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Article

A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters

  • Authors:
    • Feng Liu
    • Kaya Xu
    • Hua Yang
    • Yuming Li
    • Jian Liu
    • Jixiang Wang
    • Zhizhong Guan
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Hospital Affiliated to Guizhou Medical University, Guiyang, Guizhou 550000, P.R. China, Department of Molecular Biology, The Laboratory of Molecular Biology, Guizhou Medical University, Guiyang, Guizhou 550000, P.R. China
  • Pages: 3362-3368
    |
    Published online on: December 21, 2017
       https://doi.org/10.3892/ol.2017.7684
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Abstract

Gliomas are the most common type of primary brain tumor in adults, where more than half of the cases are malignant, and the prognosis is poor. The early viral 1A (E1A) protein has been widely recognized to be essential for adenoviral replication and production of progeny virions in human cells, a process that is regulated by human telomerase reverse transcriptase. The p53 gene, as a tumor suppressor, regulates diverse cellular processes, including cell cycle arrest, cell autophagy, senescence and apoptosis. Dysfunction of the p53 pathways is common in malignant gliomas. Exogenous expression of p53 during adenovirus replication in human cancer cells may accelerate cell death and improve the release of early virus progeny. In the present study, a conditionally replicative adenovirus (CRAd) Ad‑Tp‑E1A‑Gp‑p53, which expressed functional p53 protein when replicating in cancer cells, was constructed. Next, the level of p53 expression in U251 cells was determined by western blot analysis, and the inhibitory effect of Ad‑Tp‑E1A‑Gp‑p53 on U251 cells was detected via an MTT assay. The results indicated that p53 expression was upregulated with an increase in the multiplicity of infection (MOI) of Ad‑Tp‑E1A‑Gp‑p53. Additionally, the inhibitory effects of Ad‑Tp‑E1A‑Gp‑p53 in different groups were significantly different (P<0.05), with the inhibition ratio of the experimental groups being higher, compared with the control group (P<0.05). Furthermore, the inhibition ratio increased with increases in the MOI of Ad‑Tp‑E1A‑Gp‑p53. Therefore, the expression of functional p53 and that of E1A may increase the potency of CRAd, and overexpression of p53 through CRAd is a promising approach to more effective treatments in a number of human cancer types.
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1 

Ulasov I, Borovjagin AV, Kaverina N, Schroeder B, Shah N, Lin B, Baryshnikov A and Cobbs C: MT1-MMP silencing by an shRNA-armed glioma-targeted conditionally replicative adenovirus (CRAd) improves its anti-glioma efficacy in vitro and in vivo. Cancer Lett. 365:240–250. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Gilbert MR, Dignam JJ, Armstrong TS, Wefel JS, Blumenthal DT, Vogelbaum MA, Colman H, Chakravarti A, Pugh S, Won M, et al: A randomized trial of bevacizumab for newly diagnosed glioblastoma. N Engl J Med. 370:699–708. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Westphal M, Ylä-Herttuala S, Martin J, Warnke P, Menei P, Eckland D, Kinley J, Kay R and Ram Z; ASPECT Study Group, : Adenovirus-mediated gene therapy with sitimagene ceradenovec followed by intravenous ganciclovir for patients with operable high-grade glioma (ASPECT): a randomised, open-label, phase 3 trial. Lancet Oncol. 14:823–833. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Holland EC: Glioblastoma multiforme: The terminator. Proc Natl Acad Sci USA. 97:pp. 6242–6244. 2000; View Article : Google Scholar : PubMed/NCBI

5 

Nemunaitis J and Edelman J: Selectively replicating viral vectors. Cancer Gene Ther. 9:987–1000. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Geoerger B, Vassal G, Opolon P, Dirven CM, Morizet J, Laudani L, Grill J, Giaccone G, Vandertop WP, Gerritsen WR and van Beusechem VW: Oncolytic activity of p53-expressing conditionally replicative adenovirus AdDelta24-p53 against human malignant glioma. Cancer Res. 64:5753–5759. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Wei F, Wang H, Chen X, Li C and Huang Q: Dissecting the roles of E1A and E1B in adenoviral replication and RCAd-enhanced RDAd transduction efficacy on tumor cells. Cancer Biol Ther. 15:1358–1366. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Alemany R, Balagué C and Curiel DT: Replicative adenoviruses for cancer therapy. Nat Biotechnol. 18:723–727. 2000. View Article : Google Scholar : PubMed/NCBI

9 

van Beusechem VW, van den Doel PB, Grill J, Pinedo HM and Gerritsen WR: Conditionally replicative adenovirus expressing p53 exhibits enhanced oncolytic potency. Cancer Res. 62:6165–6171. 2002.PubMed/NCBI

10 

Fukuda K, Abei M, Ugai H, Kawashima R, Seo E, Wakayama M, Murata T, Endo S, Hamada H, Hyodo I and Yokoyama KK: E1A, E1B double-restricted replicative adenovirus at low dose greatly augments tumor-specific suicide gene therapy for gallbladder cancer. Cancer Gene Ther. 16:126–136. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Xu J, Mo Y, Wang X, Liu J, Zhang X, Wang J, Hu L, Yang C, Chen L and Wang Y: Conditionally replicative adenovirus-based mda-7/IL-24 expression enhances sensitivity of colon cancer cells to 5-fluorouracil and doxorubicin. J Gastroenterol. 48:203–213. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Chu RL, Post DE, Khuri FR and Van Meir EG: Use of replicating oncolytic adenoviruses in combination therapy for cancer. Clin Cancer Res. 10:5299–5312. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Chiocca EA: Oncolytic viruses. Nature Rev Cancer. 2:938–950. 2002. View Article : Google Scholar

