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Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line

  • Authors:
    • Yanzhen Zuo
    • Qian Xu
    • Yanjie Lu
    • Dayong Sun
    • Kang Wang
    • Yuntao Lei
    • Xiujun Liang
    • Yuhong Li
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacology, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Research Laboratory, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Department of Pathology, Chengde Medical University, Chengde, Hebei 067000, P.R. China, Department of Tumor Radiation and Chemotherapy Center, The Chengde Central Hospital, Chengde, Hebei 067000, P.R. China
    Copyright: © Zuo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4229-4234
    |
    Published online on: July 26, 2018
       https://doi.org/10.3892/ol.2018.9220
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Abstract

Choriocarcinoma is a malignant trophoblastic tumor. The development of novel drugs is required to reduce the toxicity of current multi‑agent chemotherapy and to successfully treat chemoresistant cases of the disease. The purpose of the present study was to investigate the effect of dihydromyricetin (DMY) on the human choriocarcinoma cell line, JAr, to identify a novel drug for the treatment of choriocarcinoma. An MTT assay was performed to determine the effects of DMY at different concentrations and for different exposure durations. Flow cytometry and TUNEL assays were performed to detect apoptosis, and western blotting was utilized to investigate the underlying mechanism. The results revealed that DMY significantly inhibited JAr cell viability in a time‑ and dose‑dependent manner. The flow cytometry and TUNEL assays demonstrated that DMY inhibited proliferation by inducing apoptosis. Further analysis by western blotting indicated that the protein expression level of BCL‑2 associated X, associated protein increased, while the protein expression levels of BCL‑2 and pro‑caspase‑3 decreased. These findings suggest that DMY induced apoptosis in human choriocarcinoma JAr cells, through a mitochondrially mediated apoptotic pathway.
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1 

Xu Q, Tan Y, Zhang K and Li Y: Crosstalk between p38 and Smad3 through TGF-β1 in JEG-3 choriocarcinoma cells. Int J Oncol. 43:1187–1193. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Tan Y, Xu Q, Li Y, Mao X and Zhang K: Crosstalk between the p38 and TGF-β signaling pathways through TβRI, TβRII and Smad3 expression in plancental choriocarcinoma JEG-3 cells. Oncol Lett. 8:1307–1311. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Yamamoto E, Ino K, Yamamoto T, Sumigama S, Nawa A, Nomura S and Kikkawa F: A pure nongestational choriocarcinoma of the ovary diagnosed with short tandem repeat analysis: Case report and review of the literature. Int J Gynecol Cancer. 17:254–258. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Kobayashi Y, Shimizu T, Naoe H, Ueki A, Ishizawa J, Chiyoda T, Onishi N, Sugihara E, Nagano O, Banno K, et al: Establishment of a choriocarcinoma model from immortalized normal extravillous trophoblast cells transduced with HRASV12. Am J Pathol. 179:1471–1482. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Wu S, Liu B, Zhang Q, Liu J, Zhou W, Wang C, Li M, Bao S and Zhu R: Dihydromyricetin reduced Bcl-2 expression via p53 in human hepatoma HepG2 cells. PLoS One. 8:e768862013. View Article : Google Scholar : PubMed/NCBI

6 

Ye J, Guan Y, Zeng S and Liu D: Ampelopsin prevents apoptosis induced by H2O2 in MT-4 lymphocytes. Planta Med. 74:252–257. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Wu Y, Bai J, Zhong K, Huang Y and Gao H: A dual antibacterial mechanism involved in membrane disruption and DNA binding of 2R,3R-dihydromyricetin from pine needles of Cedrus deodara against Staphylococcus aureus. Food Chem. 218:463–470. 2017. View Article : Google Scholar : PubMed/NCBI

8 

Xu B, Huang S, Wang C, Zhang H, Fang S and Zhang Y: Anti-inflammatory effects of dihydromyricetin in a mouse model of asthma. Mol Med Rep. 15:3674–3680. 2017. View Article : Google Scholar : PubMed/NCBI

9 

Liu B, Zhou W, Chen X, Xu F, Chen Y, Liu J, Zhang Q, Bao S, Chen N, Li M and Zhu R: Dihydromyricetin induces mouse hepatoma Hepal-6 cell apoptosis via the transforming growth factor-β pathway. Mol Med Rep. 11:1609–1614. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Wang JT, Jiao P, Zhou Y and Liu Q: Protective effect of dihydromyricetin against lipopolysaccharide-induced acute kidney injury in a rat model. Med Sci Monit. 22:454–459. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Fan TF, Wu TF, Bu LL, Ma SR, Li YC, Mao L, Sun ZJ and Zhang WF: Dihydromyricetin promotes autophagy and apoptosis through ROS-STAT3 signaling in head and neck squamous cell carcinoma. Oncotarget. 7:59691–59703. 2016. View Article : Google Scholar : PubMed/NCBI

