Phosphoglycerate mutase 1 is highly expressed in C6 glioma cells and human astrocytoma

Liu,Zhi‑Guo; Ding,Jie; Du,Chuan; Xu,Ning; Wang,En‑Le; Li,Jian‑Yi; Wang,Yun‑Yan; Yu,Jin‑Ming

doi:10.3892/ol.2018.8477

Phosphoglycerate mutase 1 is highly expressed in C6 glioma cells and human astrocytoma

Authors:
- Zhi‑Guo Liu
- Jie Ding
- Chuan Du
- Ning Xu
- En‑Le Wang
- Jian‑Yi Li
- Yun‑Yan Wang
- Jin‑Ming Yu
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Affiliations: Department of Neurosurgery, Shandong Cancer Hospital, Shandong Provincial Institute of Cancer Prevention and Treatment, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250017, P.R. China, Department of Respiration Medicine, Xi'an Chest Hospital, Xi'an, Shanxi 710100, P.R. China, Department of Neurosurgery, Zhangqiu People's Hospital of Shandong Province, Jinan, Shandong 250200, P.R. China, Department of Radiology, Zhangqiu People's Hospital of Shandong Province, Jinan, Shandong 250200, P.R. China, Department of Chinese Acupuncture, Zhangqiu People's Hospital of Shandong Province, Jinan, Shandong 250200, P.R. China, Department of Pathology and Laboratory Medicine, North Shore University Hospital and Long Island Jewish Medical Center, Northwell Health, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Lake Success, NY 11042, USA, Department of Neurosurgery, Qilu Hospital of Shandong University, Brain Science Research Institute of Shandong University, Jinan, Shandong 250117, P.R. China, Department of Oncology, Shandong Cancer Hospital, Shandong Provincial Institute of Cancer Prevention and Treatment, Jinan, Shandong 250117, P.R. China
Published online on: April 12, 2018 https://doi.org/10.3892/ol.2018.8477
Pages: 8935-8940
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the expression of phosphoglycerate mutase 1 (PGAM1) in astrocytomas, and to investigate its role in the progression of astrocytomas. The expression of PGAM1 mRNA in rat C6 glioma cells and normal astrocytes was determined using the reverse transcription‑semi‑quantitative polymerase chain reaction, and immunohistochemistry was used to detect the expression of PGAM1 protein in human astrocytomas and adjacent brain tissue. These data suggested that the expression of PGAM1 in rat C6 glioma cells was significantly increased compared with that of normal astrocytes (P<0.05), and the expression of PGAM1 protein in human astrocytoma tissue was significantly increased compared with that of the brain tissue surrounding the tumor (P<0.05). In addition, PGAM1 protein was more frequently expressed in high‑grade astrocytomas compared with low‑grade astrocytomas. These data indicate that the expression of PGAM1 is increased in C6 cells and human astrocytomas, and PGAM1 is probably involved in the tumorigenesis and progression of glioma, which may be a potential target for glioma treatment.

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