Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
August-2018 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2018 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways

  • Authors:
    • Xiaofeng Han
    • Jianwei Zheng
    • Yunlei Wang
    • Zhigang Gao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Beijing Chao‑Yang Hospital, Capital Medical University, Beijing 100043, P.R. China
  • Pages: 2638-2644
    |
    Published online on: June 5, 2018
       https://doi.org/10.3892/ol.2018.8905
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In the present study, the effects of microRNA‑29a (miRNA‑29a) on colon cancer cell viability and the molecular mechanisms underlying the effects were investigated. The expression of miRNA‑29a in colon cancer serum samples was notably downregulated, compared with in the normal group. First, miRNA‑29a mimic was used to increase the expression of miRNA‑29a in HCT‑116 cells. Furthermore, upregulation of miRNA‑29a suppressed cell viability, increased lactate dehydrogenase levels and apoptosis, and promoted caspase‑3/9 activities and B‑cell lymphoma 2‑associated X protein and phosphatase and tensin homolog (PTEN) protein expression in colon cancer cells. Furthermore, upregulation of miRNA‑29a decreased phosphoinositide 3‑kinase, phosphorylated (p)‑protein kinase B (Akt) and p‑glycogen synthase kinase 3β (GSK3β) protein expression and suppressed the Wnt/β‑catenin signaling pathway in colon cancer cells. The results of the present study verified that the protective effects of miRNA‑29a suppress the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways in colon cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Zhang HX, Wang ZT, Lu XX, Wang YG, Zhong J and Liu J: NLRP3 gene is associated with ulcerative colitis (UC), but not Crohn's disease (CD), in Chinese Han population. Inflamm Res. 63:979–985. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Zaki MH, Lamkanfi M and Kanneganti TD: The Nlrp3 inflammasome: contributions to intestinal homeostasis. Trends Immunol. 32:171–179. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Bank S, Andersen PS, Burisch J, Pedersen N, Roug S, Galsgaard J, Turino SY, Brodersen JB, Rashid S, Rasmussen BK, et al: Genetically determined high activity of IL-12 and IL-18 in ulcerative colitis and TLR5 in Crohns disease were associated with non-response to anti-TNF therapy. Pharmacogenomics J. 18:87–97. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Bauer C, Duewell P, Mayer C, Lehr HA, Fitzgerald KA, Dauer M, Tschopp J, Endres S, Latz E and Schnurr M: Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome. Gut. 59:1192–1199. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Engler DB, Leonardi I, Hartung ML, Kyburz A, Spath S, Becher B, Rogler G and Müller A: Helicobacter pylori-specific protection against inflammatory bowel disease requires the NLRP3 inflammasome and IL-18. Inflamm Bowel Dis. 21:854–861. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Gong Z, Zhou J, Zhao S, Tian C, Wang P, Xu C, Chen Y, Cai W and Wu J: Chenodeoxycholic acid activates NLRP3 inflammasome and contributes to cholestatic liver fibrosis. Oncotarget. 7:83951–83963. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Ten Hove T, Corbaz A, Amitai H, Aloni S, Belzer I, Graber P, Drillenburg P, van Deventer SJ, Chvatchko Y and Te Velde AA: Blockade of endogenous IL-18 ameliorates TNBS-induced colitis by decreasing local TNF-alpha production in mice. Gastroenterology. 121:1372–1379. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Inoue A, Mizushima T, Wu X, Okuzaki D, Kambara N, Ishikawa S, Wang J, Qian Y, Hirose H, Yokoyama Y, et al: A miR-29b byproduct sequence exhibits potent tumor-suppressive activities via inhibition of NF-kappaB signaling in KRAS-mutant colon cancer cells. Mol Cancer Ther. 17:977–987. 2018. View Article : Google Scholar : PubMed/NCBI

9 

Wang N, Zhang Y and Liang H: microRNA-598 inhibits cell proliferation and invasion of glioblastoma by directly targeting metastasis associated in colon cancer-1. Oncol Res. 2018, Feb 14. Doi: 10.3727/096504018X15185735627746. View Article : Google Scholar

10 

Bruusgaard A and Andersen RB: Chenodeoxycholic-acid treatments of rheumatoid arthritis. Lancet. 1:7001976. View Article : Google Scholar : PubMed/NCBI

