How to detect the rare BCR‑ABL (e14a3) transcript: A case report and literature review

  • Authors:
    • Lin‑Hui Hu
    • Lian‑Fang Pu
    • Dong‑Dong Yang
    • Cui Zhang
    • Hui‑Ping Wang
    • Yang‑Yang Ding
    • Man‑Man Li
    • Zhi‑Min Zhai
    • Shudao Xiong
  • View Affiliations

  • Published online on: August 28, 2017     https://doi.org/10.3892/ol.2017.6847
  • Pages: 5619-5623
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Abstract

The Philadelphia (Ph; BCR‑ABL) chromosome originates from a translocation event between chromosomes 9 and 22, and results in the BCR‑ABL fusion gene. In chronic myelogenous leukemia (CML), the BCR‑ABL gene is mainly coded for by a major breakpoint cluster region (M‑bcr, e13a2 and e14a2). However, in some patients, BCR‑ABL genes are encoded by a minor (m)‑bcr, e1a2, and a micro (µ)‑bcr region, e19a2. These transcripts revealed a different clinical course. The present study described a CML patient whose cytogenetics and FISH analyses of bone marrow revealed a karyotype of 46, XY t(9,22) (q34;q11), while the commercial kits of quantitative PCR (qPCR) failed to detect the BCR‑ABL fusion gene. Further multiplex Reverse transcription‑PCR (RT‑PCR) and sequencing analyses identified a rare e14a3 (b3a3) fusion transcript.

Introduction

A translocation event that occurs between chromosomes 9 and 22, t(9;22)(q34;q11), results in the Philadelphia (Ph) chromosome, and consequently the BCR-ABL fusion gene, which is transcribed and translated into a hybrid protein (1). This fusion gene comprises almost the entire coding region of the Abelson (ABL) gene on chromosome 9, and the changeable coding region of the breakpoint cluster region (BCR) gene on chromosome 22 (2). In chronic myelogenous leukemia (CML), the breakpoint region lies between exons 12 and 16, and is termed the major breakpoint cluster region (M-bcr). The translocation events involving the M-bcr transcripts e13a2 (b2a2) and e14a2 (b3a2) encode a 210 kDa protein termed P210. Translocations that occur within the minor(m)-bcr region retain only the first exon of the BCR gene transcripts, e1a2, which yields a smaller P190 gene product. A rare P230 fusion protein, with a micro (µ)-bcr breakpoint between exons 19 and 20, has also been described in a number of variant cases (3). In addition, some rare breakpoints have also been sporadically reported, which may be grouped into 4 categories: The BCR breakpoints originating within introns that lie outside the M-bcr, m-bcr, or µ-bcr fused to ABL a2; the BCR breakpoints occurring within exons fused to ABL a2; the typical BCR breakpoints M-bcr, m-bcr, or µ-bcr fused to ABL breakpoints located downstream of a2; and the transcripts containing intervening sequences between BCR and ABL a2 (4).

The National Comprehensive Cancer Network (NCCN) practice guidelines recommend that cytogenetics, fluorescence in situ hybridization (FISH) and quantitative polymerase chain reaction (qPCR) are used for the diagnosis of the chronic phase of adult CML (5). Cytogenetics and FISH detect the translocation event, yet cannot determinate the breakpoints on the chromosome, whereas qPCR is able to detect these breakpoints. However, special primers or probes are required when qPCR is used to detect these breakpoints on the chromosome. Numerous qPCR probes and primers have been designed to detect the common BCR-ABL transcript, but false negative results may present in patients with atypical transcripts.

The present study reported the data from a CML patient whose cytogenetics and FISH analyses of bone marrow revealed a karyotype of 46, XY, t(9,22)(q34;q11); however, qPCR failed to detect the BCR-ABL fusion gene. The PCR products by multiplex RT-PCR assay identified a band of ~300 bp, which were subsequently confirmed as the BCR-ABL e14a3 transcript through sequence analysis. In addition, the biological role of the rare fusion event was reviewed, and the ability of qPCR assays to detect this rare transcript was discussed in the present study.

Case report

A 40 year-old male, whose white blood cell (WBC) count had been elevated for several days, was referred to The Second Hospital of Anhui Medical University, Hefei, China, in November 2015 for evaluation of his markedly high leukocyte level of 46.42×109/l. No fever, hidrosis and musculoskeletal pain were found in the course of his physical examination. The peripheral blood test revealed hemoglobin (HB) levels of 132 g/l, a platelet count (PLT) of 275×109/l (67.6% neutrophils, 21.3% lymphocytes and 9.2% basophil cells). The bone marrow examination revealed marked hyper-cellularity, with an increased number of megakaryocytes and evidence of myeloid hyperplasia and basophilia. The chromosomal analysis presented a karyotype of 46, XY, t(9;22)(q34;q11). The FISH analysis produced a positive result for the BCR-ABL translocation. Based on these findings the patient was diagnosed with CML, and was treated with 400 mg/day imatinib. The patient appeared to respond well to this treatment, as demonstrated in Fig. 1.

