Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2017 Volume 14 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes

  • Authors:
    • Fei Guo
    • Junjie Zhang
    • Lei Wang
    • Wei Zhao
    • Jiekai Yu
    • Shu Zheng
    • Jiaxiang Wang
  • View Affiliations / Copyright

    Affiliations: Department of Pediatric Surgery, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Institute of Cancer, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 687-694
    |
    Published online on: May 26, 2017
       https://doi.org/10.3892/ol.2017.6261
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study aimed to identify differentially expressed inflammatory factors observed in Wilms' tumors (WT), and to investigate the association of these factors with clinical stage, pathological type, lymph node metastasis and vascular involvement of WT. Surface‑enhanced laser desorption/ionization‑time of flight mass spectrometry was performed to screen differentially expressed proteins among WT and normal tissue pairs. Upregulated proteins in WT were separated and purified by solid phase extraction and Tricine SDS‑PAGE, respectively. Following in‑gel digestion, the peptide mixture was subjected to liquid chromatography mass spectrometry to identify proteins on the basis of their amino acid sequences. Immunohistochemistry was used to confirm the expression of differentially expressed inflammatory proteins. Of the proteins that were upregulated in WT, two proteins with mass/charge (m/z) ratio of 12,138 and 13,462 were identified as macrophage migration inhibitory factor (MIF) and C‑X‑C motif ligand 7 (CXCL7) chemokine, respectively. The expression of these two proteins was increased in WT compared with adjacent normal tissues and normal renal tissues, and increased with increasing clinical stage. In addition, their expression was significantly increased in patients with unfavorable pathological type, lymph node metastasis and vascular involvement compared with the groups with favorable type, and without lymph node metastasis or vascular involvement (P<0.05). Increased pro‑inflammatory MIF and CXCL7 expression in WT is closely associated with the clinical stage, pathological type, lymph node metastasis and vascular involvement, and may represent biomarkers for the clinical diagnosis of WT.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Metzger ML and Dome JS: Current therapy for Wilms tumor. Oncologist. 10:815–826. 2005. View Article : Google Scholar : PubMed/NCBI

2 

Fan Y, Shi L, Liu Q, Dong R, Zhang Q, Yang S, Fan Y, Yang H, Wu P, Yu J, et al: Discovery and identification of potential biomarkers of papillary thyroid carcinoma. Molecular Cancer. 8:792009. View Article : Google Scholar : PubMed/NCBI

3 

Zhang Q, Wang J, Dong R, Yang S and Zheng S: Identification of novel serum biomarkers in child nephroblastoma using proteomics technology. Mol Biol Rep. 38:631–638. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Fan Y and Wang J, Yang Y, Liu Q, Fan Y, Yu J, Zheng S, Li M and Wang J: Detection and identification of potential biomarkers of breast cancer. J Cancer Res Clin. 136:1243–1254. 2010. View Article : Google Scholar

5 

Mitchell JK, Lemon SM and McGivern DR: How do persistent infections with hepatitis C virus cause liver cancer? Curr Opin Virol. 14:101–108. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Umansky V and Sevko A: Overcoming immunosuppression in the melanoma microenvironment induced by chronic inflammation. Cancer Immunol Immunother. 61:275–282. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Kanterman J, Sade-Feldman M and Baniyash M: New insights into chronic inflammation-induced immunosuppression. Semin Cancer Biol. 22:307–318. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Ringelhan M, OConnor T, Protzer U and Heikenwalder M: The direct and indirect roles of HBV in liver cancer: Prospective markers for HCC screening and potential therapeutic targets. J Pathol. 235:355–367. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Lue H, Kleemann R, Calandra T, Roger T and Bernhagen J: Macrophage migration inhibitory factor (MIF): Mechanisms of action and role in disease. Microbes Infect. 4:449–460. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Calandra T and Roger T: Macrophage migration inhibitory factor: A regulator of innate immunity. Nat Rev Immunol. 3:791–800. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Grieb G, Merk M, Bernhagen J and Bucala R: Macrophage migration inhibitory factor (MIF): A promising biomarker. Drug News Perspect. 23:257–264. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Meyer-Siegler KL, Iczkowski KA, Leng L, Bucala R and Vera PL: Inhibition of macrophage migration inhibitory factor or its receptor (CD74) attenuates growth and invasion of DU-145 prostate cancer cells. J Immunol. 177:8730–8739. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Choudhary S, Hegde P, Pruitt JR, Sielecki TM, Choudhary D, Scarpato K, Degraff DJ, Pilbeam CC and Taylor JA III: Macrophage migratory inhibitory factor promotes bladder cancer progression via increasing proliferation and angiogenesis. Carcinogenesis. 34:2891–2899. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Arenberg D, Luckhardt TR, Carskadon S, Zhao L, Amin MA and Koch AE: Macrophage migration inhibitory factor promotes tumor growth in the context of lung injury and repair. Am J Resp Crit Care. 182:1030–1037. 2010. View Article : Google Scholar