11 

Li Q, Hu X, Xuan Y, Ying J, Fei Y, Rong J, Zhang Y, Zhang J, Liu C and Liu Z: Kaempferol protects ethanol-induced gastric ulcers in mice via pro-inflammatory cytokines and NO. Acta Biochim Biophys Sin (Shanghai). 50:246–253. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Park GB, Chung YH, Gong JH, Jin DH and Kim D: GSK-3β-mediated fatty acid synthesis enhances epithelial to mesenchymal transition of TLR4-activated colorectal cancer cells through regulation of TAp63. Int J Oncol. 49:2163–2172. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Martínez-Martínez E, Martín-Ruiz A, Martín P, Calvo V, Provencio M and García JM: CB2 cannabinoid receptor activation promotes colon cancer progression via AKT/GSK3beta signaling pathway. Oncotarget. 7:68781–68791. 2016. View Article : Google Scholar : PubMed/NCBI

14 

Jendželovský R, Koval J, Mikeš J, Papčová Z, Plšíková J and Fedoročko P: Inhibition of GSK-3beta reverses the pro-apoptotic effect of proadifen (SKF-525A) in HT-29 colon adenocarcinoma cells. Toxicol In Vitro. 26:775–782. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Jamwal G, Singh G, Dar MS, Singh P, Bano N, Syed SH, Sandhu P, Akhter Y, Monga SP and Dar MJ: Identification of a unique loss-of-function mutation in IGF1R and a crosstalk between IGF1R and Wnt/β-catenin signaling pathways. Biochim Biophys Acta. 1865:920–931. 2018. View Article : Google Scholar : PubMed/NCBI

16 

Wang W, Li Y, Chen Y, Chen H, Zhu P, Xu M, Wang H, Wu M, Yang Z, Hoffman RM and Gu Y: Ethanolic extract of traditional chinese medicine (TCM) gamboge inhibits colon cancer via the Wnt/beta-catenin signaling pathway in an orthotopic mouse model. Anticancer Res. 38:1917–1925. 2018.PubMed/NCBI

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Lazaridis LD, Pistiki A, Giamarellos-Bourboulis EJ, Georgitsi M, Damoraki G, Polymeros D, Dimitriadis GD and Triantafyllou K: Activation of NLRP3 inflammasome in inflammatory bowel disease: Differences between crohn's disease and ulcerative colitis. Dig Dis Sci. 62:2348–2356. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Itani S, Watanabe T, Nadatani Y, Sugimura N, Shimada S, Takeda S, Otani K, Hosomi S, Nagami Y, Tanaka F, et al: NLRP3 inflammasome has a protective effect against oxazolone-induced colitis: A possible role in ulcerative colitis. Sci Rep. 6:390752016. View Article : Google Scholar : PubMed/NCBI

20 

Choi EM: Kaempferol protects MC3T3-E1 cells through antioxidant effect and regulation of mitochondrial function. Food Chem Toxicol. 49:1800–1805. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Chen X, Yan L, Guo Z, Chen Y, Li M, Huang C, Chen Z and Meng X: Chenodeoxycholic acid attenuates high-fat diet-induced obesity and hyperglycemia via the G protein-coupled bile acid receptor 1 and proliferator-activated receptor γ pathway. Exp Ther Med. 14:5305–5312. 2017.PubMed/NCBI

22 

Hirano Y, Hirano F, Fujii H and Makino I: Fibrates suppress chenodeoxycholic acid-induced RANTES expression through inhibition of NF-kappaB activation. Eur J Pharmacol. 448:19–26. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Chen X, Qian J, Wang L, Li J, Zhao Y, Han J, Khan Z, Chen X, Wang J and Liang G: Kaempferol attenuates hyperglycemia-induced cardiac injuries by inhibiting inflammatory responses and oxidative stress. Endocrine. 60:83–94. 2018. View Article : Google Scholar : PubMed/NCBI

24 

Kim SH, Park JG, Lee J, Yang WS, Park GW, Kim HG, Yi YS, Baek KS, Sung NY, Hossen MJ, et al: The dietary flavonoid Kaempferol mediates anti-inflammatory responses via the Src, Syk, IRAK1, and IRAK4 molecular targets. Mediators Inflamm. 2015:9041422015. View Article : Google Scholar : PubMed/NCBI

