Knockdown of lncRNA TP73‑AS1 inhibits gastric cancer cell proliferation and invasion via the WNT/β‑catenin signaling pathway

Wang,Yufeng; Xiao,Shuai; Wang,Bingyi; Li,Yang; Chen,Quanning

doi:10.3892/ol.2018.9040

Knockdown of lncRNA TP73‑AS1 inhibits gastric cancer cell proliferation and invasion via the WNT/β‑catenin signaling pathway

Authors:
- Yufeng Wang
- Shuai Xiao
- Bingyi Wang
- Yang Li
- Quanning Chen
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Affiliations: Department of General Surgery, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, P.R. China
Published online on: June 28, 2018 https://doi.org/10.3892/ol.2018.9040
Pages: 3248-3254

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Abstract

Long non‑coding RNAs (lncRNAs) function as tumor suppressors or oncogenes in tumor development and progression. The purpose of the present study was to investigate the clinical significance and functional role of lncRNA TP73‑AS1 in gastric cancer (GC). Reverse transcription‑quantitative polymerase chain reaction analysis demonstrated that TP73‑AS1 was significantly upregulated in GC tissues compared with adjacent normal tissues. The higher expression of TP73‑AS1 was closely associated with lymph node metastasis and tumor‑node‑metastasis stage in patients with GC. Those patients with GC showing a higher expression of TP73‑AS1 were predicted to have shorter disease‑free survival and overall survival rates. The knockdown of TP73‑AS1 was shown to markedly inhibit cell proliferation, cell colony formation and cell invasion. In addition, the downregulation of TP73‑AS1 suppressed the expression of transcription factor 4 and β‑catenin, which suggested that a decrease in TP73‑AS1 suppressed the WNT/β‑catenin signaling pathway in GC. Therefore, these results indicated that TP73‑AS1 may be a target for GC treatment.

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1	Jemal A, Bray F, Center MM, Ferlay J, Ward E and Forman D: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar : PubMed/NCBI
2	Ohtsu A: Chemotherapy for metastatic gastric cancer: Past, present, and future. J Gastroenterol. 43:256–264. 2008. View Article : Google Scholar : PubMed/NCBI
3	Wang KC and Chang HY: Molecular mechanisms of long noncoding RNAs. Mol Cell. 43:904–914. 2011. View Article : Google Scholar : PubMed/NCBI
4	Li Y and Wang X: Role of long noncoding RNAs in malignant disease (Review). Mol Med Rep. 13:1463–1469. 2016. View Article : Google Scholar : PubMed/NCBI
5	Zhou M, Zhang XY and Yu X: Overexpression of the long non-coding RNA SPRY4-IT1 promotes tumor cell proliferation and invasion by activating EZH2 in hepatocellular carcinoma. Biomed Pharmacother. 85:348–354. 2017. View Article : Google Scholar : PubMed/NCBI
6	Fan Y, Wang YF, Su HF, Fang N, Zou C, Li WF and Fei ZH: Decreased expression of the long noncoding RNA LINC00261 indicate poor prognosis in gastric cancer and suppress gastric cancer metastasis by affecting the epithelial-mesenchymal transition. J Hematol Oncol. 9:572016. View Article : Google Scholar : PubMed/NCBI
7	Deng QJ, Xie LQ and Li H: Overexpressed MALAT1 promotes invasion and metastasis of gastric cancer cells via increasing EGFL7 expression. Life Sci. 157:38–44. 2016. View Article : Google Scholar : PubMed/NCBI
8	Zhang E, He X, Yin D, Han L, Qiu M, Xu T, Xia R, Xu L, Yin R and De W: Increased expression of long noncoding RNA TUG1 predicts a poor prognosis of gastric cancer and regulates cell proliferation by epigenetically silencing of p57. Cell Death Dis. 7:e21092016. View Article : Google Scholar : PubMed/NCBI
9	Zang W, Wang T, Wang Y, Chen X, Du Y, Sun Q, Li M, Dong Z and Zhao G: Knockdown of long non-coding RNA TP73-AS1 inhibits cell proliferation and induces apoptosis in esophageal squamous cell carcinoma. Oncotarget. 7:19960–19974. 2016. View Article : Google Scholar : PubMed/NCBI
10	Zhang R, Jin H and Lou F: The long non-coding RNA TP73-AS1 interacted with miR-142 to modulate brain glioma growth through HMGB1/RAGE pathway. J Cell Biochem. 119:3007–3016. 2018. View Article : Google Scholar : PubMed/NCBI
11	Li S, Huang Y, Huang Y, Fu Y, Tang D, Kang R, Zhou R and Fan XG: The long non-coding RNA TP73-AS1 modulates HCC cell proliferation through miR-200a-dependent HMGB1/RAGE regulation. J Exp Clin Cancer Res. 36:512017. View Article : Google Scholar : PubMed/NCBI
12	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
13	Wu F, Li J, Guo N, Wang XH and Liao YQ: MiRNA-27a promotes the proliferation and invasion of human gastric cancer MGC803 cells by targeting SFRP1 via Wnt/β-catenin signaling pathway. Am J Cancer Res. 7:405–416. 2017.PubMed/NCBI
14	Jin C, Shi W, Wang F, Shen X, Qi J, Cong H, Yuan J, Shi L, Zhu B, Luo X, et al: Long non-coding RNA HULC as a novel serum biomarker for diagnosis and prognosis prediction of gastric cancer. Oncotarget. 7:51763–51772. 2016. View Article : Google Scholar : PubMed/NCBI
15	Ye H, Liu K and Qian K: Overexpression of long noncoding RNA HOTTIP promotes tumor invasion and predicts poor prognosis in gastric cancer. Onco Targets Ther. 9:2081–2088. 2016.PubMed/NCBI
16	Zhou X, Yin C, Dang Y, Ye F and Zhang G: Identification of the long non-coding RNA H19 in plasma as a novel biomarker for diagnosis of gastric cancer. Sci Rep. 5:115162015. View Article : Google Scholar : PubMed/NCBI
17	Wang JB, Liu FH, Chen JH, Ge HT, Mu LY, Bao HB and Lin ZG: Identifying survival-associated modules from the dysregulated triplet network in glioblastoma multiforme. J Cancer Res Clin Oncol. 143:661–671. 2017. View Article : Google Scholar : PubMed/NCBI
18	Clevers H and Nusse R: Wnt/β-catenin signaling and disease. Cell. 149:1192–1205. 2012. View Article : Google Scholar : PubMed/NCBI
19	Nuñez F, Bravo S, Cruzat F, Montecino M and De Ferrari GV: Wnt/β-catenin signaling enhances cyclooxygenase-2 (COX2) transcriptional activity in gastric cancer cells. PLoS One. 6:e185622011. View Article : Google Scholar : PubMed/NCBI
20	Zhou Y, Wang Y, Wen J, Zhao H, Dong X, Zhang Z, Wang S and Shen L: Aquaporin 3 promotes the stem-like properties of gastric cancer cells via Wnt/GSK-3β/β-catenin pathway. Oncotarget. 7:16529–16541. 2016.PubMed/NCBI