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Article Open Access

Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells

  • Authors:
    • Huan Lu
    • Huili Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Huadong Hospital, Fudan University, Shanghai 200040, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5906-5912
    |
    Published online on: September 8, 2017
       https://doi.org/10.3892/ol.2017.6916
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Abstract

The aim of the present study was to investigate the effects of transketolase (TKT) on cell proliferation, cell migration and interaction with other metabolism‑associated genes in A549 lung cancer cells. A549 cells were transfected with three TKT‑specific small interfering (si)RNAs, screened for the optimal transfection concentration, and sequenced with flow cytometry and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Cell viability was evaluated using Cell Counting Kit‑8 (CCK‑8), cell cycle was assessed by flow cytometric analysis. Cell migration was determined by scratch‑wound and Transwell chamber assays. The changes in mRNA expression levels of glucose‑6‑phosphate dehydrogenase (G6PDH), transaldolase (TAL), sorbitol dehydrogenase (SORD), phosphoribosyl pyrophosphate synthetase 1 (PRPS1) and hexokinase 1 (HK1) were detected by RT‑qPCR. siRNA‑C at 50 nmol/l was selected for the subsequent experiments. Compared with the negative control, cell proliferation of the TKT‑siRNA‑C group was inhibited dramatically (CCK‑8 24 h, 0.2984±0.0371 vs. 0.0952±0.0063; P<0.0001), the cell cycle was arrested at the G1/G0 cell cycle phase (58±2.0% vs. 70±2.5%; P=0.002), and cell migration ability was decreased [wound size, 254.71±34.96 vs. 349.12±37.43 µm (P=0.0001); Transwell migration, 250±47.8/field vs. 150±49.0/field (P<0.0001)]. The mRNA expression levels of G6PDH, TAL, SORD, PRPS1 and HK1 were downregulated in the TKT‑siRNA‑C group compared with the negative control. The present study revealed that synthetic TKT‑siRNA can inhibit A549 cell viability and migration, which may be due to arrest of the cell cycle and downregulation of relevant metabolic enzymes.
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Copy and paste a formatted citation
Spandidos Publications style
Lu H and Zhu H: Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells. Oncol Lett 14: 5906-5912, 2017.
APA
Lu, H., & Zhu, H. (2017). Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells. Oncology Letters, 14, 5906-5912. https://doi.org/10.3892/ol.2017.6916
MLA
Lu, H., Zhu, H."Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells". Oncology Letters 14.5 (2017): 5906-5912.
Chicago
Lu, H., Zhu, H."Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells". Oncology Letters 14, no. 5 (2017): 5906-5912. https://doi.org/10.3892/ol.2017.6916
Copy and paste a formatted citation
x
Spandidos Publications style
Lu H and Zhu H: Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells. Oncol Lett 14: 5906-5912, 2017.
APA
Lu, H., & Zhu, H. (2017). Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells. Oncology Letters, 14, 5906-5912. https://doi.org/10.3892/ol.2017.6916
MLA
Lu, H., Zhu, H."Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells". Oncology Letters 14.5 (2017): 5906-5912.
Chicago
Lu, H., Zhu, H."Effect of siRNA‑mediated gene silencing of transketolase on A549 lung cancer cells". Oncology Letters 14, no. 5 (2017): 5906-5912. https://doi.org/10.3892/ol.2017.6916
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