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Article

lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway

  • Authors:
    • Yuanyuan Wang
    • Jiaojiao Gu
    • Xiangde Lin
    • Wei Yan
    • Wenchao Yang
    • Guoyang Wu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Zhongshan Hospital, Xiamen University, Xiamen, Fujian 361000, P.R. China, Digestive Diseases Center of Xiamen University Affiliated Zhongshan Hospital, Xiamen, Fujian 361004, P.R. China
  • Pages: 5865-5870
    |
    Published online on: February 9, 2018
       https://doi.org/10.3892/ol.2018.8017
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Abstract

Thyroid cancer is one of the most common types of cancer in the endocrine system. Among all types of thyroid cancer, papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. Long non‑coding RNA (lncRNA) BRAF‑activated non‑protein‑coding RNA (BANCR) is a 688‑bp‑long nucleotide transcript, which was first identified in melanoma. The function of BANCR in thyroid cancer remains unclear. The aim of the present study was to investigate whether BANCR is involved in the development of thyroid cancer. The results indicated that BANCR expression was increased in thyroid tumors compared with in adjacent normal tissues. Among cancer cell lines, the expression level of BANCR differed: BANCR expression in BCPAP cell lines was lower compared with that in CAL‑62, WRO and FTC‑133 cell lines. Overexpression of BANCR promoted the migration and invasion of BCPAP cells. Additionally, BANCR mediated epithelial‑mesenchymal transition (EMT) by regulating the expression of epithelial (E)‑cadherin, vimentin and neuronal (N)‑cadherin. Overexpression of BANCR in BCPAP cells decreased the expression of E‑cadherin and increased the expression of vimentin, N‑cadherin, phospho (p)‑c‑Raf, p‑extracellular‑signal‑regulated kinase (ERK)/mitogen activated protein kinase (MEK)1/2 and p‑ERK1/2. Administration of U0126 inhibitor inhibited the regulation of phosphorylation levels by MEK1/2 and ERK1/2. Additionally, U0126 upregulated the expression of E‑cadherin and downregulated the expression of vimentin. Taken together, the results of the present study suggest that BANCR induces EMT in PTC through the Raf/MEK/ERK signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Gu J, Lin X, Yan W, Yang W and Wu G: lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway. Oncol Lett 15: 5865-5870, 2018.
APA
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., & Wu, G. (2018). lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway. Oncology Letters, 15, 5865-5870. https://doi.org/10.3892/ol.2018.8017
MLA
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., Wu, G."lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway". Oncology Letters 15.4 (2018): 5865-5870.
Chicago
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., Wu, G."lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway". Oncology Letters 15, no. 4 (2018): 5865-5870. https://doi.org/10.3892/ol.2018.8017
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Gu J, Lin X, Yan W, Yang W and Wu G: lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway. Oncol Lett 15: 5865-5870, 2018.
APA
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., & Wu, G. (2018). lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway. Oncology Letters, 15, 5865-5870. https://doi.org/10.3892/ol.2018.8017
MLA
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., Wu, G."lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway". Oncology Letters 15.4 (2018): 5865-5870.
Chicago
Wang, Y., Gu, J., Lin, X., Yan, W., Yang, W., Wu, G."lncRNA BANCR promotes EMT in PTC via the Raf/MEK/ERK signaling pathway". Oncology Letters 15, no. 4 (2018): 5865-5870. https://doi.org/10.3892/ol.2018.8017
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