Effect of CXCR4 silencing with shRNA on MAPK signaling in ovarian cancer

  • Authors:
    • Xiuli Wang
    • Hongying Wang
    • Xia Wei
    • Aixia Wang
    • Lina Wen
    • Li Wang
    • Qun Huang
  • View Affiliations

  • Published online on: April 20, 2018     https://doi.org/10.3892/ol.2018.8550
  • Pages: 10026-10030
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Abstract

Our previous study demonstrated that short hairpin RNA (shRNA) targeting of C‑X‑C chemokine receptor type 4 (CXCR4) significantly inhibited cell proliferation, metastasis and invasion. On the basis of these results, the aim of the present study was to determine the effects of shRNA‑CXCR4 silencing on mitogen‑activated protein kinase (MAPK) signaling in human SW626 ovarian cancer cells. Following silencing the CXCR4 gene with shRNA, the mRNA expression of apoptosis signal‑regulating kinase 1 (ASK1) was determined using the reverse transcription‑quantitative polymerase chain reaction, whereas the protein expression of extracellular‑signal‑regulated kinase (ERK)1/2 and phosphorylated (p)‑c‑Jun were determined using immunocytochemistry and western blotting. SW626 cells transfected with shRNA‑CXCR4 exhibited significantly increased ASK1 mRNA expression (P<0.05), significantly increased p‑c‑Jun protein expression (P<0.05), and significantly decreased ERK1/2 protein expression (P<0.05). Silencing the CXCR4 gene with shRNA significantly inhibited cell proliferation, promoted cell apoptosis and may be mediated by the MAPK signaling pathway.
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June-2018
Volume 15 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Wang X, Wang H, Wei X, Wang A, Wen L, Wang L and Huang Q: Effect of CXCR4 silencing with shRNA on MAPK signaling in ovarian cancer. Oncol Lett 15: 10026-10030, 2018
APA
Wang, X., Wang, H., Wei, X., Wang, A., Wen, L., Wang, L., & Huang, Q. (2018). Effect of CXCR4 silencing with shRNA on MAPK signaling in ovarian cancer. Oncology Letters, 15, 10026-10030. https://doi.org/10.3892/ol.2018.8550
MLA
Wang, X., Wang, H., Wei, X., Wang, A., Wen, L., Wang, L., Huang, Q."Effect of CXCR4 silencing with shRNA on MAPK signaling in ovarian cancer". Oncology Letters 15.6 (2018): 10026-10030.
Chicago
Wang, X., Wang, H., Wei, X., Wang, A., Wen, L., Wang, L., Huang, Q."Effect of CXCR4 silencing with shRNA on MAPK signaling in ovarian cancer". Oncology Letters 15, no. 6 (2018): 10026-10030. https://doi.org/10.3892/ol.2018.8550