Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia

Gomes,Aurélie; Defaux,Matthieu; Lemee,Riwanon  Michel; Lobjois,Valérie; Ducommun,Bernard

doi:10.3892/ol.2017.7465

February-2018 Volume 15 Issue 2

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February-2018 Volume 15 Issue 2

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Article

Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia

Authors:
- Aurélie Gomes
- Matthieu Defaux
- Riwanon Michel Lemee
- Valérie Lobjois
- Bernard Ducommun
View Affiliations / Copyright

Affiliations: Institut des Technologies Avancées en Sciences du Vivant, Université de Toulouse and Centre National de la Recherche Scientifique, 31106 Toulouse, France, Atmosphère Contrôle SAS, 14760 Bretteville sur Odon, France, Laboratoires Standa, 14050 Caen, France
Pages: 2006-2009
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Published online on: November 21, 2017

https://doi.org/10.3892/ol.2017.7465
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Abstract

Multicellular tumor spheroids models are of increasing interest in preclinical studies and pharmacological evaluation. However, their storage and transport is often a limitation because it requires adapted and expensive procedures. Here, we propose a very simple method to store 3D spheroids, using a procedure based on oxygen absorber-induced anoxia. We report that oxygen absorbers allow generating an anoxic environment for spheroid storage in culture plates. Oxygen absorber-induced anoxia fully and reversibly arrests spheroid growth for 4 days at 37°C and up to 18 days at 4°C. We then show that the response to etoposide is comparable in spheroids preserved in conditions of absorber-induced anoxia at 4°C and spheroids kept in normoxia at 37°C. These results represent a major improvement that should simplify the storage, transport and use of 3D spheroids.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Reversible growth arrest of 3D tumor spheroids stored in oxygen absorber-induced anoxia

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Abstract

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