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Article

MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a

  • Authors:
    • Jingbo He
    • Huixiong Qi
    • Fang Chen
    • Chuanhua Cao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Xiangyang Central Hospital, Hubei University of Arts and Science, Xiangyang, Hubei 441021, P.R. China, Department of Gerontology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
  • Pages: 6097-6102
    |
    Published online on: September 18, 2017
       https://doi.org/10.3892/ol.2017.6982
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Abstract

Gastric cancer (GC) is a common type of malignancy worldwide, and chemotherapeutic resistance accounts for the majority of the failures in clinical treatment. MicroRNAs (miRs) are a class of small non‑coding RNAs, which serve essential roles in GC. The present study aimed to investigate the potential role of miR‑25 in the cisplatin sensitivity of GC cells. The expression level of miR‑25 was significantly upregulated in the cisplatin‑resistant GC cell line SGC‑7901/DDP compared with the SGC‑7901 parental cell line. Overexpression of miR‑25 significantly enhanced cell cycle progression and decreased the sensitivity of SGC‑7901 cells to cisplatin, whereas inhibition of miR‑25 in the SGC‑7901/DDP cisplatin‑resistant cells resulted in cell cycle arrest at the G0/G1 phase and significantly increased drug sensitivity. Furthermore, the tumor suppressor forkhead box O3a (FOXO3a) was identified as a direct target gene of miR‑25 by luciferase assay and western blot analysis, and was shown to mediate the drug‑resistance phenotype of GC cells. These findings suggest that upregulation of miR‑25 is important for GC cells to establish a cisplatin‑resistant phenotype via a FOXO3a‑dependent mechanism. Therefore, targeting miR‑25 may be a promising therapeutic approach to treat patients with cisplatin‑resistant GC.
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Copy and paste a formatted citation
Spandidos Publications style
He J, Qi H, Chen F and Cao C: MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a. Oncol Lett 14: 6097-6102, 2017.
APA
He, J., Qi, H., Chen, F., & Cao, C. (2017). MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a. Oncology Letters, 14, 6097-6102. https://doi.org/10.3892/ol.2017.6982
MLA
He, J., Qi, H., Chen, F., Cao, C."MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a". Oncology Letters 14.5 (2017): 6097-6102.
Chicago
He, J., Qi, H., Chen, F., Cao, C."MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a". Oncology Letters 14, no. 5 (2017): 6097-6102. https://doi.org/10.3892/ol.2017.6982
Copy and paste a formatted citation
x
Spandidos Publications style
He J, Qi H, Chen F and Cao C: MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a. Oncol Lett 14: 6097-6102, 2017.
APA
He, J., Qi, H., Chen, F., & Cao, C. (2017). MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a. Oncology Letters, 14, 6097-6102. https://doi.org/10.3892/ol.2017.6982
MLA
He, J., Qi, H., Chen, F., Cao, C."MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a". Oncology Letters 14.5 (2017): 6097-6102.
Chicago
He, J., Qi, H., Chen, F., Cao, C."MicroRNA‑25 contributes to cisplatin resistance in gastric cancer cells by inhibiting forkhead box O3a". Oncology Letters 14, no. 5 (2017): 6097-6102. https://doi.org/10.3892/ol.2017.6982
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