Materials and methods

Cytogenetic analysis was performed on bone marrow aspirates according to the standard laboratory protocol. A total of twenty G-banded metaphases and two karyotypes were evaluated. LSI BCR/ABL dual color, dual fusion translocation probe set (Abbott Molecular Inc., Des Plaines, IL, USA) was used to performed FISH analysis, following the protocol of the manufacturer. Total RNA was extracted from EDTA-anticoagulated bone marrow samples using an UNlQ-10 Column RNA Isolation kit (Sangon Biotech Co., Ltd., Shanghai, China), following the protocol of the manufacturer. A multiplex RT-PCR was carried out as described previously (6). A qPCR assay was performed to detect the BCR-ABL fusion gene, using a quantitative fluorescent RNA detection kit (Shanghai Shenyou Bio Technology Co., Ltd., Shanghai, China). The human leukemia sup-b15 (e1a2), nalm-6 (BCR-ABL negative) cell lines, were purchased from JENNIO Biological Technology (Guangzhou, China), and BV173 (e13a2) cell line, was provided by Dr Mengqing Gao (Shanghai Jiao Tong University School of Medicine, Shanghai, China), were used as controls. Multiplex RT-PCR products were sequenced by Sangon Biotech Co., Ltd. The sequence of Multiplex RT-PCR product was clarified by BLASTN (National Center for Biotechnology Information; blast.ncbi.nlm.nih.gov/Blast.cgi). All the data of patient were collected according to the approval of the institutional review board and informed consent was obtained.

Results

The chromosomal analysis of the patient presented a karyotype of 46, XY, t(9;22)(q34;q11) in all 20 metaphases examined, as demonstrated in Fig. 2. FISH analysis detected the BCR-ABL translocation, as displayed in Fig. 3. Commercial kits of qPCR failed to detect the BCR-ABL fusion gene. The multiplex RT-PCR performed on the RNA did not detect the classic BCR-ABL P210 transcript in the cells obtained from the bone marrow of the patient, or the sup-b15 or nalm-6 cell lines, but was detected in the BV173 cell line with the e13a2 transcript. However, the multiplex RT-PCR product of the patient demonstrated a band measuring ~300 bp in 2% agarose gel, as demonstrated in Fig. 4. The multiplex RT-PCR product was sequenced by Sangon Biotech Co., Ltd., and confirmed the presence of the e14a3 BCR-ABL transcript (b3a3) by BLASTN analysis, which is showed in Fig. 5.

Discussion

In patients with CML with the Ph chromosomal abnormality, almost all fusion transcripts are e13a2 and e14a2 (M-bcr), which produce a 210 kDa protein when transcribed (7). In rare variant patients, e1a2 (m-bcr) and e19a2 (µ-bcr) transcripts are found. The breakpoint of these transcripts usually occurs in ABL exon2, but occasionally arises in ABL exon3. The protein transcribed by exon2 contains tyrosine kinase (SH1), SH2 and SH3 domains. In comparison with the proteins transcripted by exon2, the structure of the protein transcribed by exon3 lacked two-thirds of the N-terminal in the SH3 domain. A previous study determined that in vivo, SH3 domains negatively regulate the kinase domain (SH1) and activate the Signal transducer and activator of transcription 5-signaling pathway, which is an essential leukemogenic factor (8). Furthermore, mouse models have demonstrated lower tissue invasiveness and longer leukemic cell cycles when BCR-ABL mutants lack the SH3 domain (9). Consequently, it is expected that patients who exhibit BCR-ABL without exon2 may present with a different clinical course.

To our knowledge, there have been 14 reports, including the patient of the present study, of patients with the BCR-ABL e14a3 transcripts (5,1015) (Table I). The median age of these patients is 44.5 years old: Patients with BCR-ABL e14a3 transcripts appear to be younger than patients with the common transcript at the time of diagnosis. Notably, 12 patients were male, suggesting that the BCR-ABL e14a3 transcript is more common in males. In addition, these 14 patients exhibited a less aggressive form of the disease and lower WBC counts, ranging from 9–300×109/l. It was also noted that the deletion of exon2 did not affect the curative effects of imatinib, and 7 patients appeared to respond well to treatment with this drug (5,10). The patient in the present study also appeared to have a good response to imatinib. Combining these data with previous studies (5,10,16), patients with the BCR-ABL e14a3 transcript may expect a good clinical course.

Table I.

Summary of CML patients and the respective BCR-ABL e14a3 transcripts.

Table I.

Summary of CML patients and the respective BCR-ABL e14a3 transcripts.

