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Article Open Access

Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment

  • Authors:
    • Song Qin
    • Zhipeng Gao
    • Yu Liu
    • Changbai Liu
    • Jun Wang
    • Li Li Zou
  • View Affiliations / Copyright

    Affiliations: Translational Neuroscience and Neural Regeneration and Repair Institute/Institute of Cell Therapy, The First Hospital of Yichang, China Three Gorges University, Yichang, Hubei 443002, P.R. China, Department of Microbiology and Immunology, Medical College, China Three Gorges University, Yichang, Hubei 443000, P.R. China
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1630-1638
    |
    Published online on: November 23, 2017
       https://doi.org/10.3892/ol.2017.7477
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Abstract

In cancer immunotherapy, dendritic cell (DC)‑based vaccines represent a promising, yet challenging, treatment method. In addition to overcoming the low expression levels of antigenic epitopes on cancer cells, it is also necessary to overcome the inhibitory effect of suppressor of cytokine signaling 1 (SOCS1) on DC self‑antigen presentation. Our group previously demonstrated that calreticulin (CRT) translocated type I transmembrane glycoprotein mucin 1 (MUC1), a breast cancer antigen, to the surface of 4T1 cells, and that treatment with MUC1‑CRT‑primed 4T1 cell‑treated DCs induced apoptosis in a breast cancer cell line. In the present study, cell penetrate peptide, hpp10‑DRBD was successfully used to deliver siRNAs into bone marrow‑derived (BM) DCs to construct SOCS1‑silenced DCs, which were incubated with MUC1‑CRT‑primed 4T1 cells, and antigen‑specific antitumor immunity was markedly enhanced in vitro and in vivo. These results demonstrated that SOCS1‑silencing, combined with MUC1‑CRT‑primed 4T1 cell treatment, may induce increased cytokine production and T cell proliferation by DCs. Furthermore, the in vivo experimental data demonstrated that the silencing of SOCS1 combined with MUC1‑CRT‑primed 4T1 treatment of BMDCs may induce enhanced immunological effects. The results of the present study have implications for the development of more effective DC‑based tumor vaccines, suggesting that the combination of high tumor‑associated antigen expression levels on cancer cells with the silencing of a critical inhibitor of DC antigen presentation may be beneficial.
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Copy and paste a formatted citation
Spandidos Publications style
Qin S, Gao Z, Liu Y, Liu C, Wang J and Zou LL: Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment. Oncol Lett 15: 1630-1638, 2018.
APA
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., & Zou, L.L. (2018). Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment. Oncology Letters, 15, 1630-1638. https://doi.org/10.3892/ol.2017.7477
MLA
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., Zou, L. L."Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment". Oncology Letters 15.2 (2018): 1630-1638.
Chicago
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., Zou, L. L."Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment". Oncology Letters 15, no. 2 (2018): 1630-1638. https://doi.org/10.3892/ol.2017.7477
Copy and paste a formatted citation
x
Spandidos Publications style
Qin S, Gao Z, Liu Y, Liu C, Wang J and Zou LL: Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment. Oncol Lett 15: 1630-1638, 2018.
APA
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., & Zou, L.L. (2018). Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment. Oncology Letters, 15, 1630-1638. https://doi.org/10.3892/ol.2017.7477
MLA
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., Zou, L. L."Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment". Oncology Letters 15.2 (2018): 1630-1638.
Chicago
Qin, S., Gao, Z., Liu, Y., Liu, C., Wang, J., Zou, L. L."Silencing of suppressor of cytokine signaling 1 enhances the immunological effect of mucin 1-calreticulin-primed 4T1 cell-treated dendritic cells in breast cancer treatment". Oncology Letters 15, no. 2 (2018): 1630-1638. https://doi.org/10.3892/ol.2017.7477
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