Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells

  • Authors:
    • Dongjie Liu
    • Rui Li
    • Xianghua Guo
    • Lijun Pang
    • Yunjin Zang
    • Kai Liu
    • Dexi Chen
  • View Affiliations / Copyright

    Affiliations: Capital Medical University Affiliated to Beijing You An Hospital, Beijing 100069, P.R. China, Department of Acupuncture and Mini‑invasive Oncology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing 100029, P.R. China
  • Pages: 9333-9338
    |
    Published online on: April 10, 2018
       https://doi.org/10.3892/ol.2018.8453
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Overexpression of apoptosis‑stimulating of p53 protein 2 (ASPP2) can induce apoptotic cell death in hepatoma cells, which contributes to a killing effect of ASPP2 on treating hepatocellular carcinoma (HCC). In the present study, ASPP2 overexpression failed to induce apoptotic cell death in the HCC Huh7.5 cell line, but promoted autophagy development by inhibiting AKT/mTOR pathway. Inhibition of autophagy using 3‑methyladenosine recovered the function of ASPP2 on inducing apoptotic cell death, indicating that ASPP2‑induced autophagy has an anti‑apoptotic role in Huh7.5 cells. A previous study demonstrated that ASPP2‑induced autophagy could induce apoptosis in a CHOP‑ and DRAM‑dependent manner, in which CHOP is involved in the initiation of autophagy and DRAM allows autophagy to induce apoptosis. In the present study, CHOP and DRAM were not involved in ASPP2‑induced autophagy; however, the induction of DRAM overexpression recovered the apoptosis‑inducing function of ASPP2, indicating that DRAM overexpression switches the role of ASPP2‑induced autophagy from anti‑apoptotic to pro‑apoptotic in Huh7.5 cells. Thus, in combination with DRAM, ASPP2 may better perform its pro‑apoptotic role by preventing the occurrence of anti‑apoptotic autophagy.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Bergamaschi D, Samuels Y, O'Neil NJ, Trigiante G, Crook T, Hsieh JK, O'Connor DJ, Zhong S, Campargue I, Tomlinson ML, et al: iASPP oncoprotein is a key inhibitor of p53 conserved from worm to human. Nat Genet. 33:162–167. 2003. View Article : Google Scholar : PubMed/NCBI

2 

Song B, Bian Q, Zhang YJ, Shao CH, Li G, Liu AA, Jing W, Liu R, Zhou YQ, Jin G and Hu XG: Downregulation of ASPP2 in pancreatic cancer cells contributes to increased resistance to gemcitabine through autophagy activation. Mol Cancer. 14:1772015. View Article : Google Scholar : PubMed/NCBI

3 

Liu K, Jiang T, Ouyang Y, Shi Y, Zang Y, Li N, Lu S and Chen D: Nuclear EGFR impairs ASPP2-p53 complex-induced apoptosis by inducing SOS1 expression in hepatocellular carcinoma. Oncotarget. 6:16507–16516. 2015.PubMed/NCBI

4 

Liu K, Shi Y, Guo X, Wang S, Ouyang Y, Hao M, Liu D, Qiao L, Li N, Zheng J and Chen D: CHOP mediates ASPP2-induced autophagic apoptosis in hepatoma cells by releasing Beclin-1 from Bcl-2 and inducing nuclear translocation of Bcl-2. Cell Death Dis. 5:e13232014. View Article : Google Scholar : PubMed/NCBI

5 

Shirakabe A, Ikeda Y, Sciarretta S, Zablocki DK and Sadoshima J: Aging and autophagy in the heart. Circ Res. 118:1563–1576. 2016. View Article : Google Scholar : PubMed/NCBI

6 

Lin L and Baehrecke EH: Autophagy, cell death, and cancer. Mol Cell Oncol. 2:e9859132015. View Article : Google Scholar : PubMed/NCBI

7 

Liu K, Lou J, Wen T, Yin J, Xu B, Ding W, Wang A, Liu D, Zhang C, Chen D and Li N: Depending on the stage of hepatosteatosis, p53 causes apoptosis primarily through either DRAM-induced autophagy or BAX. Liver Int. 33:1566–1574. 2013.PubMed/NCBI

