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Article

Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells

  • Authors:
    • Ding Shi
    • Yongpan Liu
    • Dong Wu
    • Xujun Hu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Ningbo No. 2 Hospital, Ningbo, Zhejiang 315000, P.R. China, Department of Gastroenterology, First People's Hospital of Yuhang District, Hangzhou, Zhejiang 311100, P.R. China
  • Pages: 5208-5212
    |
    Published online on: February 6, 2018
       https://doi.org/10.3892/ol.2018.7960
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Abstract

Helicobacter pylori, the primary causative agent of stomach cancer, is known to affect gastric mucin expression. However, the underlying molecular mechanisms mediating this H. pylori‑dependent effect remain unknown. In the present study, the effect of exogenous expression of the H. pylori virulence factor, CagA, on mucin 5AC oligomeric muscus/gel‑forming (MUC5AC) expression was investigated using an in vitro model of the gastric mucosa. AGS cells were either untreated or transfected by a vector control (pCDNA3.1) or heterologous DNA, which induced CagA overexpression (pCDNA3.1‑CagA). The expression and functionality of MUC5AC was analyzed using the reverse transcription‑quantitative polymerase chain reaction and immunofluorescence assays. The expression of H. pylori‑CagA in AGS cells was able to significantly upregulate MUC5AC expression compared to the vector control. In addition, immunofluorescence assays were able to validate increased MUC5AC expression following exogenous expression of H. pylori‑CagA. The results of the present study revealed that the H. pylori‑derived virulence factor CagA was able to increase the expression of MUC5AC. As this mucin constitutes an important ecological niche for H. pylori, this response may be involved in H. pylori colonization of the stomach.
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1 

Ho SB, Takamura K, Anway R, Shekels LL, Toribara NW and Ota H: The adherent gastric mucous layer is composed of alternating layers of MUC5AC and MUC6 mucin proteins. Dig Dis Sci. 49:1598–1606. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Byrd JC, Yunker CK, Xu QS, Sternberg LR and Bresalier RS: Inhibition of gastric mucin synthesis by Helicobacter pylori. Gastroenterology. 118:1072–1079. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Hunt RH, Camilleri M, Crowe SE, El-Omar EM, Fox JG, Kuipers EJ, Malfertheiner P, McColl KE, Pritchard DM, Rugge M, et al: The stomach in health and disease. Gut. 64:1650–1668. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Allen A, Newton J, Oliver L, Jordan N, Strugala V, Pearson JP and Dettmar PW: Mucus and H. pylori. J Physiol Pharmacol. 48:297–305. 1997.PubMed/NCBI

5 

van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ and Peppelenbosch MP: Sonic hedgehog regulates gastric gland morphogenesis in man and mouse. Gastroenterology. 121:317–328. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Van de Bovenkamp JH, Mahdavi J, Korteland-Van Male AM, Büller HA, Einerhand AW, Borén T and Dekker J: The MUC5AC glycoprotein is the primary receptor for Helicobacter pylori in the human stomach. Helicobacter. 8:521–532. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Kocer B, Ulas M, Ustundag Y, Erdogan S, Karabeyoglu M, Yldrm O, Unal B, Cengiz O and Soran A: A confirmatory report for the close interaction of Helicobacter pylori with gastric epithelial MUC5AC expression. J Clin Gastroenterol. 38:496–502. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Lindén SK, Wickström C, Lindell G, Gilshenan K and Carlstedt I: Four modes of adhesion are used during Helicobacter pylori binding to human mucins in the oral and gastric niches. Helicobacter. 13:81–93. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Magalhães A and Reis CA: Helicobacter pylori adhesion to gastric epithelial cells is mediated by glycan receptors. Braz J Med Biol Res. 43:611–618. 2010. View Article : Google Scholar : PubMed/NCBI

