Association between 5‑lipoxygenase expression, and malignant behaviors and poor prognosis in esophageal squamous cell carcinoma

Bai,Chun‑Ying; Zhang,Jun‑Yi; Shi,Tie‑Wei; Bai,Yu‑Qin; Wu,Bing‑Li; Du,Ze‑Peng; Wu,Zhi‑Yong; Xu,Xiu‑E; Wang,Shao‑Hong; Wu,Jian‑Yi; Te,Rui‑Yun; Zhang,Jing‑Yi; Xu,Li‑Yan; Li,En‑Min

doi:10.3892/ol.2018.8527

Association between 5‑lipoxygenase expression, and malignant behaviors and poor prognosis in esophageal squamous cell carcinoma

Authors:
- Chun‑Ying Bai
- Jun‑Yi Zhang
- Tie‑Wei Shi
- Yu‑Qin Bai
- Bing‑Li Wu
- Ze‑Peng Du
- Zhi‑Yong Wu
- Xiu‑E Xu
- Shao‑Hong Wang
- Jian‑Yi Wu
- Rui‑Yun Te
- Jing‑Yi Zhang
- Li‑Yan Xu
- En‑Min Li
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Affiliations: The Key Laboratory of Molecular Biology for High Cancer Incidence Coastal Chaoshan Area, Shantou University, Medical College, Shantou, Guangdong 515041, P.R. China, Research Center of Molecular Medicine, Medical College of Chifeng University, Chifeng, Inner Mongolia 024000, P.R. China, Department of Pathology, Medical College of Chifeng University, Chifeng, Inner Mongolia 024000, P.R. China, Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat‑sen University, Shantou, Guangdong 515041, P.R. China
Published online on: April 18, 2018 https://doi.org/10.3892/ol.2018.8527
Pages: 9353-9360

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Abstract

5‑lipoxygenase (5‑LO) catalyzes the first step of arachidonic acid metabolism to inflammatory mediator leukotrienes. The present study assessed 5‑LO expression in esophageal squamous cell carcinoma (ESCC) tissue specimens for associations with clinicopathological and survival data from patients, then explored 5‑LO activity in ESCC cells in vitro. 5‑LO expression was detected in tissue microarrays containing 297 ESCC samples using immunohistochemistry. Kaplan‑Meier curves were used to analyze the survival significance of 5‑LO expression and relative risk was evaluated using the multivariate Cox proportional hazards model. Cultured tumor cells were subjected to gene transfection, western blotting, and cell migration and proliferation assays. 5‑LO protein was primarily expressed in normal cell cytoplasm and/or membrane, and never in the whole cytoplasm, whereas 5‑LO was expressed diffusely in ESCC tissues with nearly homogeneous whole‑cytoplasm staining. 5‑LO expression was significantly associated with tumor regional lymph node metastasis (P=0.013) and pTNM stage (P=0.004). 5‑LO expression was associated with poor overall survival (P=0.029). Multivariate analysis demonstrated that 5‑LO overexpression was an independent prognostic factor for ESCC patients (P=0.041). Furthermore, the inhibition of 5‑LO expression reduced ESCC cell viability and migration in vitro. These data provide further evidence that the upregulation of 5‑LO expression is associated with advanced stages of disease and poor ESCC prognosis, and that 5‑LO expression may independently predict overall survival in patients with ESCC. The inhibition of 5‑LO expression reduced ESCC malignant behavior in vitro.

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