8 

Tan VP and Miyamoto S: Nutrient-sensing mTORC1: Integration of metabolic and autophagic signals. J Mol Cell Cardiol. 95:31–41. 2016. View Article : Google Scholar : PubMed/NCBI

9 

Zhao J, Wu G, Bu F, Lu B, Liang A, Cao L, Tong X, Lu X, Wu M and Guo Y: Epigenetic silence of ankyrin-repeat-containing, SH3-domain-containing, and proline-rich-region-containing protein 1 (ASPP1) and ASPP2 genes promotes tumor growth in hepatitis B virus-positive hepatocellular carcinoma. Hepatology. 51:142–153. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Wang Y, Wang XD, Lapi E, Sullivan A, Jia W, He YW, Ratnayaka I, Zhong S, Goldin RD, Goemans CG, et al: Autophagic activity dictates the cellular response to oncogenic RAS. Proc Natl Acad Sci USA. 109:13325–13330. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Chen R, Wang H, Liang B, Liu G, Tang M, Jia R, Fan X, Jing W, Zhou X, Wang H, et al: Downregulation of ASPP2 improves hepatocellular carcinoma cells survival via promoting BECN1-dependent autophagy initiation. Cell Death Dis. 7:e25122016. View Article : Google Scholar : PubMed/NCBI

12 

Liu K, Shi Y, Guo XH, Ouyang YB, Wang SS, Liu DJ, Wang AN, Li N and Chen DX: Phosphorylated AKT inhibits the apoptosis induced by DRAM-mediated mitophagy in hepatocellular carcinoma by preventing the translocation of DRAM to mitochondria. Cell Death Dis. 5:e10782014. View Article : Google Scholar : PubMed/NCBI

13 

Flores-Toro JA, Go KL, Leeuwenburgh C and Kim JS: Autophagy in the liver: Cell's cannibalism and beyond. Arch Pharm Res. 39:1050–1061. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Rodríguez ME, Cogno IS, Sanabria Milla LS, Morán YS and Rivarola VA: Heat shock proteins in the context of photodynamic therapy: Autophagy, apoptosis and immunogenic cell death. Photochem Photobiol Sci. 15:1090–1102. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Chagin AS: Effectors of mTOR-autophagy pathway: Targeting cancer, affecting the skeleton. Curr Opin Pharmacol. 28:1–7. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Xu L and Brink M: mTOR, cardiomyocytes and inflammation in cardiac hypertrophy. Biochim Biophys Acta. 1863:1894–1903. 2016. View Article : Google Scholar : PubMed/NCBI

17 

Zheng XT, Wu ZH, Wei Y, Dai JJ, Yu GF, Yuan F and Ye LC: Induction of autophagy by salidroside through the AMPK-mTOR pathway protects vascular endothelial cells from oxidative stress-induced apoptosis. Mol Cell Biochem. 425:125–138. 2017. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liu D, Li R, Guo X, Pang L, Zang Y, Liu K and Chen D: DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells. Oncol Lett 15: 9333-9338, 2018.
APA
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., & Chen, D. (2018). DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells. Oncology Letters, 15, 9333-9338. https://doi.org/10.3892/ol.2018.8453
MLA
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., Chen, D."DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells". Oncology Letters 15.6 (2018): 9333-9338.
Chicago
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., Chen, D."DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells". Oncology Letters 15, no. 6 (2018): 9333-9338. https://doi.org/10.3892/ol.2018.8453
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, Li R, Guo X, Pang L, Zang Y, Liu K and Chen D: DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells. Oncol Lett 15: 9333-9338, 2018.
APA
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., & Chen, D. (2018). DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells. Oncology Letters, 15, 9333-9338. https://doi.org/10.3892/ol.2018.8453
MLA
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., Chen, D."DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells". Oncology Letters 15.6 (2018): 9333-9338.
Chicago
Liu, D., Li, R., Guo, X., Pang, L., Zang, Y., Liu, K., Chen, D."DNA damage regulated autophagy modulator 1 recovers the function of apoptosis‑stimulating of p53 protein 2 on inducing apoptotic cell death in Huh7.5 cells". Oncology Letters 15, no. 6 (2018): 9333-9338. https://doi.org/10.3892/ol.2018.8453
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team