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Article

Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression

  • Authors:
    • Fumin Zhang
    • Ming Wan
    • Yi Xu
    • Zhenglong Li
    • Pengcheng Kang
    • Xingming Jiang
    • Yimin Wang
    • Zhidong Wang
    • Xiangyu Zhong
    • Chunlong Li
    • Yunfu Cui
  • View Affiliations / Copyright

    Affiliations: Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150000, P.R. China, Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150000, P.R. China
  • Pages: 6079-6084
    |
    Published online on: September 18, 2017
       https://doi.org/10.3892/ol.2017.6987
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Abstract

The incidence of extrahepatic cholangiocarcinoma (ECC) is the highest of all the cholangiocarcinoma cases. However, the molecular mechanism of ECC genesis and progression remains unclear. Long non‑coding RNAs (lncRNAs) have been revealed to perform critical regulatory roles in cancer biology. In order to understand lncRNA expression patterns and their potential function in ECC, a transcriptome analysis of lncRNA and mRNA expression was performed in ECC and paired adjacent non‑cancerous tissues using Agilent human lncRNA + mRNA arrayV4.0 (4x180 K format). It was identified that 268 lncRNAs and 459 mRNAs were differentially expressed in ECC. Among these, 78 lncRNAs and 66 mRNAs were upregulated >2‑fold compared with adjacent non‑cancerous tissues, and 190 lncRNAs and 393 mRNAs were downregulated in the ECC samples. Differences in lncRNA expression between ECC and paired adjacent non‑cancerous tissues were confirmed using reverse transcription‑quantitative polymerase chain reactionas proof of principle. Functional analysis of co‑expressed mRNAs with lncRNAs indicated that these dysregulated lncRNAsmay be involved in known ECC‑associated biological processes and pathways. The present findings indicated that mRNAs and lncRNAs perform important roles in the development and progression of ECC. The present findings may lay the foundation for future efforts to understand the role of lncRNAs and develop novel biomarkers in ECC
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Copy and paste a formatted citation
Spandidos Publications style
Zhang F, Wan M, Xu Y, Li Z, Kang P, Jiang X, Wang Y, Wang Z, Zhong X, Li C, Li C, et al: Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression. Oncol Lett 14: 6079-6084, 2017.
APA
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X. ... Cui, Y. (2017). Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression. Oncology Letters, 14, 6079-6084. https://doi.org/10.3892/ol.2017.6987
MLA
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X., Wang, Y., Wang, Z., Zhong, X., Li, C., Cui, Y."Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression". Oncology Letters 14.5 (2017): 6079-6084.
Chicago
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X., Wang, Y., Wang, Z., Zhong, X., Li, C., Cui, Y."Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression". Oncology Letters 14, no. 5 (2017): 6079-6084. https://doi.org/10.3892/ol.2017.6987
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang F, Wan M, Xu Y, Li Z, Kang P, Jiang X, Wang Y, Wang Z, Zhong X, Li C, Li C, et al: Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression. Oncol Lett 14: 6079-6084, 2017.
APA
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X. ... Cui, Y. (2017). Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression. Oncology Letters, 14, 6079-6084. https://doi.org/10.3892/ol.2017.6987
MLA
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X., Wang, Y., Wang, Z., Zhong, X., Li, C., Cui, Y."Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression". Oncology Letters 14.5 (2017): 6079-6084.
Chicago
Zhang, F., Wan, M., Xu, Y., Li, Z., Kang, P., Jiang, X., Wang, Y., Wang, Z., Zhong, X., Li, C., Cui, Y."Transcriptome analysis reveals dysregulated long non‑coding RNAs and mRNAs associated with extrahepatic cholangiocarcinoma progression". Oncology Letters 14, no. 5 (2017): 6079-6084. https://doi.org/10.3892/ol.2017.6987
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