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Article

Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner

  • Authors:
    • Russalina Stroeder
    • Barbara Walch‑Rückheim
    • Jil Fischbach
    • Ingolf Juhasz‑Böss
    • Christian Rübe
    • Erich‑Franz Solomayer
    • Sigrun Smola
  • View Affiliations / Copyright

    Affiliations: Department of Gynecology and Obstetrics, Saarland University, D‑66421 Homburg/Saar, Germany, Institute of Virology, Saarland University, D‑66421 Homburg/Saar, Germany, Department of Radiotherapy and Radiation Oncology, Saarland University, D‑66421 Homburg/Saar, Germany
  • Pages: 3351-3358
    |
    Published online on: June 18, 2018
       https://doi.org/10.3892/ol.2018.8987
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Abstract

Cervical cancer stage‑dependent therapies include surgery, chemotherapy, radiotherapy and chemoradiotherapy. Concurrent cisplatin‑based chemoradiotherapy (CCRT) is the standard therapy for locally advanced cervical carcinoma (FIGO>IIB), however therapy resistance in a subset of patients is still a major clinical challenge. The present study aimed to analyze the impact of Oncostatin M (OSM) stimulation on CCRT‑induced cell death. The present study used cells derived from cervical squamous cell carcinomas (SW756, 808, CaSki and 879) and adenocarcinoma (HeLa). The cervical carcinoma cells were HPV18‑positive (HeLa, SW756, 808) or HPV16‑positive (CaSki, 879). In addition to the established cell lines HeLa, SW756 and CaSki, the more recently generated cervical cancer cells 808 and 879 were also used. To analyze their radiosensitivity, cells were treated with increasing doses of irradiation (0‑8 Gy). To mimic chemotherapy, radiotherapy or CCRT in vitro, the cells were challenged with 0.975 µg/ml cisplatin, irradiated with 6 Gy or a combination. A total of 10 ng/ml OSM was applied for 2 h prior to the respective therapy. The responsiveness toward radiation alone varied among the cervical carcinoma cells. CaSki, 808 and 879 cells were resistant to irradiation up to 8 Gy. OSM pre‑treatment sensitized two out of five cell lines (HeLa and 879) to irradiation. Notably, all tested cells were sensitized by OSM for CCRT‑treatment, particularly in the less radiosensitive cells. Cell death enhancement was dependent on phosphorylated signal transducer and activator of transcription 3 (STAT3; Tyr705) signaling activation as demonstrated with a dominant‑negative version of STAT3 interfering with phosphorylation at Tyr705 (dnSTAT3‑Y705F). In conclusion, OSM pre‑treatment was able to override resistance to CCRT via the STAT3 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Stroeder R, Walch‑Rückheim B, Fischbach J, Juhasz‑Böss I, Rübe C, Solomayer EF and Smola S: Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner. Oncol Lett 16: 3351-3358, 2018.
APA
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., & Smola, S. (2018). Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner. Oncology Letters, 16, 3351-3358. https://doi.org/10.3892/ol.2018.8987
MLA
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., Smola, S."Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner". Oncology Letters 16.3 (2018): 3351-3358.
Chicago
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., Smola, S."Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner". Oncology Letters 16, no. 3 (2018): 3351-3358. https://doi.org/10.3892/ol.2018.8987
Copy and paste a formatted citation
x
Spandidos Publications style
Stroeder R, Walch‑Rückheim B, Fischbach J, Juhasz‑Böss I, Rübe C, Solomayer EF and Smola S: Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner. Oncol Lett 16: 3351-3358, 2018.
APA
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., & Smola, S. (2018). Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner. Oncology Letters, 16, 3351-3358. https://doi.org/10.3892/ol.2018.8987
MLA
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., Smola, S."Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner". Oncology Letters 16.3 (2018): 3351-3358.
Chicago
Stroeder, R., Walch‑Rückheim, B., Fischbach, J., Juhasz‑Böss, I., Rübe, C., Solomayer, E., Smola, S."Oncostatin M treatment increases the responsiveness toward cisplatin‑based chemoradiotherapy in cervical cancer cells in a STAT3‑dependent manner". Oncology Letters 16, no. 3 (2018): 3351-3358. https://doi.org/10.3892/ol.2018.8987
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