15 

Gámez-Pozo A, Sánchez-Navarro I, Calvo E, Agulló-Ortuño MT, López-Vacas R, Díaz E, Camafeita E, Nistal M, Madero R, Espinosa E, et al: PTRF/Cavin-1 and MIF proteins are identified as non-small cell lung cancer biomarkers by label-free proteomics. PLoS One. 7:e337522012. View Article : Google Scholar : PubMed/NCBI

16 

McClelland M, Zhao L, Carskadon S and Arenberg D: Expression of CD74, the receptor for macrophage migration inhibitory factor, in non-small cell lung cancer. Am J Pathol. 174:638–646. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Tanese K, Hashimoto Y, Berkova Z, Wang Y, Samaniego F, Lee JE, Ekmekcioglu S and Grimm EA: Cell Surface CD74-MIF interactions drive melanoma survival in response to interferon-γ. J Invest Dermatol. 135:2775–2784. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Gordon-Weeks AN, Lim SY, Yuzhalin AE, Jones K and Muschel R: Macrophage migration inhibitory factor: A key cytokine and therapeutic target in colon cancer. Cytokine Growth Factor Rev. 26:451–461. 2015. View Article : Google Scholar : PubMed/NCBI

19 

Chang KP, Lin SJ, Liu SC, Yi JS, Chien KY, Chi LM, Kao HK, Liang Y, Lin YT, Chang YS and Yu JS: Low-molecular-mass secretome profiling identifies HMGA2 and MIF as prognostic biomarkers for oral cavity squamous cell carcinoma. Sci Rep. 5:116892015. View Article : Google Scholar : PubMed/NCBI

20 

Verjans E, Noetzel E, Bektas N, Schütz AK, Lue H, Lennartz B, Hartmann A, Dahl E and Bernhagen J: Dual role of macrophage migration inhibitory factor (MIF) in human breast cancer. BMC Cancer. 9:2302009. View Article : Google Scholar : PubMed/NCBI

21 

Richard V, Kindt NG, Decaestecker C, Gabius HJ, Laurent G, Noël JC and Saussez S: Involvement of macrophage migration inhibitory factor and its receptor (CD74) in human breast cancer. Oncol Rep. 32:523–529. 2014.PubMed/NCBI

22 

Kindt N, Lechien JR, Nonclercq D, Laurent G and Saussez S: Involvement of CD74 in head and neck squamous cell carcinomas. J Cancer Res Clin. 140:937–947. 2014. View Article : Google Scholar

23 

Mittelbronn M, Platten M, Zeiner P, Dombrowski Y, Frank B, Zachskorn C, Harter PN, Weller M and Wischhusen J: Macrophage migration inhibitory factor (MIF) expression in human malignant gliomas contributes to immune escape and tumour progression. Acta Neuropathol. 122:353–365. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Krockenberger M, Dombrowski Y, Weidler C, Ossadnik M, Hönig A, Häusler S, Voigt H, Becker JC, Leng L, Steinle A, et al: Macrophage migration inhibitory factor (MIF) contributes to the immune escape of ovarian cancer by downregulating NKG2D1. J Immunol. 180:7338–7348. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Zhou Q, Yan X, Gershan J, Orentas RJ and Johnson BD: Expression of macrophage migration inhibitory factor by neuroblastoma leads to the inhibition of antitumor T cell reactivity in vivo. J Immunol. 181:1877–1886. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Pillai MM, Iwata M, Awaya N, Graf L and Torok-Storb B: Monocyte-derived CXCL7 peptides in the marrow microenvironment. Blood. 107:3520–3526. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Pecks U, Kirschner I, Wölter M, Schlembach D, Koy C, Rath W and Glocker MO: Mass spectrometric profiling of cord blood serum proteomes to distinguish infants with intrauterine growth restriction from those who are small for gestational age and from control individuals. Transl Res. 164:57–69. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Ehlken C, Grundel B, Michels D, Junker B, Stahl A, Schlunck G, Hansen LL, Feltgen N, Martin G, Agostini HT and Pielen A: Increased expression of angiogenic and inflammatory proteins in the vitreous of patients with ischemic central retinal vein occlusion. PLoS One. 10:e01268592015. View Article : Google Scholar : PubMed/NCBI

