Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2018 Volume 15 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

  • Authors:
    • Weihua Ye
    • Yong Li
    • Liqiao Fan
    • Qun Zhao
    • Hufang Yuan
    • Bibo Tan
    • Zhidong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, The Third Department of Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 9836-9844
    |
    Published online on: April 25, 2018
       https://doi.org/10.3892/ol.2018.8576
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Annexin A7 is a member of the Annexin A family, which participates in various biological processes. Accumulating evidence has demonstrated that Annexin A7 serves an important role in tumorigenesis and is dysregulated in multiple types of cancer. However, the role of Annexin A7 in the tumorigenesis of gastric cancer remains to be determined. The present study revealed that Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis. There was a significant difference in Annexin A7 mRNA and protein expression in gastric cancer samples with distinct differentiation grades, with the lowest expression being observed in the highly differentiated cases. A terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling assay demonstrated that the apoptosis indices of highly, moderately and poorly differentiated gastric cancers were 18.12±2.40, 9.73±1.73 and 4.13±0.83%, respectively, with statistical significance (P<0.05). Flow cytometric analysis demonstrated that the apoptosis rates of gastric cancer MKN74, SGC7901 and BGC823 cells were 10.07±1.21, 7.11±1.04 and 4.25±1.02%, respectively, with statistical significance (P<0.05). Spearman's rank correlation analysis revealed that the Annexin A7 mRNA and protein levels were negatively correlated with the differentiation grade of the gastric cancer tissues, while the apoptosis index was positively correlated with the differentiation grade of the gastric cancer tissues. Furthermore, the apoptosis index was negatively correlated with Annexin A7 mRNA and protein expression. Similar associations were observed among Annexin A7 expression, differentiation grades and apoptosis in gastric cancer cell lines. The results of the present study demonstrated that Annexin A7 expression is downregulated, while apoptosis is upregulated, with the progression of gastric adenocarcinoma. These observations suggested that Annexin A7 may inhibit apoptosis during tumorigenesis and that it is a potential biomarker for the diagnosis, prognosis and treatment of gastric adenocarcinoma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Monastyrskaya K, Babiychuk EB and Draeger A: The Annexins: Spatial and temporal coordination of signaling events during cellular stress. Cell Mol Life Sci. 66:2623–2642. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Gerke V and Moss SE: Annexins: From structure to function. Physiol Rev. 82:331–371. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Gerke V, Creutz CE and Moss SE: Annexins: Linking Ca2+ signalling to membrane dynamics. Nat Rev Mol Cell Biol. 6:449–461. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Wang KL, Wu TT, Resetkova E, Wang H, Correa AM, Hofstetter WL, Swisher SG, Ajani JA, Rashid A, Hamilton SR and Albarracin CT: Expression of Annexin A1 in esophageal and esophagogastric junction adenocarcinomas: Association with poor outcome. Clin Cancer Res. 12:4598–4604. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Yan GR, Ding W, Xu SH, Xu Z, Xiao CL, Yin XF and He QY: Characterization of phosphoproteins in gastric cancer secretome. OMICS. 15:83–90. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Lauritzen SP, Boye TL and Nylandsted J: Annexins are instrumental for efficient plasma membrane repair in cancer cells. Semin Cell Dev Biol. 45:32–38. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Han Y, Ye J, Dong Y, Xu Z and Du Q: Expression and significance of Annexin A2 in patients with gastric adenocarcinoma and the association with E-cadherin. Exp Ther Med. 10:549–554. 2015. View Article : Google Scholar : PubMed/NCBI

9 

Creutz CE, Pazoles CJ and Pollard HB: Identification and purification of an adrenal medullary protein (synexin) that causes calcium-dependent aggregation of isolated chromaffin granules. J Biol Chem. 253:2858–2866. 1978.PubMed/NCBI

10 

Selbert S, Fischer P, Pongratz D, Stewart M and Noegel AA: Expression and localization of Annexin VII (synexin) in muscle cells. J Cell Sci. 108:85–95. 1995.PubMed/NCBI

11 

Clemen CS, Hofmann A, Zamparelli C and Noegel AA: Expression and localisation of Annexin VII (synexin) isoforms in differentiating myoblasts. J Muscle Res Cell Motil. 20:669–679. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Fatimathas L and Moss SE: Annexins as disease modifiers. Histol Histopathol. 25:527–532. 2010.PubMed/NCBI

13 

Huang Y, Du Y, Zhang X, Bai L, Mibrahim M, Zhang J, Wei Y, Li C, Fan S, Wang H, et al: Down-regulated expression of Annexin A7 induces apoptosis in mouse hepatocarcinoma cell line by the intrinsic mitochondrial pathway. Biomed Pharmacother. 70:146–150. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Song MY, Tang JW, Sun MZ, Liu SQ and Wang B: Localization and expression of CLIC1 in hepatocarcinoma ascites cell lines with high or low potentials of lymphatic spread. Zhonghua Bing Li Xue Za Zhi. 39:463–466. 2010.(In Chinese). PubMed/NCBI

