Improved antitumor effect of ionizing radiation in combination with rapamycin for treating nasopharyngeal carcinoma

  • Authors:
    • Di Wang
    • Lichen Gao
    • Xueting Liu
    • Chuang Yuan
    • Guihua Wang
  • View Affiliations

  • Published online on: May 19, 2017     https://doi.org/10.3892/ol.2017.6208
  • Pages: 1105-1108
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The aim of the present study is to investigate if rapamycin is a radiosensitizer of nasopharyngeal carcinoma (NPC), and to identify which pathways are involved in radiation sensitization. In vitro, using untreated cells as the control, NPC cells were treated with rapamycin, ionizing radiation (IR) or both. Differences in the phosphorylation of ribosomal protein S6 and glycogen synthase kinase (GSK) 3β, expression of cyclin D1, clonogenic survival, number of phosphorylated histone subunit 2AX (γH2AX) foci, and cell cycle status between the study groups were compared. The results indicated that rapamycin alone decreased the phosphorylation of S6 and GSK3β, as well as the expression of cyclin D1, in NPC cells. Thus, rapamycin‑treated NPC cells had lower cell viability, and higher DNA damage and G1 arrest than control cells. In addition, the combination of rapamycin and IR caused the highest cell death, DNA damage and G1 arrest when compared with the effects caused by either treatment alone. In conclusion, rapamycin improves the anti‑tumor effect of IR for treating NPC through inhibiting the Akt/mechanistic target of rapamycin/S6 and Akt/GSK3β/cyclin D1 signaling pathways.
View Figures
View References

Related Articles

Journal Cover

July-2017
Volume 14 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Gao L, Liu X, Yuan C and Wang G: Improved antitumor effect of ionizing radiation in combination with rapamycin for treating nasopharyngeal carcinoma. Oncol Lett 14: 1105-1108, 2017.
APA
Wang, D., Gao, L., Liu, X., Yuan, C., & Wang, G. (2017). Improved antitumor effect of ionizing radiation in combination with rapamycin for treating nasopharyngeal carcinoma. Oncology Letters, 14, 1105-1108. https://doi.org/10.3892/ol.2017.6208
MLA
Wang, D., Gao, L., Liu, X., Yuan, C., Wang, G."Improved antitumor effect of ionizing radiation in combination with rapamycin for treating nasopharyngeal carcinoma". Oncology Letters 14.1 (2017): 1105-1108.
Chicago
Wang, D., Gao, L., Liu, X., Yuan, C., Wang, G."Improved antitumor effect of ionizing radiation in combination with rapamycin for treating nasopharyngeal carcinoma". Oncology Letters 14, no. 1 (2017): 1105-1108. https://doi.org/10.3892/ol.2017.6208