25 

Li R, Liu J, Li Q, Chen G and Yu X: miR-29a suppresses growth and metastasis in papillary thyroid carcinoma by targeting AKT3. Tumour Biol. 37:3987–3996. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Zhuang Z, Ye G and Huang B: Kaempferol Alleviates the Interleukin-1β-induced inflammation in rat osteoarthritis chondrocytes via suppression of NF-kappaB. Med Sci Monit. 23:3925–3931. 2017. View Article : Google Scholar : PubMed/NCBI

27 

Cheng X, Yang YL, Yang H, Wang YH and Du GH: Kaempferol alleviates LPS-induced neuroinflammation and BBB dysfunction in mice via inhibiting HMGB1 release and down-regulating TLR4/MyD88 pathway. Int Immunopharmacol. 56:29–35. 2018. View Article : Google Scholar : PubMed/NCBI

28 

Shen H, Li L, Yang S, Wang D, Zhong S, Zhao J and Tang J: MicroRNA-29a contributes to drug-resistance of breast cancer cells to adriamycin through PTEN/AKT/GSK3β signaling pathway. Gene. 593:84–90. 2016. View Article : Google Scholar : PubMed/NCBI

29 

Basu A, Das AS, Sharma M, Pathak MP, Chattopadhyay P, Biswas K and Mukhopadhyay R: STAT3 and NF-kappaB are common targets for kaempferol-mediated attenuation of COX-2 expression in IL-6-induced macrophages and carrageenan-induced mouse paw edema. Biochem Biophys Rep. 12:54–61. 2017.PubMed/NCBI

30 

Merhi A, de Mees C, Abdo R, Victoria Alberola J and Marini AM: Wnt/β-catenin signaling regulates the expression of the ammonium permease gene RHBG in human cancer cells. PLoS One. 10:e01286832015. View Article : Google Scholar : PubMed/NCBI

31 

Mervai Z, Sólyomváry A, Tóth G, Noszál B, Molnár-Perl I, Baghy K, Kovalszky I and Boldizsár I: Endogenous enzyme-hydrolyzed fruit of Cirsium brachycephalum: Optimal source of the antiproliferative lignan trachelogenin regulating the Wnt/beta-catenin signaling pathway in the SW480 colon adenocarcinoma cell line. Fitoterapia. 100:19–26. 2015. View Article : Google Scholar : PubMed/NCBI

32 

Voloshanenko O, Erdmann G, Dubash TD, Augustin I, Metzig M, Moffa G, Hundsrucker C, Kerr G, Sandmann T, Anchang B, et al: Wnt secretion is required to maintain high levels of Wnt activity in colon cancer cells. Nat Commun. 4:26102013. View Article : Google Scholar : PubMed/NCBI

33 

Nagano H, Tomimaru Y, Eguchi H, Hama N, Wada H, Kawamoto K, Kobayashi S, Mori M and Doki Y: MicroRNA-29a induces resistance to gemcitabine through the Wnt/β-catenin signaling pathway in pancreatic cancer cells. Int J Oncol. 43:1066–1072. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Han X, Zheng J, Wang Y and Gao Z: miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncol Lett 16: 2638-2644, 2018.
APA
Han, X., Zheng, J., Wang, Y., & Gao, Z. (2018). miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncology Letters, 16, 2638-2644. https://doi.org/10.3892/ol.2018.8905
MLA
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16.2 (2018): 2638-2644.
Chicago
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16, no. 2 (2018): 2638-2644. https://doi.org/10.3892/ol.2018.8905
Copy and paste a formatted citation
x
Spandidos Publications style
Han X, Zheng J, Wang Y and Gao Z: miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncol Lett 16: 2638-2644, 2018.
APA
Han, X., Zheng, J., Wang, Y., & Gao, Z. (2018). miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways. Oncology Letters, 16, 2638-2644. https://doi.org/10.3892/ol.2018.8905
MLA
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16.2 (2018): 2638-2644.
Chicago
Han, X., Zheng, J., Wang, Y., Gao, Z."miRNA‑29a inhibits colon cancer growth by regulation of the PTEN/Akt/GSK3β and Wnt/β‑catenin signaling pathways". Oncology Letters 16, no. 2 (2018): 2638-2644. https://doi.org/10.3892/ol.2018.8905
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team