Open Access

AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells

  • Authors:
    • Weigang Xiu
    • Juan Ma
    • Ting Lei
    • Man Zhang
  • View Affiliations

  • Published online on: June 27, 2018     https://doi.org/10.3892/ol.2018.9028
  • Pages: 2851-2856
  • Copyright: © Xiu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Past studies have confirmed that tumors can impair the function of dendritic cells (DCs) and promote tumor evasion. AG490, a Janus kinase 2/signal transducer and activator of transcription 3 inhibitor, has been shown to induce maturation of DCs and inhibit the growth of tumor cells. In the present study, DCs were generated from healthy human peripheral blood mononuclear cells. On day 5 of culture, the DCs were co‑cultured with human bladder cancer pumc‑91 cells for 24 h, and then purified using magnetic beads. The maturation of the DCs was induced by lipopolysaccharide. Subsequent to co‑culture with pumc‑91 cells, the expression of human leukocyte antigen‑antigen D related (HLA‑DR), cluster of differentiation (CD)86 and CD80 was found to be reduced in the DCs, accompanied by increased production of interleukin (IL)‑10, but decreased production of IL‑12p70. Furthermore, the DCs co‑cultured with pumc‑91 inhibited the proliferation of allogeneic T cells. Finally, AG490 restored the expression of HLA‑DR, CD86 and CD80. These data identified that bladder cancer cells could inhibit the antigen‑presenting function of the DCs and induce anergy in T cells. AG490 may partly reverse this inhibitory effect of bladder cancer cells on DCs, activate immunogenicity and induce the antitumor immunity response of DCs.
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September-2018
Volume 16 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Xiu W, Ma J, Lei T and Zhang M: AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells. Oncol Lett 16: 2851-2856, 2018
APA
Xiu, W., Ma, J., Lei, T., & Zhang, M. (2018). AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells. Oncology Letters, 16, 2851-2856. https://doi.org/10.3892/ol.2018.9028
MLA
Xiu, W., Ma, J., Lei, T., Zhang, M."AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells". Oncology Letters 16.3 (2018): 2851-2856.
Chicago
Xiu, W., Ma, J., Lei, T., Zhang, M."AG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells". Oncology Letters 16, no. 3 (2018): 2851-2856. https://doi.org/10.3892/ol.2018.9028