14 

Fueyo J, Alemany R, Gomez-Manzano C, Fuller GN, Khan A, Conrad CA, Liu TJ, Jiang H, Lemoine MG, Suzuki K, et al: Preclinical characterization of the antiglioma activity of a tropism-enhanced adenovirus targeted to the retinoblastoma pathway. J Natl Cancer Inst. 95:652–660. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Geoerger B, Grill J, Opolon P, Morizet J, Aubert G, Terrier-Lacombe MJ, Bressac De-Paillerets B, Barrois M, Feunteun J, Kirn DH and Vassal G: Oncolytic activity of the E1B-55 kDa-deleted adenovirus ONYX-015 is independent of cellular p53 status in human malignant glioma xenografts. Cancer Res. 62:764–772. 2002.PubMed/NCBI

16 

Lamfers ML, Grill J, Dirven CM, Van Beusechem VW, Geoerger B, van den Berg J, Alemany R, Fueyo J, Curiel DT, Vassal G, et al: Potential of the conditionally replicative adenovirus Ad5-Delta24RGD in the treatment of malignant gliomas and its enhanced effect with radiotherapy. Cancer Res. 62:5736–5742. 2002.PubMed/NCBI

17 

Wirth T, Kuhnel F and Kubicka S: Telomerase-dependent gene therapy. Curr Mol Med. 5:243–251. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Wirth T, Zender L, Schulte B, Mundt B, Plentz R, Rudolph KL, Manns M, Kubicka S and Kühnel F: A telomerase-dependent conditionally replicating adenovirus for selective treatment of cancer. Cancer Res. 63:3181–3188. 2003.PubMed/NCBI

19 

Alemany R: Conditionally replicating adenoviruses for cancer treatment. Cancer Gene Ther. 1–248. 2005.PubMed/NCBI

20 

Wilhelmsson U, Eliasson C, Bjerkvig R and Pekny M: Loss of GFAP expression in high-grade astrocytomas does not contribute to tumor development or progression. Oncogene. 22:3407–3411. 2003. View Article : Google Scholar : PubMed/NCBI

21 

Lee K, Jeon K, Kim JM, et al: Downregulation of GFAP, TSP-1 and p53 in human glioblastoma cell line, U373MG, by IE1 protein from human cytomegalovirus. Glia. 51:1–12. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Tazawa H, Kagawa S and Fujiwara T: Advances in adenovirus-mediated p53 cancer gene therapy. Expert Opin Biol Ther. 13:1569–1583. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Heise C, Hermiston T, Johnson L, Brooks G, Sampson-Johannes A, Williams A, Hawkins L and Kirn D: An adenovirus E1A mutant that demonstrates potent and selective systemic anti-tumoral efficacy. Nat Med. 6:1134–1139. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Collins V: Gene amplification in human gliomas. Glia. 15:289–296. 1995. View Article : Google Scholar : PubMed/NCBI

25 

Lin T, Wang M, Liang HS and Liu EZ: The expression of p53, mgmt and egfr in brain glioma and clinical significance. J Biol Regul Homeost Agents. 29:143–149. 2015.PubMed/NCBI

26 

Chen L, Zhang R, Li P, Liu Y, Qin K, Fa ZQ, Liu YJ, Ke YQ and Jiang XD: p53-induced microRNA-107 inhibits proliferation of glioma cells and down-regulates the expression of CDK6 and Notch-2. Neurosci Lett. 534:327–332. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Yang ZX, Wang D, Wang G, Zhang QH, Liu JM, Peng P and Liu XH: Clinical study of recombinant adenovirus-p53 combined with fractionated stereotactic radiotherapy for hepatocellular carcinoma. J Cancer Res Clin Oncol. 136:625–630. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu F, Xu K, Yang H, Li Y, Liu J, Wang J and Guan Z: A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters. Oncol Lett 15: 3362-3368, 2018.
APA
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., & Guan, Z. (2018). A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters. Oncology Letters, 15, 3362-3368. https://doi.org/10.3892/ol.2017.7684
MLA
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., Guan, Z."A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters". Oncology Letters 15.3 (2018): 3362-3368.
Chicago
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., Guan, Z."A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters". Oncology Letters 15, no. 3 (2018): 3362-3368. https://doi.org/10.3892/ol.2017.7684
Copy and paste a formatted citation
x
Spandidos Publications style
Liu F, Xu K, Yang H, Li Y, Liu J, Wang J and Guan Z: A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters. Oncol Lett 15: 3362-3368, 2018.
APA
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., & Guan, Z. (2018). A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters. Oncology Letters, 15, 3362-3368. https://doi.org/10.3892/ol.2017.7684
MLA
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., Guan, Z."A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters". Oncology Letters 15.3 (2018): 3362-3368.
Chicago
Liu, F., Xu, K., Yang, H., Li, Y., Liu, J., Wang, J., Guan, Z."A novel approach to glioma therapy using an oncolytic adenovirus with two specific promoters". Oncology Letters 15, no. 3 (2018): 3362-3368. https://doi.org/10.3892/ol.2017.7684
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