Open Access

miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1

  • Authors:
    • Jianyong Liu
    • Bo Mi
    • Yi Wang
    • Chunling Shi
    • Xiufang Mi
    • Yingying Lu
    • Peilin Yu
  • View Affiliations

  • Published online on: March 28, 2018     https://doi.org/10.3892/ol.2018.8359
  • Pages: 8303-8310
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Abstract

Osteosarcoma (OS) is identified as the most commonly diagnosed malignant cancer of bone, and has approximately three million new cases annually. miR-26a plays an important role in the development of various types of cancer. We investigated whether miR-26a can regulate the migration and invasion of OS by targeting high-mobility group A1 HMGA1. Western blot analysis was used to identify the changes of protein levels. Reverse transcription‑quantitative PCR was used to test expression levels of genes and miR-26a. Luciferase reporter assay was used to test the specific target gene of miR-26a. Transwell assay was employed to determine the migration and invasion of OS cell lines. In the present study, miRNA-26a was frequently downregulated in OS tissues and cells. Overexpression of miR-26a inhibited cell migration and invasion in vitro. In addition, miR-26a downregulated HMGA1 by targeting its 3'-UTR and knockdown of HMGA1 significantly suppressed the migration and invasion of two osteosarcoma cell lines in vitro. miR-26a suppressed the migration and invasion of OS cells by targeting HMGA1, suggesting that miR-26a/HMGA1 axis provides a new prospective therapeutic strategy for OS.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu J, Mi B, Wang Y, Shi C, Mi X, Lu Y and Yu P: miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncol Lett 15: 8303-8310, 2018
APA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., & Yu, P. (2018). miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncology Letters, 15, 8303-8310. https://doi.org/10.3892/ol.2018.8359
MLA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15.6 (2018): 8303-8310.
Chicago
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15, no. 6 (2018): 8303-8310. https://doi.org/10.3892/ol.2018.8359