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Article

Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8

  • Authors:
    • Ping Lin
    • Lijuan Zhu
    • Wenjing Sun
    • Zhengkai Yang
    • Hui Sun
    • Dong Li
    • Rongjun Cui
    • Xiulan Zheng
    • Xiaoguang Yu
  • View Affiliations / Copyright

    Affiliations: Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China
  • Pages: 9436-9442
    |
    Published online on: April 16, 2018
       https://doi.org/10.3892/ol.2018.8505
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Abstract

MicroRNAs (miRNAs/miRs), which are endogenous non‑coding single‑stranded RNAs 19‑25 nucleotides in length, regulate gene expression by blocking translation or transcription repression. The present study revealed that miR‑3160‑5p was widely expressed in prostate cancer cells by reverse transcription‑quantitative polymerase chain reaction. There was a negative association between the expression of miR‑3160‑5p and F‑box and WD repeat domain containing 8 (Fbxw8) in prostate cancer DU145 cells. A luciferase activity assay was used to verify that Fbxw8 is the target of miR‑3160‑5p. In the present study, using MTT assay and cell cycle analysis, it was demonstrated that DU145 cell proliferation was repressed and the cell cycle was arrested in the G2/M cell cycle phase with upregulation of miR‑3160‑5p. Subsequent studies demonstrated that miR‑3160‑5p regulated the progression of the cell cycle in DU145 prostate cancer cells when the expression levels of phosphorylated cell division cycle (CDC)2, CDC25C and cyclin B1 were directly inhibited. Taken together, these findings revealed the mechanism underlying the role of miR‑3160‑5p in regulating the proliferation of DU145 cells and indicated that miR‑3160‑5p may serve as a promising novel therapeutic tool for prostate cancer.
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1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:25–29. 2015. View Article : Google Scholar

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Moore TH, King AJ, Evans M, Sharp D, Persad R and Huntley AL: Supportive care for men with prostate cancer: Why are the trials not working? A systematic review and recommendations for future trials. Cancer Med. 4:1240–1251. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Hudson SV, O'Malley DM and Miller SM: Achieving optimal delivery of follow-up care for prostate cancer survivors: Improving patient outcomes. Patient Relat Outcome Meas. 6:75–90. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Miyamoto H, Messing EM and Chang C: Androgen deprivation therapy for prostate cancer: Current status and future prospects. Prostate. 61:332–353. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Helsen C, Van den Broeck T, Voet A, Prekovic S, Van Poppel H, Joniau S and Claessens F: Androgen receptor antagonists for prostate cancer therapy. Endocr Relat Cancer. 21:T105–T118. 2014. View Article : Google Scholar : PubMed/NCBI

7 

Xu B, Huang Y, Niu X, Tao T, Jiang L, Tong N, Chen S, Liu N, Zhu W and Chen M: Hsa-miR-146a-5p modulates androgen-independent prostate cancer cells apoptosis by targeting ROCK1. Prostate. 75:1896–1903. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Khanmi K, Ignacimuthu S and Paulraj MG: MicroRNA in prostate cancer. Clin Chim Acta. 451:154–160. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Fang YX and Gao WQ: Roles of microRNAs during prostatic tumorigenesis and tumor progression. Oncogene. 33:135–147. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Hassan O, Ahmad A, Sethi S and Sarkar FH: Recent updates on the role of microRNAs in prostate cancer. J Hematol Oncol. 5:92012. View Article : Google Scholar : PubMed/NCBI

11 

Hsu TI, Hsu CH, Lee KH, Lin JT, Chen CS, Chang KC, Su CY, Hsiao M and Lu PJ: MicroRNA-18a is elevated in prostate cancer and promotes tumorigenesis through suppressing STK4 in vitro and in vivo. Oncogenesis. 3:e992014. View Article : Google Scholar : PubMed/NCBI