10 

van Den Brink GR, ten Kate FJ, Ponsioen CY, Rive MM, Tytgat GN, van Deventer SJ and Peppelenbosch MP: Expression and activation of NF-kappa B in the antrum of the human stomach. J Immunol. 164:3353–3359. 2000. View Article : Google Scholar : PubMed/NCBI

11 

Kang HM, Kim N, Park YS, Hwang JH, Kim JW, Jeong SH, Lee DH, Lee HS, Jung HC and Song IS: Effects of Helicobacter pylori Infection on gastric mucin expression. J Clin Gastroenterol. 42:29–35. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Shi D, Qiu XM and Bao YF: Effects of Helicobacter pylori infection on MUC5AC protein expression in gastric cancer. Future Oncol. 9:115–120. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Shi D, Qiu XM and Yan XJ: The changes in MUC5AC expression in gastric cancer before and after Helicobacter pylori eradication. Clin Res Hepatol Gastroenterol. 38:235–240. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wang RQ and Fang DC: Effects of Helicobacter pylori infection on mucin expression in gastric carcinoma and pericancerous tissues. J Gastroenterol Hepatol. 21:425–431. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Matsuda K, Yamauchi K, Matsumoto T, Sano K, Yamaoka Y and Ota H: Quantitative analysis of the effect of Helicobacter pylori on the expressions of SOX2, CDX2, MUC2, MUC5AC, MUC6, TFF1, TFF2 and TFF3 mRNAs in human gastric carcinoma cells. Scand J Gastroenterol. 43:25–33. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Morgenstern S, Koren R, Moss SF, Fraser G, Okon E and Niv Y: Does Helicobacter pylori affect gastric mucin expression? Relationship between gastric antral mucin expression and H. pylori colonization. Eur J Gastroenterol Hepatol. 13:19–23. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Perrais M, Rousseaux C, Ducourouble MP, Courcol R, Vincent P, Jonckheere N and Van Seuningen I: Helicobacter pylori urease and flagellin alter mucin gene expression in human gastric cancer cells. Gastric Cancer. 17:235–246. 2014. View Article : Google Scholar : PubMed/NCBI

18 

McLean MH and El-Omar EM: Genetics of gastric cancer. Nat Rev Gastroenterol Hepatol. 11:664–674. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Zhang BG, Hu L, Zang MD, Wang HX, Zhao W, Li JF, Su LP, Shao Z, Zhao X, Zhu ZG, et al: Helicobacter pylori CagA induces tumor suppressor gene hypermethylation by upregulating DNMT1 via AKT-NFκB pathway in gastric cancer development. Oncotarget. 7:9788–9800. 2016.PubMed/NCBI

20 

Figura N, Marano L, Moretti E and Ponzetto A: Helicobacter pylori infection and gastric carcinoma: Not all the strains and patients are alike. World J Gastrointest Oncol. 8:40–54. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Tohidpour A: CagA-mediated pathogenesis of Helicobacter pylori. Microb Pathog. 93:44–55. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Handa O, Naito Y and Yoshikawa T: CagA protein of Helicobacter pylori: A hijacker of gastric epithelial cell signaling. Biochem Pharmacol. 73:1697–1702. 2007. View Article : Google Scholar : PubMed/NCBI

23 

Tanaka H, Yoshida M and Azuma T: The role of CagA in H. pylori infection. Nihon Rinsho. 67:2245–2249. 2009.(In Japanese).

24 

Stein M, Ruggiero P, Rappuoli R and Bagnoli F: Helicobacter pylori CagA: From pathogenic mechanisms to its use as an anti-cancer vaccine. Front Immunol. 4:3282013. View Article : Google Scholar : PubMed/NCBI

