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Article

Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis

  • Authors:
    • Wei‑Xin Cai
    • Ru Qing Yu
    • Li Ma
    • Hong Zhang Huang
    • Li Wu Zheng
    • Roger Zwahlen
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat‑Sen University, Guangzhou, Guangdong 510055, P.R. China, Department of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, SAR, P.R. China, Department of Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, SAR, P.R. China, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, Hong Kong, SAR, P.R. China
  • Pages: 9959-9964
    |
    Published online on: April 26, 2018
       https://doi.org/10.3892/ol.2018.8591
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Abstract

Distant metastasis represents the outcome with the worst prognosis for various types of malignant tumors, but little is known regarding the impact of interacting epithelial and mesenchymal phenotypic cancer cells within its etiopathogenesis. In a novel animal model, 48 male athymic Balb/c nude mice underwent subcutaneous and intravenous injection of human tongue cancer cell lines of green fluorescent mesenchymal and red fluorescent epithelial phenotypes, in order to visualize and monitor eventual phenotypic interaction in lung metastasis as well as experimental metastasis in in vivo, ex vivo and histopathological analyses. While the epithelial, but not the mesenchymal, phenotypic human tongue cancer cell line led to direct metastasis in the lungs when injected intravenously, neither of them, even when injected in combination, were able to establish distant metastasis. The results of the present study provide evidence regarding the role of epithelial phenotypic cancer cells in the release of experimental metastasis following tail vein injection in male athymic Balb/c nude mice, in addition to proving fluorescent human tongue cancer cells may be reliably detected under a fluorescence microscope even 8 weeks after the two injection types.
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Copy and paste a formatted citation
Spandidos Publications style
Cai WX, Yu RQ, Ma L, Huang HZ, Zheng LW and Zwahlen R: Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis. Oncol Lett 15: 9959-9964, 2018.
APA
Cai, W., Yu, R.Q., Ma, L., Huang, H.Z., Zheng, L.W., & Zwahlen, R. (2018). Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis. Oncology Letters, 15, 9959-9964. https://doi.org/10.3892/ol.2018.8591
MLA
Cai, W., Yu, R. Q., Ma, L., Huang, H. Z., Zheng, L. W., Zwahlen, R."Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis". Oncology Letters 15.6 (2018): 9959-9964.
Chicago
Cai, W., Yu, R. Q., Ma, L., Huang, H. Z., Zheng, L. W., Zwahlen, R."Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis". Oncology Letters 15, no. 6 (2018): 9959-9964. https://doi.org/10.3892/ol.2018.8591
Copy and paste a formatted citation
x
Spandidos Publications style
Cai WX, Yu RQ, Ma L, Huang HZ, Zheng LW and Zwahlen R: Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis. Oncol Lett 15: 9959-9964, 2018.
APA
Cai, W., Yu, R.Q., Ma, L., Huang, H.Z., Zheng, L.W., & Zwahlen, R. (2018). Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis. Oncology Letters, 15, 9959-9964. https://doi.org/10.3892/ol.2018.8591
MLA
Cai, W., Yu, R. Q., Ma, L., Huang, H. Z., Zheng, L. W., Zwahlen, R."Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis". Oncology Letters 15.6 (2018): 9959-9964.
Chicago
Cai, W., Yu, R. Q., Ma, L., Huang, H. Z., Zheng, L. W., Zwahlen, R."Differences between epithelial and mesenchymal human tongue cancer cell lines in experimental metastasis". Oncology Letters 15, no. 6 (2018): 9959-9964. https://doi.org/10.3892/ol.2018.8591
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