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miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1

  • Authors:
    • Jianyong Liu
    • Bo Mi
    • Yi Wang
    • Chunling Shi
    • Xiufang Mi
    • Yingying Lu
    • Peilin Yu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, People's Hospital of Weifang, Weifang, Shandong 261000, P.R. China, Department of Medicine, The First People's Hospital of Jinan, Jinan, Shandong 250000, P.R. China, Department of Respiratory Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Neurology, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, Department of Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Clinical Laboratory, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China, Department of Orthopedics, Qingdao Hiser Medical Center, Qingdao, Shandong 266033, P.R. China
  • Pages: 8303-8310
    |
    Published online on: March 28, 2018
       https://doi.org/10.3892/ol.2018.8359
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Abstract

Osteosarcoma (OS) is identified as the most commonly diagnosed malignant cancer of bone, and has approximately three million new cases annually. miR-26a plays an important role in the development of various types of cancer. We investigated whether miR-26a can regulate the migration and invasion of OS by targeting high-mobility group A1 HMGA1. Western blot analysis was used to identify the changes of protein levels. Reverse transcription‑quantitative PCR was used to test expression levels of genes and miR-26a. Luciferase reporter assay was used to test the specific target gene of miR-26a. Transwell assay was employed to determine the migration and invasion of OS cell lines. In the present study, miRNA-26a was frequently downregulated in OS tissues and cells. Overexpression of miR-26a inhibited cell migration and invasion in vitro. In addition, miR-26a downregulated HMGA1 by targeting its 3'-UTR and knockdown of HMGA1 significantly suppressed the migration and invasion of two osteosarcoma cell lines in vitro. miR-26a suppressed the migration and invasion of OS cells by targeting HMGA1, suggesting that miR-26a/HMGA1 axis provides a new prospective therapeutic strategy for OS.
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Copy and paste a formatted citation
Spandidos Publications style
Liu J, Mi B, Wang Y, Shi C, Mi X, Lu Y and Yu P: miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncol Lett 15: 8303-8310, 2018.
APA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., & Yu, P. (2018). miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncology Letters, 15, 8303-8310. https://doi.org/10.3892/ol.2018.8359
MLA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15.6 (2018): 8303-8310.
Chicago
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15, no. 6 (2018): 8303-8310. https://doi.org/10.3892/ol.2018.8359
Copy and paste a formatted citation
x
Spandidos Publications style
Liu J, Mi B, Wang Y, Shi C, Mi X, Lu Y and Yu P: miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncol Lett 15: 8303-8310, 2018.
APA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., & Yu, P. (2018). miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1. Oncology Letters, 15, 8303-8310. https://doi.org/10.3892/ol.2018.8359
MLA
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15.6 (2018): 8303-8310.
Chicago
Liu, J., Mi, B., Wang, Y., Shi, C., Mi, X., Lu, Y., Yu, P."miR-26a suppresses osteosarcoma migration and invasion by directly targeting HMGA1". Oncology Letters 15, no. 6 (2018): 8303-8310. https://doi.org/10.3892/ol.2018.8359
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