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Article

Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells

  • Authors:
    • Zhenyu Hou
    • Yunfeng Cui
    • Huizhi Xing
    • Xiaoyan Mu
  • View Affiliations / Copyright

    Affiliations: The Second Department of Surgery, Nankai Hospital, Tianjin Medical University, Tianjin 300162, P.R. China, Department of General Surgery, The Affiliated Hospital, Logistics University of CATF, Tianjin 300162, P.R. China
  • Pages: 1943-1948
    |
    Published online on: November 29, 2017
       https://doi.org/10.3892/ol.2017.7500
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Abstract

The present study investigated whether poly(ADP‑ribose) polymerase (PARP) has an effect on p53‑regulated pancreatic cancer. The results of the present study demonstrated that the expression of PARP affects proliferation and apoptosis of pancreatic cancer cells. Olaparib was used to suppress the expression level of PARP‑1 in PanC‑1 cells. Decreased expression of PARP‑1 suppressed cell proliferation and induced apoptosis of PanC‑1 cells when compared with controls. Furthermore, decreased expression of PARP‑1 resulted in decreased levels of pro‑caspase‑3 expression, increased caspase‑3 activity, suppressed B‑cell lymphoma 2 (Bcl‑2) protein expression and increased p53 protein expression in PanC‑1 cells. Subsequently, ataxia telangiectasia mutated (ATM) activity was inhibited alongside down‑expression of PARP‑1 resulting in significantly decreased cellular viability of PanC‑1 cells, increased p53 protein expression, decreased expression of pro‑caspase‑3, increased caspase‑3 activity and suppressed Bcl‑2 protein expression, when compared with PARP‑1 suppression alone. Overall, the in vitro data confirmed that down‑expression of PARP‑1 suppressed cell proliferation and induced apoptosis of pancreatic cancer via ATM‑deficient p53 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Hou Z, Cui Y, Xing H and Mu X: Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells. Oncol Lett 15: 1943-1948, 2018.
APA
Hou, Z., Cui, Y., Xing, H., & Mu, X. (2018). Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells. Oncology Letters, 15, 1943-1948. https://doi.org/10.3892/ol.2017.7500
MLA
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15.2 (2018): 1943-1948.
Chicago
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15, no. 2 (2018): 1943-1948. https://doi.org/10.3892/ol.2017.7500
Copy and paste a formatted citation
x
Spandidos Publications style
Hou Z, Cui Y, Xing H and Mu X: Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells. Oncol Lett 15: 1943-1948, 2018.
APA
Hou, Z., Cui, Y., Xing, H., & Mu, X. (2018). Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells. Oncology Letters, 15, 1943-1948. https://doi.org/10.3892/ol.2017.7500
MLA
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15.2 (2018): 1943-1948.
Chicago
Hou, Z., Cui, Y., Xing, H., Mu, X."Down‑expression of poly(ADP‑ribose) polymerase in p53‑regulated pancreatic cancer cells". Oncology Letters 15, no. 2 (2018): 1943-1948. https://doi.org/10.3892/ol.2017.7500
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