Open Access

Dynamic quantitative detection of ABC transporter family promoter methylation by MS‑HRM for predicting MDR in pancreatic cancer

  • Authors:
    • Lie Yao
    • Jichun Gu
    • Yishen Mao
    • Xinju Zhang
    • Xiaoyi Wang
    • Chen Jin
    • Deliang Fu
    • Ji Li
  • View Affiliations

  • Published online on: February 13, 2018     https://doi.org/10.3892/ol.2018.8041
  • Pages: 5602-5610
  • Copyright: © Yao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The main focus of the present study was to evaluate whether ABC transporter family promoter methylation predicted multidrug resistance in gemcitabine‑resistant cancer cell lines (BxPC‑3/Gem and PANC‑1/Gem). Using low concentrations of gemcitabine, the cell lines acquired drug resistance with different initial gemcitabine concentrations. A novel technology, methylation‑sensitive high‑resolution melting, was used to monitor the dynamic changes of ABC transporter family promoter methylation, including ATP binding cassette subfamily B member 1 (ABCB1), ATP binding cassette subfamily C (ABCC) and ATP binding cassette subfamily G member 2 (ABCG2) mRNA expression. It was revealed that, with elevation of initial gemcitabine concentration, expression of ABCB1, ABCC and ABCG2 mRNA and corresponding downstream proteins was increased while promoter methylation was decreased. These discoveries indicate that promoter methylation of ABCB1, ABCC and ABCG2 may be a valuable indicator of drug‑resistance characteristics in BxPC‑3/Gem and PANC‑1/Gem cells via quantitative and simultaneous detection. These results also implied that MDR in pancreatic cancer not only arises from gene mutation, but also originates from promoter methylation.
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April-2018
Volume 15 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Yao L, Gu J, Mao Y, Zhang X, Wang X, Jin C, Fu D and Li J: Dynamic quantitative detection of ABC transporter family promoter methylation by MS‑HRM for predicting MDR in pancreatic cancer. Oncol Lett 15: 5602-5610, 2018
APA
Yao, L., Gu, J., Mao, Y., Zhang, X., Wang, X., Jin, C. ... Li, J. (2018). Dynamic quantitative detection of ABC transporter family promoter methylation by MS‑HRM for predicting MDR in pancreatic cancer. Oncology Letters, 15, 5602-5610. https://doi.org/10.3892/ol.2018.8041
MLA
Yao, L., Gu, J., Mao, Y., Zhang, X., Wang, X., Jin, C., Fu, D., Li, J."Dynamic quantitative detection of ABC transporter family promoter methylation by MS‑HRM for predicting MDR in pancreatic cancer". Oncology Letters 15.4 (2018): 5602-5610.
Chicago
Yao, L., Gu, J., Mao, Y., Zhang, X., Wang, X., Jin, C., Fu, D., Li, J."Dynamic quantitative detection of ABC transporter family promoter methylation by MS‑HRM for predicting MDR in pancreatic cancer". Oncology Letters 15, no. 4 (2018): 5602-5610. https://doi.org/10.3892/ol.2018.8041