Open Access

Knockdown of stromal interaction molecule 1 inhibits proliferation of colorectal cancer cells by inducing apoptosis

  • Authors:
    • Dong Yang
    • Xiaoyu Dai
    • Keqiang Li
    • Yangyang Xie
    • Jianpei Zhao
    • Mingjun Dong
    • Hua Yu
    • Zhenfang Kong
  • View Affiliations

  • Published online on: April 5, 2018     https://doi.org/10.3892/ol.2018.8437
  • Pages: 8231-8236
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Stromal interaction molecule 1 (STIM1) is an endoplasmic reticulum Ca2+ sensor which has been reported to be overexpressed in numerous types of cancer, and is involved in the cell proliferation, invasion, migration and metastasis frequently observed in cancer. However, the role of STIM1 in colorectal cancer (CRC) remains unknown. The purpose of the present study was to investigate the effect of STIM1 in human CRC. The expression of STIM1 was specifically knocked down using lentivirus‑mediated small hairpin RNA (shRNA) interference techniques in the CRC cell lines HCT116 and SW1116. Subsequently, the efficiency of infection was confirmed using green fluorescent protein (GFP)‑positive signals. The knockdown efficiency was further determined using the reverse transcription‑quantitative polymerase chain reaction and western blotting analysis. As a result, CRC cell lines with STIM1 silenced were successfully constructed and subsequently employed in a series of cell function assays. Knockdown of STIM1 significantly suppressed cell proliferation and colony formation, as revealed by an MTT and colony formation assay. Furthermore, it was identified that STIM1 silencing may promote cell apoptosis through the induction of mitochondria‑associated apoptosis, as was identified by increased expression levels of B‑cell lymphoma 2 (Bcl‑2)‑associated death promoter, Bcl‑2‑associated X protein and poly(ADP‑ribose) polymerase cleavage. Therefore, STIM1 may serve a critical role in the progression of CRC by regulating cell proliferation and apoptosis, which may provide a potential therapeutic target for the treatment of CRC.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Yang D, Dai X, Li K, Xie Y, Zhao J, Dong M, Yu H and Kong Z: Knockdown of stromal interaction molecule 1 inhibits proliferation of colorectal cancer cells by inducing apoptosis. Oncol Lett 15: 8231-8236, 2018
APA
Yang, D., Dai, X., Li, K., Xie, Y., Zhao, J., Dong, M. ... Kong, Z. (2018). Knockdown of stromal interaction molecule 1 inhibits proliferation of colorectal cancer cells by inducing apoptosis. Oncology Letters, 15, 8231-8236. https://doi.org/10.3892/ol.2018.8437
MLA
Yang, D., Dai, X., Li, K., Xie, Y., Zhao, J., Dong, M., Yu, H., Kong, Z."Knockdown of stromal interaction molecule 1 inhibits proliferation of colorectal cancer cells by inducing apoptosis". Oncology Letters 15.6 (2018): 8231-8236.
Chicago
Yang, D., Dai, X., Li, K., Xie, Y., Zhao, J., Dong, M., Yu, H., Kong, Z."Knockdown of stromal interaction molecule 1 inhibits proliferation of colorectal cancer cells by inducing apoptosis". Oncology Letters 15, no. 6 (2018): 8231-8236. https://doi.org/10.3892/ol.2018.8437