12 

Zhang Z, Zhang H, Chen S, Xu Y, Yao A, Liao Q, Han L, Zou Z and Zhang X: Dihydromyricetin induces mitochondria-mediated apoptosis in HepG2 cells through down-regulation of the Akt/Bad pathway. Nutr Res. 38:27–33. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Ji FJ, Tian XF, Liu XW, Fu LB, Wu YY, Fang XD and Jin HY: Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells. Genet Mol Res. 14:15564–15571. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Kao SJ, Lee WJ, Chang JH, Chow JM, Chung CL, Hung WY and Chien MH: Suppression of reactive oxygen species-mediated ERK and JNK activation sensitizes dihydromyricetin-induced mitochondrial apoptosis in human non-small cell lung cancer. Environ Toxicol. 32:1426–1438. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Zeng S, Liu D, Ye Y, Wang L and Wang W: Anti-tumor effects of ampelopsin on human lung cancer GLC-82 implanted in nude mice. Zhong Yao Cai. 27:842–845. 2004.(In Chinese). PubMed/NCBI

16 

Luo GQ, Zeng S and Liu DY: Inhibitory effects of ampelopsin on angiogenesis. Zhong Yao Cai. 29:146–150. 2006.(In Chinese). PubMed/NCBI

17 

Zhou FZ and Zhang XF: Suppression of distant pulmonary metastasis of 4T1 mice breast carcinoma by dihydromyricetin administration. Chin J Clinicians(Electronic Ed). 8:1674–1678. 2014.(In Chinese).

18 

Jiang L, Zhang Q, Ren H, Ma S, Lu C, Liu B, Liu J, Liang J, Li M and Zhu R: Dihydromyricetin enhances the chemo-sensitivity of nedaplatin via regulation of the p53/Bcl-2 pathway in hepatocellular carcinoma cells. PLoS One. 10:e01249942015. View Article : Google Scholar : PubMed/NCBI

19 

Wong IL, Wang BC, Yuan J, Duan LX, Liu Z, Liu T, Li XM, Hu X, Zhang XY, Jiang T, et al: Potent and nontoxic chemosensitizer of P-Glycoprotein-mediated multidrug resistance in cancer: Synthesis and evaluation of methylated epigallocatechin, gallocatechin, and dihydromyricetin derivatives. J Med Chem. 58:4529–4549. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Su DL, Huang JH and Yao MJ: The acute toxicological evaluation of dihydromyricetin and its control effect for alcoholic hepatic injury. Hun Agricultural Sci. 90–93. 2009.(In Chinese).

21 

Biaglow JE and Miller RA: The thioredoxin reductase/thioredoxin system: Novel redox targets for cancer therapy. Cancer Biol Ther. 4:6–13. 2005. View Article : Google Scholar : PubMed/NCBI

22 

Kiraz Y, Adan A, Yandim Kartal M and Baran Y: Major apoptotic mechanisms and genes involved in apoptosis. Tumour Biol. 37:8471–8486. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Czabotar PE, Lessene G, Strasser A and Adams JM: Control of apoptosis by the BCL-2 protein family: Implications for physiology and therapy. Nat Rev Mol Cell Biol. 15:49–63. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Ghoneum M, Matsuura M, Braga M and Gollapudi S: S. cerevisiae induces apoptosis in human metastatic breast cancer cells by altering intracellular Ca2+ and the ratio of Bax and Bcl-2. Int J Oncol. 33:533–539. 2008.PubMed/NCBI

25 

Kluck RM, Bossy-Wetzel E, Green DR and Newmeyer DD: The release of cytochrome c from mitochondria: A primary site for Bcl-2 regulation of apoptosis. Science. 275:1132–1136. 1997. View Article : Google Scholar : PubMed/NCBI

26 

Bar-Am O, Weinreb O, Amit T and Youdim MB: Regulation of Bcl-2 family proteins, neurotrophic factors, and APP processing in the neurorescue activity of propargylamine. FASEB J. 19:1899–1901. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Prenek L, Boldizsár F, Kugyelka R, Ugor E, Berta G, Németh P and Berki T: The regulation of the mitochondrial apoptotic pathway by glucocorticoid receptor in collaboration with Bcl-2 family proteins in developing T cells. Apoptosis. 22:239–253. 2017. View Article : Google Scholar : PubMed/NCBI

28 

Porter AG and Jänicke RU: Emerging roles of caspase-3 in apoptosis. Cell Death Differ. 6:99–104. 1999. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zuo Y, Xu Q, Lu Y, Sun D, Wang K, Lei Y, Liang X and Li Y: Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line. Oncol Lett 16: 4229-4234, 2018.
APA
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y. ... Li, Y. (2018). Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line. Oncology Letters, 16, 4229-4234. https://doi.org/10.3892/ol.2018.9220
MLA
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y., Liang, X., Li, Y."Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line". Oncology Letters 16.4 (2018): 4229-4234.
Chicago
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y., Liang, X., Li, Y."Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line". Oncology Letters 16, no. 4 (2018): 4229-4234. https://doi.org/10.3892/ol.2018.9220
Copy and paste a formatted citation
x
Spandidos Publications style
Zuo Y, Xu Q, Lu Y, Sun D, Wang K, Lei Y, Liang X and Li Y: Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line. Oncol Lett 16: 4229-4234, 2018.
APA
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y. ... Li, Y. (2018). Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line. Oncology Letters, 16, 4229-4234. https://doi.org/10.3892/ol.2018.9220
MLA
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y., Liang, X., Li, Y."Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line". Oncology Letters 16.4 (2018): 4229-4234.
Chicago
Zuo, Y., Xu, Q., Lu, Y., Sun, D., Wang, K., Lei, Y., Liang, X., Li, Y."Dihydromyricetin induces apoptosis in a human choriocarcinoma cell line". Oncology Letters 16, no. 4 (2018): 4229-4234. https://doi.org/10.3892/ol.2018.9220
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