Open Access

TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells

  • Authors:
    • Peifen Zheng
    • Weifeng Wang
    • Muxi Ji
    • Qin Zhu
    • Yuliang Feng
    • Feng Zhou
    • Qiaona He
  • View Affiliations

  • Published online on: March 28, 2018     https://doi.org/10.3892/ol.2018.8358
  • Pages: 8281-8286
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Abstract

Gastric cancer is the second major cause of death associated with cancer and ranks among the top four cancers diagnosed worldwide. Previous findings identified the association of transmembrane proteins (TMEMs) with tumorigenesis of various types of cancer, including breast, liver and kidney cancer. However, the expression and the biological function of TMEMs, especially TMEM119, and its possible molecular mechanism in gastric cancer remain less understood. CCK-8 and flow cytometric analysis was employed to examine the viability and apoptosis of gastric adenocarcinoma SGC-7901 and AGS cells, gastric carcinoma MKN45 cells, as well as gastric epithelial cell lines GES-1 after transfection with TMEM119-siRNA (siTMEM119), respectively. Quantitative PCR, western blot analysis and immunohistochemistry was performed to detect the expression levels of TMEM119, Bax, Bcl-2 and caspase-3. The results showed that, TMEM119 was elevated with the highest expression detected in SGC-7901 cells compared to AGS cells, MKN45 cells, as well as GES-1. TMEM119 silencing in the gastric cancer cell line, SGC-7901, significantly inhibited cell viability and induced apoptosis. The downregulation of TMEM119 exhibited reduced levels of Bcl-2 and higher levels of Bax and caspase-3 in SGC-7901 cells. These results suggest that TMEM119 is useful in the treatment of gastric cancer.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zheng P, Wang W, Ji M, Zhu Q, Feng Y, Zhou F and He Q: TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells. Oncol Lett 15: 8281-8286, 2018
APA
Zheng, P., Wang, W., Ji, M., Zhu, Q., Feng, Y., Zhou, F., & He, Q. (2018). TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells. Oncology Letters, 15, 8281-8286. https://doi.org/10.3892/ol.2018.8358
MLA
Zheng, P., Wang, W., Ji, M., Zhu, Q., Feng, Y., Zhou, F., He, Q."TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells". Oncology Letters 15.6 (2018): 8281-8286.
Chicago
Zheng, P., Wang, W., Ji, M., Zhu, Q., Feng, Y., Zhou, F., He, Q."TMEM119 silencing inhibits cell viability and causes the apoptosis of gastric cancer SGC-7901 cells". Oncology Letters 15, no. 6 (2018): 8281-8286. https://doi.org/10.3892/ol.2018.8358