Open Access

Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression

  • Authors:
    • Yadong Wang
    • Teng Pan
    • Li Li
    • Haiyu Wang
    • Ding Zhang
    • Haiyan Yang
  • View Affiliations

  • Published online on: March 29, 2018     https://doi.org/10.3892/ol.2018.8379
  • Pages: 8325-8332
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Benzo(a)pyrene (BaP), a carcinogenic component of cigarette smoke, has been reported to activate extracellular signal‑regulated kinase (ERK) in cancer cells. Furthermore, activated ERK is associated with liver cancer cell invasion and metastasis. Therefore, the aim of the present study was to investigate the potential role of phosphorylated (p)‑ERK in BaP‑induced Hep‑G2 cell migration and invasion. An MTT assay was used to determine the effects of BaP treatment on Hep‑G2 cell proliferation. Wound‑healing and Transwell invasion assays were employed to assess the migration and invasion abilities of Hep‑G2 cells. Western blot analysis was applied to detect the expression of proteins. The results of the present study demonstrated that BaP treatment was able to increase the level of p‑ERK protein expression in Hep‑G2 cells. BaP treatment promoted Hep‑G2 cell migration and invasion. The ERK inhibitor, U0126, was able to block the migration and invasion abilities of Hep‑G2 cells induced by BaP. The results of the present study demonstrated that BaP treatment promoted the migration and invasion of Hep‑G2 cells by upregulating p‑ERK expression.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang Y, Pan T, Li L, Wang H, Zhang D and Yang H: Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression. Oncol Lett 15: 8325-8332, 2018
APA
Wang, Y., Pan, T., Li, L., Wang, H., Zhang, D., & Yang, H. (2018). Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression. Oncology Letters, 15, 8325-8332. https://doi.org/10.3892/ol.2018.8379
MLA
Wang, Y., Pan, T., Li, L., Wang, H., Zhang, D., Yang, H."Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression". Oncology Letters 15.6 (2018): 8325-8332.
Chicago
Wang, Y., Pan, T., Li, L., Wang, H., Zhang, D., Yang, H."Benzo(a)pyrene promotes Hep‑G2 cell migration and invasion by upregulating phosphorylated extracellular signal‑regulated kinase expression". Oncology Letters 15, no. 6 (2018): 8325-8332. https://doi.org/10.3892/ol.2018.8379