The effects of vitamin B6 compounds on cell proliferation and melanogenesis in B16F10 melanoma cells

  • Authors:
    • Taisuke Matsuo
    • Aki Fujiwara
    • Kazuhiro Nakamura
    • Yasuyuki Sadzuka
  • View Affiliations

  • Published online on: February 5, 2018     https://doi.org/10.3892/ol.2018.7947
  • Pages: 5181-5184
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Abstract

B16F10 murine melanoma cells are frequently used for the study of cancer and melanogenesis. The cells are usually cultured in Dulbecco's Modified Eagle Medium, with the addition of 20 µM pyridoxal (PL) or pyridoxine (PN) for vitamin B6. The difference between these vitamin B6 compounds is thought not to affect cell proliferation, whereas their influence on other physiological effects is poorly understood. In the present study, the effects of PL and PN on cell proliferation and melanogenesis in B16F10 cells were compared. At 500 µM PL significantly suppressed cell growth but the growth inhibitory effect of PN was weak. Although neither of the vitamin B6 compounds affected cell growth at 20 µM, melanogenesis was suppressed by 20 µM PL compared with the effect of PN. In addition, the expression levels of tyrosinase, which is the rate‑limiting enzyme, correlated with the melanin content. The results of the present study indicate that PL may be more useful for melanoma therapy and suppression of skin pigmentation than PN. The results also signify the importance of medium selection for cell culture.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Matsuo T, Fujiwara A, Nakamura K and Sadzuka Y: The effects of vitamin B6 compounds on cell proliferation and melanogenesis in B16F10 melanoma cells. Oncol Lett 15: 5181-5184, 2018
APA
Matsuo, T., Fujiwara, A., Nakamura , K., & Sadzuka, Y. (2018). The effects of vitamin B6 compounds on cell proliferation and melanogenesis in B16F10 melanoma cells. Oncology Letters, 15, 5181-5184. https://doi.org/10.3892/ol.2018.7947
MLA
Matsuo, T., Fujiwara, A., Nakamura , K., Sadzuka, Y."The effects of vitamin B6 compounds on cell proliferation and melanogenesis in B16F10 melanoma cells". Oncology Letters 15.4 (2018): 5181-5184.
Chicago
Matsuo, T., Fujiwara, A., Nakamura , K., Sadzuka, Y."The effects of vitamin B6 compounds on cell proliferation and melanogenesis in B16F10 melanoma cells". Oncology Letters 15, no. 4 (2018): 5181-5184. https://doi.org/10.3892/ol.2018.7947