25 

Perrais M, Pigny P, Buisine MP, Porchet N, Aubert JP and Van Seuningen-Lempire I: Aberrant expression of human mucin gene MUC5B in gastric carcinoma and cancer cells. Identification and regulation of a distal promoter. J Biol Chem. 276:15386–15396. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Hayashi T, Senda M, Morohashi H, Higashi H, Horio M, Kashiba Y, Nagase L, Sasaya D, Shimizu T, Venugopalan N, et al: Tertiary structure-function analysis reveals the pathogenic signaling potentiation mechanism of Helicobacter pylori oncogenic effector CagA. Cell Host Microbe. 12:20–33. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Wu J, Xu S and Zhu Y: Helicobacter pylori CagA: A critical destroyer of the gastric epithelial barrier. Dig Dis Sci. 58:1830–1837. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Nordman H, Davies JR, Lindell G, de Bolós C, Real F and Carlstedt I: Gastric MUC5AC and MUC6 are large oligomeric mucins that differ in size, glycosylation and tissue distribution. Biochem J. 364:191–200. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Hatakeyama M: Oncogenic mechanisms of the Helicobacter pylori CagA protein. Nat Rev Cancer. 4:688–694. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Fukuda M, Kawakubo M, Ito Y, Kobayashi M, Lee H and Nakayama J: Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori. Methods Enzymol. 415:164–179. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Kawakubo M, Ito Y, Okimura Y, Kobayashi M, Sakura K, Kasama S, Fukuda MN, Fukuda M and Katsuyama T: Natural antibiotic function of a human gastric mucin against Helicobacter pylori infection. Science. 305:1003–1006. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Kobayashi M, Lee H, Nakayama J and Fukuda M: Roles of gastric mucin-type O-glycans in the pathogenesis of Helicobacter pylori infection. Glycobiology. 19:453–461. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Ota H, Nakayama J, Shimizu T, Nakayama J, Graham DY and Katsuyama T: Relation of H pyroli to gastric mucins and gastric surface mucous gel layer. Gut. 48:869–871. 2001. View Article : Google Scholar : PubMed/NCBI

35 

Tanaka S, Mizuno M, Maga T, Yoshinaga F, Tomoda J, Nasu J, Okada H, Yokota K, Oguma K, Shiratori Y and Tsuji T: H. pylori decreases gastric mucin synthesis via inhibition of galactosyltransferase. Hepatogastroenterology. 50:1739–1742. 2003.PubMed/NCBI

36 

Jia Y, Persson C, Hou L, Zheng Z, Yeager M, Lissowska J, Chanock SJ, Chow WH and Ye W: A comprehensive analysis of common genetic variation in MUC1, MUC5AC, MUC6 genes and risk of stomach cancer. Cancer Causes Control. 21:313–321. 2010. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Shi D, Liu Y, Wu D and Hu X: Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells. Oncol Lett 15: 5208-5212, 2018.
APA
Shi, D., Liu, Y., Wu, D., & Hu, X. (2018). Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells. Oncology Letters, 15, 5208-5212. https://doi.org/10.3892/ol.2018.7960
MLA
Shi, D., Liu, Y., Wu, D., Hu, X."Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells". Oncology Letters 15.4 (2018): 5208-5212.
Chicago
Shi, D., Liu, Y., Wu, D., Hu, X."Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells". Oncology Letters 15, no. 4 (2018): 5208-5212. https://doi.org/10.3892/ol.2018.7960
Copy and paste a formatted citation
x
Spandidos Publications style
Shi D, Liu Y, Wu D and Hu X: Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells. Oncol Lett 15: 5208-5212, 2018.
APA
Shi, D., Liu, Y., Wu, D., & Hu, X. (2018). Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells. Oncology Letters, 15, 5208-5212. https://doi.org/10.3892/ol.2018.7960
MLA
Shi, D., Liu, Y., Wu, D., Hu, X."Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells". Oncology Letters 15.4 (2018): 5208-5212.
Chicago
Shi, D., Liu, Y., Wu, D., Hu, X."Transfection of the Helicobacter pylori CagA gene alters MUC5AC expression in human gastric cancer cells". Oncology Letters 15, no. 4 (2018): 5208-5212. https://doi.org/10.3892/ol.2018.7960
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