15 

Srivastava M, Montagna C, Leighton X, Glasman M, Naga S, Eidelman O, Ried T and Pollard HB: Haploinsufficiency of Anx7 tumor suppressor gene and consequent genomic instability promotes tumorigenesis in the Anx7(+/-) mouse. Proc Natl Acad Sci USA. 100:14287–14292. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Srivastava M, Torosyan Y, Raffeld M, Eidelman O, Pollard HB and Bubendorf L: ANXA7 expression represents hormone-relevant tumor suppression in different cancers. Int J Cancer. 121:2628–2636. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Yadav AK, Renfrow JJ, Scholtens DM, Xie H, Duran GE, Bredel C, Vogel H, Chandler JP, Chakravarti A, Robe PA, et al: Monosomy of chromosome 10 associated with dysregulation of epidermal growth factor signaling in glioblastomas. JAMA. 302:276–289. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Hung KS and Howng SL: Prognostic significance of Annexin VII expression in glioblastomas multiforme in humans. J Neurosurg. 99:886–892. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Kataoka TR, Ito A, Asada H, Watabe K, Nishiyama K, Nakamoto K, Itami S, Yoshikawa K, Ito M, Nojima H and Kitamura Y: Annexin VII as a novel marker for invasive phenotype of malignant melanoma. Jpn J Cancer Res. 91:75–83. 2000. View Article : Google Scholar : PubMed/NCBI

20 

Torosyan Y, Dobi A, Glasman M, Mezhevaya K, Naga S, Huang W, Paweletz C, Leighton X, Pollard HB and Srivastava M: Role of multi-hnRNP nuclear complex in regulation of tumor suppressor ANXA7 in prostate cancer cells. Oncogene. 29:2457–2466. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Hsu PI, Huang MS, Chen HC, Hsu PN, Lai TC, Wang JL, Lo GH, Lai KH, Tseng CJ and Hsiao M: The significance of ANXA7 expression and its correlation with poor cellular differentiation and enhanced metastatic potential of gastric cancer. J Surg Oncol. 97:609–614. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Yang M and Liang Q: Study the relationship between the expression of Annexin A7 and CT of nasopharyngeal carcinoma. J Chin Clin Med Imaging. 22:6–9. 2011.

23 

Alfonso P, Canamero M, Fernández-Carbonié F, Núñez A and Casal JI: Proteome analysis of membrane fractions in colorectal carcinomas by using 2D-DIGE saturation labeling. J Proteome Res. 7:4247–4255. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Chen X, Gao FL, Chang YZ and Li X: Expression of Annexin A7 in human uterine cervical squamous carcinomas and normal tissues. Acta Anatomica Sinica. 41:603–605. 2010.

25 

Leighton X, Srikantan V, Pollard HB, Sukumar S and Srivastava M: Significant allelic loss of ANX7region (10q21) in hormone receptor negative breast carcinomas. Cancer Lett. 210:239–244. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Yuan HF, Li Y, Zhao Q, Fan LQ, Tan BB and Ye WH: Expression of Annexin A7 and its clinical significance in differentiation and metastasis of gastric carcinoma. Int J Clin Exp Pathol. 7:6567–6574. 2014.PubMed/NCBI

27 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

28 

Karube A, Shidara Y, Hayasaka K, Maki M and Tanaka T: Suppression of calphobindin I (CPB I) production in carcinoma of uterine cervix and endometrium. Gynecol Oncol. 58:295–300. 1995. View Article : Google Scholar : PubMed/NCBI

29 

Kanat O, O'Neil B and Shahda S: Targeted therapy for advanced gastric cancer: A review of current status and future prospects. World J Gastrointest Oncol. 7:401–410. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Xi JM and Zhao Q: Expression of Annexin A7 in gastric cancer tissues and their effects on the differentiation and metastasis of gastric cancer. J Exp Clin Med. 9:726–727. 2010.

31 

Gong X, Tang J and Geng X: Expression and significance of Annexin 7 in gastric cancer and lymphatic metastasis. Inter J Pathol Clin Med. 29:369–373. 2009.

32 

Giansanti V, Torriglia A and Scovassi AI: Conversation between apoptosis and autophagy: ‘Is it your turn or mine?’. Apoptosis. 16:321–333. 2011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ye W, Li Y, Fan L, Zhao Q, Yuan H, Tan B and Zhang Z: Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate. Oncol Lett 15: 9836-9844, 2018.
APA
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., & Zhang, Z. (2018). Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate. Oncology Letters, 15, 9836-9844. https://doi.org/10.3892/ol.2018.8576
MLA
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., Zhang, Z."Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate". Oncology Letters 15.6 (2018): 9836-9844.
Chicago
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., Zhang, Z."Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate". Oncology Letters 15, no. 6 (2018): 9836-9844. https://doi.org/10.3892/ol.2018.8576
Copy and paste a formatted citation
x
Spandidos Publications style
Ye W, Li Y, Fan L, Zhao Q, Yuan H, Tan B and Zhang Z: Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate. Oncol Lett 15: 9836-9844, 2018.
APA
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., & Zhang, Z. (2018). Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate. Oncology Letters, 15, 9836-9844. https://doi.org/10.3892/ol.2018.8576
MLA
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., Zhang, Z."Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate". Oncology Letters 15.6 (2018): 9836-9844.
Chicago
Ye, W., Li, Y., Fan, L., Zhao, Q., Yuan, H., Tan, B., Zhang, Z."Annexin A7 expression is downregulated in late‑stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate". Oncology Letters 15, no. 6 (2018): 9836-9844. https://doi.org/10.3892/ol.2018.8576
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team