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Article Open Access

Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin

  • Authors:
    • Yingzhao Liu
    • Yan Feng
    • Hongmei Liu
    • Jianyong Wu
    • Yong Tang
    • Qi Wang
  • View Affiliations / Copyright

    Affiliations: Research Department, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4253-4262
    |
    Published online on: July 27, 2018
       https://doi.org/10.3892/ol.2018.9223
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Abstract

The aim of the present study was to perform a rapid evaluation of the efficiency of commonly used platinum‑based chemotherapy regimens for patients with ovarian cancer with extensive metastases using an in vitro method combined with culturing primary cells and real‑time monitoring, and to further explore the enhanced effect of metformin on susceptibility of ovarian cancer cells to platinum‑based chemotherapy. The primary omental metastatic (OM) cells were isolated from the omentum metastasis of a surgical patient with stage IIIc ovarian carcinoma. Drug sensitivity was evaluated using the xCELLigence system, and screening of the most effective platinum chemotherapy was performed through analysis of cell susceptibility to cisplatin, carboplatin, nedaplatin and paclitaxel or docetaxel alone or in combination. At the same time, this system was used to determine whether metformin was able to increase the sensitivity of cancer cells to platinum chemotherapy. The results revealed that nedaplatin exhibited the most marked cytotoxic effect on the OM cells, followed by those of carboplatin and cisplatin. The addition of docetaxel enhanced the cytotoxic effect, and the combination of platinum and paclitaxel also enhanced the effect. Metformin rapidly increased the sensitivity of cells to platinum‑based chemotherapy, and this effect was dose‑dependent. The sensitivity of OM cells to different platinum‑based regimens was varied. The effect of metformin on chemotherapeutic sensitization of cancer cells is clear in vitro, and the real‑time cell analyzer assay has the potential to assist in determining individualized drug regimens for patients with metastatic ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Feng Y, Liu H, Wu J, Tang Y and Wang Q: Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin. Oncol Lett 16: 4253-4262, 2018.
APA
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., & Wang, Q. (2018). Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin. Oncology Letters, 16, 4253-4262. https://doi.org/10.3892/ol.2018.9223
MLA
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., Wang, Q."Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin". Oncology Letters 16.4 (2018): 4253-4262.
Chicago
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., Wang, Q."Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin". Oncology Letters 16, no. 4 (2018): 4253-4262. https://doi.org/10.3892/ol.2018.9223
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Feng Y, Liu H, Wu J, Tang Y and Wang Q: Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin. Oncol Lett 16: 4253-4262, 2018.
APA
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., & Wang, Q. (2018). Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin. Oncology Letters, 16, 4253-4262. https://doi.org/10.3892/ol.2018.9223
MLA
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., Wang, Q."Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin". Oncology Letters 16.4 (2018): 4253-4262.
Chicago
Liu, Y., Feng, Y., Liu, H., Wu, J., Tang, Y., Wang, Q."Real‑time assessment of platinum sensitivity of primary culture from a patient with ovarian cancer with extensive metastasis and the platinum sensitivity enhancing effect by metformin". Oncology Letters 16, no. 4 (2018): 4253-4262. https://doi.org/10.3892/ol.2018.9223
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