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Article Open Access

CD44 promotes cell proliferation in non-small cell lung cancer

  • Authors:
    • Bo Hu
    • Yuanyuan Ma
    • Yue Yang
    • Lijian Zhang
    • Haibo Han
    • Jinfeng Chen
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery II, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China, Department of Biobank, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Peking University Cancer Hospital and Institute, Beijing 100142, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5627-5633
    |
    Published online on: February 14, 2018
       https://doi.org/10.3892/ol.2018.8051
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Abstract

Cluster of differentiation 44 (CD44), a marker for cancer stem cells, has been reported to be associated with poor prognosis in non‑small cell lung cancer (NSCLC), but its involvement in tumor growth has not fully been elucidated. The present study explored the associations between CD44 expression and clinicopathological features using immunohistochemistry in 94 NSCLC cases, as well as proliferation in cells with aberrant expression of this protein. Overexpression of CD44 was achieved by transfecting H1299 cells with CD44 expression vectors, and inhibition of CD44 expression was performed by transfecting small interfering RNAs into A549 cells. Cell proliferation was measured using Cell Counting Kit‑8 assays and flat plate colony formation assay was performed to confirm the cellular anchorage growth in vitro. In total, 64 (68.1%) of the 94 NSCLC cases stained positive for CD44 expression. High expression of CD44 was associated with advanced T stage in NSCLC. Overexpression of CD44 in H1299 cells promoted cell proliferation and colony formation in vitro. Meanwhile, knockdown of CD44 expression in A549 cells suppressed cell proliferation and colony formation in vitro. High expression of CD44 may promote NSCLC progression by increasing cancer cell proliferation; therefore, it may serve as a potential biomarker for diagnosis or target therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Hu B, Ma Y, Yang Y, Zhang L, Han H and Chen J: CD44 promotes cell proliferation in non-small cell lung cancer. Oncol Lett 15: 5627-5633, 2018.
APA
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., & Chen, J. (2018). CD44 promotes cell proliferation in non-small cell lung cancer. Oncology Letters, 15, 5627-5633. https://doi.org/10.3892/ol.2018.8051
MLA
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., Chen, J."CD44 promotes cell proliferation in non-small cell lung cancer". Oncology Letters 15.4 (2018): 5627-5633.
Chicago
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., Chen, J."CD44 promotes cell proliferation in non-small cell lung cancer". Oncology Letters 15, no. 4 (2018): 5627-5633. https://doi.org/10.3892/ol.2018.8051
Copy and paste a formatted citation
x
Spandidos Publications style
Hu B, Ma Y, Yang Y, Zhang L, Han H and Chen J: CD44 promotes cell proliferation in non-small cell lung cancer. Oncol Lett 15: 5627-5633, 2018.
APA
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., & Chen, J. (2018). CD44 promotes cell proliferation in non-small cell lung cancer. Oncology Letters, 15, 5627-5633. https://doi.org/10.3892/ol.2018.8051
MLA
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., Chen, J."CD44 promotes cell proliferation in non-small cell lung cancer". Oncology Letters 15.4 (2018): 5627-5633.
Chicago
Hu, B., Ma, Y., Yang, Y., Zhang, L., Han, H., Chen, J."CD44 promotes cell proliferation in non-small cell lung cancer". Oncology Letters 15, no. 4 (2018): 5627-5633. https://doi.org/10.3892/ol.2018.8051
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