12 

Shi XB, Xue L, Yang J, Ma AH, Zhao J, Xu M, Tepper CG, Evans CP, Kung HJ and deVere White RW: An androgen-regulated miRNA suppresses Bak1 expression and induces androgen-independent growth of prostate cancer cells. Proc Natl Acad Sci USA. 104:19983–19988. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Wang L, Li B, Li L and Wang T: MicroRNA-497 suppresses proliferation and induces apoptosis in prostate cancer cells. Asian Pac J Cancer Prev. 14:3499–3502. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Nazarov PV, Reinsbach SE, Muller A, Nicot N, Philippidou D, Vallar L and Kreis S: Interplay of microRNAs, transcription factors and target genes: Linking dynamic expression changes to function. Nucleic Acids Res. 41:2817–2831. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Cellini F, Morganti AG, Genovesi D, Silvestris N and Valentini V: Role of microRNA in response to ionizing radiations: Evidences and potential impact on clinical practice for radiotherapy. Molecules. 19:5379–5401. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Dias DC, Dolios G, Wang R and Pan ZQ: CUL7: A DOC domain-containing cullin selectively binds Skp1. Fbx29 to form an SCF-like complex. Proc Natl Acad Sci USA. 99:16601–16606. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Huber C, Dias-Santagata D, Glaser A, O'Sullivan J, Brauner R, Wu K, Xu X, Pearce K, Wang R, Uzielli ML, et al: Identification of mutations in CUL7 in 3-M syndrome. Nat Genet. 37:1119–1124. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Xu X, Sarikas A, Dias-Santagata DC, Dolios G, Lafontant PJ, Tsai SC, Zhu W, Nakajima H, Nakajima HO, Field LJ, et al: The CUL7 E3 ubiquitin ligase targets insulin receptor substrate 1 for ubiquitin-dependent degradation. Mol Cell. 30:403–414. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Lin P, Fu J, Zhao B, Lin F, Zou H, Liu L, Zhu C, Wang H and Yu X: Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells. Mol Biol Rep. 38:1741–1747. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Shi D, Tan Z, Lu R, Yang W and Zhang Y: MicroRNA-218 inhibits the proliferation of human choriocarcinoma JEG-3 cell line by targeting Fbxw8. Biochem Biophys Res Commun. 450:1241–1246. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Hsieh IS, Chang KC, Tsai YT, Ke JY, Lu PJ, Lee KH, Yeh SD, Hong TM and Chen YL: MicroRNA-320 suppresses the stem cell-like characteristics of prostate cancer cells by downregulating the Wnt/beta-catenin signaling pathway. Carcinogenesis. 34:530–538. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Liu C, Kelnar K, Vlassov AV, Brown D, Wang J and Tang DG: Distinct microRNA expression profiles in prostate cancer stem/progenitor cells and tumor-suppressive functions of let-7. Cancer Res. 72:3393–3404. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Jin M, Zhang T, Liu C, Badeaux MA, Liu B, Liu R, Jeter C, Chen X, Vlassov AV and Tang DG: miRNA-128 suppresses prostate cancer by inhibiting BMI-1 to inhibit tumor-initiating cells. Cancer Res. 74:4183–4195. 2014. View Article : Google Scholar : PubMed/NCBI

27 

Kurisetty VV, Lakshmanaswamy R and Damodaran C: Pathogenic and therapeutic role of miRNAs in breast cancer. Front Biosci (Landmark Ed). 19:1–11. 2014. View Article : Google Scholar : PubMed/NCBI

28 

Okabe H, Lee SH, Phuchareon J, Albertson DG, McCormick F and Tetsu O: A critical role for FBXW8 and MAPK in cyclin D1 degradation and cancer cell proliferation. PLoS One. 1:e1282006. View Article : Google Scholar : PubMed/NCBI

29 

Wang H, Chen Y, Lin P, Li L, Zhou G, Liu G, Logsdon C, Jin J, Abbruzzese JL, Tan TH and Wang H: The CUL7/F-box and WD repeat domain containing 8 (CUL7/Fbxw8) ubiquitin ligase promotes degradation of hematopoietic progenitor kinase 1. J Biol Chem. 289:4009–4017. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Tsunematsu R, Nishiyama M, Kotoshiba S, Saiga T, Kamura T and Nakayama KI: Fbxw8 is essential for Cul1-Cul7 complex formation and for placental development. Mol Cell Biol. 26:6157–6169. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Molinari M: Cell cycle checkpoints and their inactivation in human cancer. Cell Prolif. 33:261–274. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Hartwell LH and Weinert TA: Checkpoints: Controls that ensure the order of cell cycle events. Science. 246:629–634. 1989. View Article : Google Scholar : PubMed/NCBI

33 

Chiang NJ, Lin CI, Liou JP, Kuo CC, Chang CY, Chen LT and Chang JY: A novel synthetic microtubule inhibitor, MPT0B214 exhibits antitumor activity in human tumor cells through mitochondria-dependent intrinsic pathway. PLoS One. 8:e589532013. View Article : Google Scholar : PubMed/NCBI

34 

King KL and Cidlowski JA: Cell cycle and apoptosis: Common pathways to life and death. J Cell Biochem. 58:175–180. 1995. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Lin P, Zhu L, Sun W, Yang Z, Sun H, Li D, Cui R, Zheng X and Yu X: Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8. Oncol Lett 15: 9436-9442, 2018.
APA
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D. ... Yu, X. (2018). Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8. Oncology Letters, 15, 9436-9442. https://doi.org/10.3892/ol.2018.8505
MLA
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D., Cui, R., Zheng, X., Yu, X."Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8". Oncology Letters 15.6 (2018): 9436-9442.
Chicago
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D., Cui, R., Zheng, X., Yu, X."Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8". Oncology Letters 15, no. 6 (2018): 9436-9442. https://doi.org/10.3892/ol.2018.8505
Copy and paste a formatted citation
x
Spandidos Publications style
Lin P, Zhu L, Sun W, Yang Z, Sun H, Li D, Cui R, Zheng X and Yu X: Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8. Oncol Lett 15: 9436-9442, 2018.
APA
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D. ... Yu, X. (2018). Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8. Oncology Letters, 15, 9436-9442. https://doi.org/10.3892/ol.2018.8505
MLA
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D., Cui, R., Zheng, X., Yu, X."Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8". Oncology Letters 15.6 (2018): 9436-9442.
Chicago
Lin, P., Zhu, L., Sun, W., Yang, Z., Sun, H., Li, D., Cui, R., Zheng, X., Yu, X."Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8". Oncology Letters 15, no. 6 (2018): 9436-9442. https://doi.org/10.3892/ol.2018.8505
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