et al: S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14: 525-532, 2017.
APA
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H. ... Fan, J. (2017). S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncology Letters, 14, 525-532. https://doi.org/10.3892/ol.2017.6198
MLA
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H., Zhang, J., Xu, W., Sha, Z., He, J., Fan, J."S100P is associated with proliferation and migration in nasopharyngeal carcinoma". Oncology Letters 14.1 (2017): 525-532.
Chicago
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H., Zhang, J., Xu, W., Sha, Z., He, J., Fan, J."S100P is associated with proliferation and migration in nasopharyngeal carcinoma". Oncology Letters 14, no. 1 (2017): 525-532. https://doi.org/10.3892/ol.2017.6198
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Wang C, Shan X, Wu J, Liu H, Liu H, Zhang J, Xu W, Sha Z, He J, He J, et al: S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncol Lett 14: 525-532, 2017.
APA
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H. ... Fan, J. (2017). S100P is associated with proliferation and migration in nasopharyngeal carcinoma. Oncology Letters, 14, 525-532. https://doi.org/10.3892/ol.2017.6198
MLA
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H., Zhang, J., Xu, W., Sha, Z., He, J., Fan, J."S100P is associated with proliferation and migration in nasopharyngeal carcinoma". Oncology Letters 14.1 (2017): 525-532.
Chicago
Liu, Y., Wang, C., Shan, X., Wu, J., Liu, H., Liu, H., Zhang, J., Xu, W., Sha, Z., He, J., Fan, J."S100P is associated with proliferation and migration in nasopharyngeal carcinoma". Oncology Letters 14, no. 1 (2017): 525-532. https://doi.org/10.3892/ol.2017.6198
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