29 

Yeo L, Adlard N, Biehl M, Juarez M, Smallie T, Snow M, Buckley CD, Raza K, Filer A and Scheel-Toellner D: Expression of chemokines CXCL4 and CXCL7 by synovial macrophages defines an early stage of rheumatoid arthritis. Ann Rheum Dis. 75:763–771. 2016. View Article : Google Scholar : PubMed/NCBI

30 

Ghasemzadeh M, Kaplan ZS, Alwis I, Schoenwaelder SM, Ashworth KJ, Westein E, Hosseini E, Salem HH, Slattery R, McColl SR, et al: The CXCR1/2 ligand NAP-2 promotes directed intravascular leukocyte migration through platelet thrombi. Blood. 121:4555–4566. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Giuliano S, Guyot M, Grépin R and Pagès G: The ELR+CXCL chemokines and their receptors CXCR1/CXCR2: A signaling axis and new target for the treatment of renal cell carcinoma. OncoImmunology. 3:e283992014. View Article : Google Scholar : PubMed/NCBI

32 

Matsubara J, Honda K, Ono M, Tanaka Y, Kobayashi M, Jung G, Yanagisawa K, Sakuma T, Nakamori S, Sata N, et al: Reduced plasma level of CXC chemokine ligand 7 in patients with pancreatic cancer. Cancer Epidemiol Biomarkers Prev. 20:160–171. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Tang Z, Yu M, Miller F, Berk RS, Tromp G and Kosir MA: Increased invasion through basement membrane by CXCL7-transfected breast cells. Am J Surg. 196:690–696. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Grépin R, Guyot M, Giuliano S, Boncompagni M, Ambrosetti D, Chamorey E, Scoazec JY, Negrier S, Simonnet H and Pagès G: The CXCL7/CXCR1/2 axis is a key driver in the growth of clear cell renal cell carcinoma. Cancer Res. 74:873–883. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Desurmont T, Skrypek N, Duhamel A, Jonckheere N, Millet G, Leteurtre E, Gosset P, Duchene B, Ramdane N, Hebbar M, et al: Overexpression of chemokine receptor CXCR2 and ligand CXCL7 in liver metastases from colon cancer is correlated to shorter disease-free and overall survival. Cancer Sci. 106:262–269. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Xu L, Li Y, Sun H, Zhen X, Qiao C, Tian S and Hou T: Current developments of macrophage migration inhibitory factor (MIF) inhibitors. Drug Discov Today. 18:592–600. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Guo F, Zhang J, Wang L, Zhao W, Yu J, Zheng S and Wang J: Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncol Lett 14: 687-694, 2017.
APA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., & Wang, J. (2017). Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncology Letters, 14, 687-694. https://doi.org/10.3892/ol.2017.6261
MLA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14.1 (2017): 687-694.
Chicago
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14, no. 1 (2017): 687-694. https://doi.org/10.3892/ol.2017.6261
Copy and paste a formatted citation
x
Spandidos Publications style
Guo F, Zhang J, Wang L, Zhao W, Yu J, Zheng S and Wang J: Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncol Lett 14: 687-694, 2017.
APA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., & Wang, J. (2017). Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes. Oncology Letters, 14, 687-694. https://doi.org/10.3892/ol.2017.6261
MLA
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14.1 (2017): 687-694.
Chicago
Guo, F., Zhang, J., Wang, L., Zhao, W., Yu, J., Zheng, S., Wang, J."Identification of differentially expressed inflammatory factors in Wilms' tumors and their association with patient outcomes". Oncology Letters 14, no. 1 (2017): 687-694. https://doi.org/10.3892/ol.2017.6261
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team