Case no.Age/genderPhase of CMLWBC count (×109/l)Chromosome karyotypeBCR-ABL transcriptClinical outcomeDuration of follow-up (months)SplenomegalyReferences
  139/FCP9T (9,22)13QE14A3AliveUnknownNA(10)
  219/MCP42T (9,22)E14A3Dead34NA(11)
  323/MCP95.8T (9,22)E14A3Alive60NO(12)
  451/MCPNAT (9,22)E14A3Alive36NO(16)
  569/MCP18T (4,9,22)E14A3Alive126NO(16)
  669/MCP29T (9,22)E14A3AliveUnknownNO(13)
  781/MCP28T (9,22)E14A3AliveUnknownNA(5)
  830/MCPNAT (9,22)E14A3AliveUnknownNO(14)
  952/MCP229T (9,22)E14A3Alive112YES(9)
1041/MAP26T (9,22)E14A3Alive66YES(9)
1141/MCP115T (9,22)E14A3Alive101YES(9)
1248/FCP300T (9,22)E14A3Dead53YES(9)
1348/MCP98.2T (9,22)E14A3Alive30YES(9)
1440/MCP46T (9,22)E14A3Alive2NOPresent study

[i] M, male; F, female; CP, chronic phase, CML; AP, accelerated phase; CML, chronic myeloid leukemia; WBC, white blood cell; BCR-ABL, Philadelphia chromosome.

The present study observed that the multiplex RT-PCR produced negative results for e13a2 and e14a2, but exhibited a band measuring ~300 bp in the agarose gel. qPCR analysis to detect the BCR-ABL fusion gene was then performed, but this failed to detect the BCR-ABL fusion gene. However, the Ph chromosome was detected by cytogenetics and FISH analyses. To additionally investigate the discordant result, the multiplex RT-PCR products were sequenced and analyzed by BLASTN, which confirmed the atypical transcript e14a3 in the patient of the present study. Although qPCR failed to detect this transcript, the multiplex RT-PCR assay previously described succeeded in detecting the BCR-ABL e14a3 transcript. Based on these findings, the present study suggested that multiplex RT-PCR assay reported by Pallisgaard et al (6) may provide evidence that PCR products amplified by R6B group presents a band with ~300 bp, and associated with BCR-ABL e14a3 transcript.

The NCCN practice guidelines recommend that cytogenetics, FISH and qPCR be performed to diagnose the chronic phase of adult CML. In the present study, cytogenetics and FISH were successful in detecting the BCR-ABL e14a3 transcript in the patient. However, the qPCR failed, due to a lack of specific primers, which makes repeating the qPCR every 3–6 months, as recommend by the NCCN, useless. Presently, some commercial qPCR kits are able to detect the e14a3 and e13a3 transcripts using the reverse primer located in exon 4 of ABL, such as Roche Holding AG, Basel, Switzerland and Applied Biosystems, Thermo Fisher Scientific Co., Waltham, MA, USA (5), while special primers that are able to detect the e14a3 transcript have also been designed (17). However, these assays fail to distinguish the type of BCR-ABL transcript present. Different BCR-ABL transcripts result in different clinical courses (18), and the impact of the type of transcript on response and survival rate subsequent to initial treatment with different tyrosine kinase inhibitors demonstrates different outcomes (19). True categorization of the BCR-ABL transcript by breakpoint is required in order to investigate and comprehend the different types of CML, and thus provides accurate guides for the treatment and prognosis of the disease. In conclusion, a qPCR assay using commercial kits or primers designed by previous studies, which would detect the exon a3 transcripts, in order to estimate the disease progression of CML in a patient with the e14a3 transcript. Furthermore, in the future, multiplex RT-PCR assays may be applied to distinguish the type of BCR-ABL transcript present, with different transcripts demonstrating different sizes. Also, it is necessary to explore the possibility of a new qPCR assay that detects and distinguishes different BCR-ABL transcripts e13a2, e14a2, e13a3 and e14a3 respectively.

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Spandidos Publications style
Hu LH, Pu LF, Yang DD, Zhang C, Wang HP, Ding YY, Li MM, Zhai ZM and Xiong S: How to detect the rare BCR‑ABL (e14a3) transcript: A case report and literature review. Oncol Lett 14: 5619-5623, 2017
APA
Hu, L., Pu, L., Yang, D., Zhang, C., Wang, H., Ding, Y. ... Xiong, S. (2017). How to detect the rare BCR‑ABL (e14a3) transcript: A case report and literature review. Oncology Letters, 14, 5619-5623. https://doi.org/10.3892/ol.2017.6847
MLA
Hu, L., Pu, L., Yang, D., Zhang, C., Wang, H., Ding, Y., Li, M., Zhai, Z., Xiong, S."How to detect the rare BCR‑ABL (e14a3) transcript: A case report and literature review". Oncology Letters 14.5 (2017): 5619-5623.
Chicago
Hu, L., Pu, L., Yang, D., Zhang, C., Wang, H., Ding, Y., Li, M., Zhai, Z., Xiong, S."How to detect the rare BCR‑ABL (e14a3) transcript: A case report and literature review". Oncology Letters 14, no. 5 (2017): 5619-5623. https://doi.org/10.3892/ol.2017.6847