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Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway

  • Authors:
    • Jinxia Peng
    • Rui Liu
    • Ling Peng
    • Hongtao Jia
  • View Affiliations / Copyright

    Affiliations: Department of Burn and Plastic Surgery, Shiyan People's Hospital, People's Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Oncology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China, Department of Health Management, People's Hospital of Shiyan Economic Development Zone, Shiyan, Hubei 442000, P.R. China, Department of Urological Surgery, Shiyan People's Hospital, People's Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
    Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2921-2928
    |
    Published online on: June 18, 2018
       https://doi.org/10.3892/ol.2018.8975
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Abstract

The present study was performed to determine the molecular mechanism of calcium gluconate (CG) in alleviating the toxic effect of hydrofluoric (HF) acid on human dermal fibroblasts (HDFs). HDF morphology was observed by optical microscopy and the vimentin immunofluorescence assay. Cell viability and apoptosis were evaluated by the Cell Counting Kit‑8 and Annexin V/propidium iodide assays, respectively. The levels of apoptosis‑associated factors, as well as Wnt2, Wnt3a and β‑catenin were detected by reverse transcription‑quantitative polymerase chain reaction and western blotting. Levels of matrix metalloproteinase (MMP)‑1 and basic fibroblast growth factor (bFGF) were detected by ELISA and western blotting. Carboxyterminal propeptide of type Ⅰ collagen (CICP) was detected by ELISA, while L‑Hydroxyproline (L‑HYP) was detected by colorimetry. First, the morphology of normal HDFs was observed. Cell viability was inhibited and apoptosis was increased in a dose‑ and time‑dependent manner following treatment with HF acid [0, 2, 4, 6, 8, 10 and 20% (v/v)] for 0, 2, 4, 6, 8, 10 and 20 min. The effects were blocked by CG at different doses (50, 100 and 200 µmol/l) and time points (6, 12 and 24 h), following treatment with 8% (v/v) HF acid for 6 min. The levels of Caspase‑3, B‑cell lymphoma (Bcl)‑2 associated X protein, Wnt2, Wnt3a and β‑catenin were decreased, whereas Bcl‑2 was increased by CG treatment dose‑dependently, when compared with HF control. CG promoted the expression of MMP‑1, bFGF and L‑HYP, and inhibited CICP, when compared with HF control. Based on the present results, CG alleviated the toxic effect of HF acid on HDFs by regulating the Wnt/β‑catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Peng J, Liu R, Peng L and Jia H: Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway. Oncol Lett 16: 2921-2928, 2018.
APA
Peng, J., Liu, R., Peng, L., & Jia, H. (2018). Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway. Oncology Letters, 16, 2921-2928. https://doi.org/10.3892/ol.2018.8975
MLA
Peng, J., Liu, R., Peng, L., Jia, H."Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway". Oncology Letters 16.3 (2018): 2921-2928.
Chicago
Peng, J., Liu, R., Peng, L., Jia, H."Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway". Oncology Letters 16, no. 3 (2018): 2921-2928. https://doi.org/10.3892/ol.2018.8975
Copy and paste a formatted citation
x
Spandidos Publications style
Peng J, Liu R, Peng L and Jia H: Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway. Oncol Lett 16: 2921-2928, 2018.
APA
Peng, J., Liu, R., Peng, L., & Jia, H. (2018). Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway. Oncology Letters, 16, 2921-2928. https://doi.org/10.3892/ol.2018.8975
MLA
Peng, J., Liu, R., Peng, L., Jia, H."Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway". Oncology Letters 16.3 (2018): 2921-2928.
Chicago
Peng, J., Liu, R., Peng, L., Jia, H."Calcium gluconate alleviates the toxic effect of hydrofluoric acid on human dermal fibroblasts through the Wnt/β‑catenin pathway". Oncology Letters 16, no. 3 (2018): 2921-2928. https://doi.org/10.3892/ol.2018.8975
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