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Article Open Access

PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo

  • Authors:
    • Yang Zhang
    • Zhengrong Li
    • Xiaole Fan
    • Jianbo Xiong
    • Guoyang Zhang
    • Xianshi Luo
    • Kun Li
    • Zhigang Jie
    • Yi Cao
    • Zuoxi Huang
    • Feng Wu
    • Lin Xiao
    • Guangling Duan
    • Heping Chen
  • View Affiliations / Copyright

    Affiliations: Department of Gastrointestinal Surgery, The First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi 330000, P.R. China, The Key Laboratory of Basic Pharmacology, School of Pharmaceutical Science, Nanchang University, Nanchang, Jiangxi 330006, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9069-9074
    |
    Published online on: April 11, 2018
       https://doi.org/10.3892/ol.2018.8467
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Abstract

The peritoneal metastasis‑associated phosphatase of regenerating liver‑3 (PRL‑3) is upregulated in gastric cancer. The phosphatidylinositol 3‑kinase (PI3K)/RAC serine/threonine‑protein kinase (AKT) signaling pathway acts downstream of PRL‑3 in gastric cancer. However, the exact PRL‑3 signaling mechanisms are poorly understood. The present study investigated whether PRL‑3 facilitates the peritoneal metastasis of gastric cancer via the PI3K/AKT pathway in vivo and in vitro. Nude mouse models of peritoneal metastasis were established using SGC7901/PRL‑3 cell lines. The results confirmed that the invasion and migration abilities of SGC7901/PRL‑3 cells were significantly increased in these models. Furthermore, western blotting demonstrated that the expression of p‑AKT, matrix metallopeptidase‑2 (MMP‑2) and ‑9 proteins increased in SGC7901/PRL‑3 cells. These effects were suppressed in SGC7901 cell lines when PI3K was inhibited by LY294002. Furthermore, tumors derived from the peritoneal injection of SGC7901/PRL‑3 cells were significantly smaller when the cells were grown in the presence of LY249002, compared with cells grown in its absence. These results indicated that targeted inhibition of the PI3K/AKT signaling pathway decreased the effects of PRL‑3 on metastasis in vivo. Collectively, the results of the present study indicated that PRL‑3 acts via the PI3K/AKT pathway to promote peritoneal metastasis and invasion of gastric cancer cells in vitro and in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Li Z, Fan X, Xiong J, Zhang G, Luo X, Li K, Jie Z, Cao Y, Huang Z, Huang Z, et al: PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo. Oncol Lett 15: 9069-9074, 2018.
APA
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X. ... Chen, H. (2018). PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo. Oncology Letters, 15, 9069-9074. https://doi.org/10.3892/ol.2018.8467
MLA
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X., Li, K., Jie, Z., Cao, Y., Huang, Z., Wu, F., Xiao, L., Duan, G., Chen, H."PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo". Oncology Letters 15.6 (2018): 9069-9074.
Chicago
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X., Li, K., Jie, Z., Cao, Y., Huang, Z., Wu, F., Xiao, L., Duan, G., Chen, H."PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo". Oncology Letters 15, no. 6 (2018): 9069-9074. https://doi.org/10.3892/ol.2018.8467
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Li Z, Fan X, Xiong J, Zhang G, Luo X, Li K, Jie Z, Cao Y, Huang Z, Huang Z, et al: PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo. Oncol Lett 15: 9069-9074, 2018.
APA
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X. ... Chen, H. (2018). PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo. Oncology Letters, 15, 9069-9074. https://doi.org/10.3892/ol.2018.8467
MLA
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X., Li, K., Jie, Z., Cao, Y., Huang, Z., Wu, F., Xiao, L., Duan, G., Chen, H."PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo". Oncology Letters 15.6 (2018): 9069-9074.
Chicago
Zhang, Y., Li, Z., Fan, X., Xiong, J., Zhang, G., Luo, X., Li, K., Jie, Z., Cao, Y., Huang, Z., Wu, F., Xiao, L., Duan, G., Chen, H."PRL‑3 promotes gastric cancer peritoneal metastasis via the PI3K/AKT signaling pathway in vitro and in vivo". Oncology Letters 15, no. 6 (2018): 9069-9074. https://doi.org/10.3892/ol.2018.8467
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