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Article

Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells

  • Authors:
    • Wei He
    • Tong Yang
    • Xin‑Hua Gong
    • Ru‑Zhai Qin
    • Xiao‑Dong Zhang
    • Wen‑Dan Liu
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 511447, P.R. China, Department of Pathology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 511447, P.R. China, Department of General Medicine, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 511447, P.R. China
  • Pages: 3976-3982
    |
    Published online on: July 4, 2018
       https://doi.org/10.3892/ol.2018.9076
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Abstract

An increasing volume of data indicates that disrupting the interaction between CXC motif chemokine receptor 4 (CXCR4) and its specific ligand, CXC motif chemokine 12 (CXCL12), may reduce tumor growth and metastasis. However, the translation from bench to bedside must be performed with extreme caution, as the CXCR4/CXCL12 axis is crucial for the normal development and maintenance of tissues and organs. In the present study, Cell Counting Kit‑8 and Transwell migration assays were used to detect in vitro proliferation and chemotaxis of CXCR4‑expressing A549 cells, a cell strain originating from human non‑small‑cell lung cancer (NSCLC), with or without the presence of AMD3100, a small‑molecule inhibitor specific to CXCR4 signaling. In a xenograft model established by injecting nude mice with A549 cells, tumor growth, CXCR4 expression and microvessel density (MVD) in the tumor mass were determined through tumor size measurements and immunohistochemical staining following intraperitoneal administration of AMD3100 or vehicle. The results demonstrated that CXCR4 blockade inhibited the proliferation of A549 cells and their migration towards CXCL12 in vitro. Tumor growth, CXCR4 expression and MVD were markedly reduced in nude mice treated with AMD3100 compared with mice treated with the vehicle. In conclusion, the present data demonstrated that CXCR4 targeting impaired NSCLC cell growth, angiogenesis and metastatic spread, indicating that it may represent a novel treatment strategy for NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
He W, Yang T, Gong XH, Qin RZ, Zhang XD and Liu WD: Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells. Oncol Lett 16: 3976-3982, 2018.
APA
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., & Liu, W. (2018). Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells. Oncology Letters, 16, 3976-3982. https://doi.org/10.3892/ol.2018.9076
MLA
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., Liu, W."Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells". Oncology Letters 16.3 (2018): 3976-3982.
Chicago
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., Liu, W."Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells". Oncology Letters 16, no. 3 (2018): 3976-3982. https://doi.org/10.3892/ol.2018.9076
Copy and paste a formatted citation
x
Spandidos Publications style
He W, Yang T, Gong XH, Qin RZ, Zhang XD and Liu WD: Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells. Oncol Lett 16: 3976-3982, 2018.
APA
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., & Liu, W. (2018). Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells. Oncology Letters, 16, 3976-3982. https://doi.org/10.3892/ol.2018.9076
MLA
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., Liu, W."Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells". Oncology Letters 16.3 (2018): 3976-3982.
Chicago
He, W., Yang, T., Gong, X., Qin, R., Zhang, X., Liu, W."Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells". Oncology Letters 16, no. 3 (2018): 3976-3982. https://doi.org/10.3892/ol.2018.9076
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