Identification and functional characterization of long non-coding RNAs in human gastric cancer

Li,Cheng‑Yun; Liang,Ge‑Yu; Yao,Wen‑Zhuo; Sui,Jing; Shen,Xian; Zhang,Yan‑Qiu; Ma,Shu‑Mei; Ye,Yan‑Cheng; Zhang,Zhi‑Yi; Zhang,Wen‑Hua; Yin,Li‑Hong; Pu,Yue‑Pu

doi:10.3892/ol.2018.8369

Identification and functional characterization of long non-coding RNAs in human gastric cancer

Authors:
- Cheng‑Yun Li
- Ge‑Yu Liang
- Wen‑Zhuo Yao
- Jing Sui
- Xian Shen
- Yan‑Qiu Zhang
- Shu‑Mei Ma
- Yan‑Cheng Ye
- Zhi‑Yi Zhang
- Wen‑Hua Zhang
- Li‑Hong Yin
- Yue‑Pu Pu
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Affiliations: Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Cancer Epidemiology, Wuwei Cancer Registry, Gansu Wuwei Tumor Hospital, Wuwei, Gansu 733000, P.R. China
Published online on: March 28, 2018 https://doi.org/10.3892/ol.2018.8369
Pages: 8805-8815

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Abstract

Abnormal regulation of long non‑coding RNAs (lncRNAs) appears to be a primary feature of numerous types of human cancer. However, the association between the dysregulation of lncRNAs and functional alterations in gastric cancer (GC) remains unclear. In previous studies, we applied microarray and bioinformatics analyses to screen for key lncRNAs from the tumor tissues and matched adjacent non‑tumor tissues of 10 patients with GC. There were seven key lncRNAs demonstrated to be significantly different between carcinoma tissues and adjacent non‑tumor tissues. In the present study, the expression of these seven selected lncRNAs were validated in 82 patients with GC to further investigate the association between lncRNAs and GC clinical characterization. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) results demonstrated that RP5‑919F19, MCPH1 antisense RNA 1 (CTD‑2541M15) and urothelial carcinoma‑associated 1 (UCA1) exhibited consistent upregulation in cancer compared with adjacent non‑tumor tissues, whereas AP000459, LOC101928316, tumor suppressor candidate 8 (LINC01071) and maternally expressed 3 (MEG3) showed consistent downregulation. The results from the microarray and RT‑qPCR experiments achieved 100% agreement. A correlation analysis indicated that RP5‑919F19, LOC101928316 and MEG3 were significantly associated with tumor differentiation degree, RP5‑919F19, UCA1 and MEG3 were significantly associated with lymph node metastasis, and RP5‑919F19, CTD‑2541M15 and UCA1 were significantly associated with tumor‑node‑metastasis stage (P<0.05). In addition, it was identified that the differential expression of LINC01071 and LOC101928316 significantly correlated with the age and gender of the GC patients, respectively (P<0.05). The results suggest that the lncRNAs RP5‑919F19, LOC101928316, CTD‑2541M15, UCA1 and MEG3 are closely associated with the invasion and metastasis of GC, which reveals these indicators as potential specificity biomarkers for the diagnosis, prognosis and classification of GC. Thus, these lncRNAs merit further study as novel candidate biomarkers for the clinical diagnosis of GC and as potential targets for therapy.

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