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Article Open Access

MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study

  • Authors:
    • Rong‑Quan He
    • Xia Yang
    • Liang Liang
    • Gang Chen
    • Jie Ma
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of General Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
    Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5517-5532
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    Published online on: February 13, 2018
       https://doi.org/10.3892/ol.2018.8045
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Abstract

The present study aimed to explore the potential clinical significance of microRNA (miR)-124-3p expression in the hepatocarcinogenesis and development of hepatocellular carcinoma (HCC), as well as the potential target genes of functional HCC pathways. Reverse transcription‑quantitative polymerase chain reaction was performed to evaluate the expression of miR‑124‑3p in 101 HCC and adjacent non‑cancerous tissue samples. Additionally, the association between miR‑124‑3p expression and clinical parameters was also analyzed. Differentially expressed genes identified following miR‑124‑3p transfection, the prospective target genes predicted in silico and the key genes of HCC obtained from Natural Language Processing (NLP) were integrated to obtain potential target genes of miR‑124‑3p in HCC. Relevant signaling pathways were assessed with protein‑protein interaction (PPI) networks, Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Protein Annotation Through Evolutionary Relationships (PANTHER) pathway enrichment analysis. miR‑124‑3p expression was significantly reduced in HCC tissues compared with expression in adjacent non‑cancerous liver tissues. In HCC, miR‑124‑3p was demonstrated to be associated with clinical stage. The mean survival time of the low miR‑124‑3p expression group was reduced compared with that of the high expression group. A total of 132 genes overlapped from differentially expressed genes, miR‑124‑3p predicted target genes and NLP identified genes. PPI network construction revealed a total of 109 nodes and 386 edges, and 20 key genes were identified. The major enriched terms of three GO categories included regulation of cell proliferation, positive regulation of cellular biosynthetic processes, cell leading edge, cytosol and cell projection, protein kinase activity, transcription activator activity and enzyme binding. KEGG analysis revealed pancreatic cancer, prostate cancer and non‑small cell lung cancer as the top three terms. Angiogenesis, the endothelial growth factor receptor signaling pathway and the fibroblast growth factor signaling pathway were identified as the most significant terms in the PANTHER pathway analysis. The present study confirmed that miR‑124‑3p acts as a tumor suppressor in HCC. miR‑124‑3p may target multiple genes, exerting its effect spatiotemporally, or in combination with a diverse range of processes in HCC. Functional characterization of miR‑124‑3p targets will offer novel insight into the molecular changes that occur in HCC progression.
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Copy and paste a formatted citation
Spandidos Publications style
He RQ, Yang X, Liang L, Chen G and Ma J: MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study. Oncol Lett 15: 5517-5532, 2018.
APA
He, R., Yang, X., Liang, L., Chen, G., & Ma, J. (2018). MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study. Oncology Letters, 15, 5517-5532. https://doi.org/10.3892/ol.2018.8045
MLA
He, R., Yang, X., Liang, L., Chen, G., Ma, J."MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study". Oncology Letters 15.4 (2018): 5517-5532.
Chicago
He, R., Yang, X., Liang, L., Chen, G., Ma, J."MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study". Oncology Letters 15, no. 4 (2018): 5517-5532. https://doi.org/10.3892/ol.2018.8045
Copy and paste a formatted citation
x
Spandidos Publications style
He RQ, Yang X, Liang L, Chen G and Ma J: MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study. Oncol Lett 15: 5517-5532, 2018.
APA
He, R., Yang, X., Liang, L., Chen, G., & Ma, J. (2018). MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study. Oncology Letters, 15, 5517-5532. https://doi.org/10.3892/ol.2018.8045
MLA
He, R., Yang, X., Liang, L., Chen, G., Ma, J."MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study". Oncology Letters 15.4 (2018): 5517-5532.
Chicago
He, R., Yang, X., Liang, L., Chen, G., Ma, J."MicroRNA‑124‑3p expression and its prospective functional pathways in hepatocellular carcinoma: A quantitative polymerase chain reaction, gene expression omnibus and bioinformatics study". Oncology Letters 15, no. 4 (2018): 5517-5532. https://doi.org/10.3892/ol.2018.8045
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