Open Access

MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene

  • Authors:
    • Binbin Zhang
    • Tao Fu
    • Lun Zhang
  • View Affiliations

  • Published online on: August 13, 2018     https://doi.org/10.3892/ol.2018.9302
  • Pages: 5075-5083
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Human laryngeal squamous cell carcinoma (LSCC) is a malignant cancer type. Epithelial‑mesenchymal transition marker Snail family transcriptional repressor 1 (SNAI1) is associated with the occurrence, development, invasion and metastasis of numerous tumor types, such as lung, liver and ovarian cancer. Previous studies have indicated that microRNA‑153 (miR‑153) may serve as a novel tumor suppressor, which is involved in tumor metastasis; however, the role and clinical significance of miR‑153 in LSCC are not fully understood. The aim of the present study was to determine the role of miR‑153 in the growth and aggressiveness of LSCC cells. Bioinformatics prediction method, western blot analysis, Matrigel invasion assay and immunofluorescence were used to analyze whether SNAI1 can be regulated and controlled by miR‑153 in LSCC cells. An inverse association between miR‑153 and SNAI1 was observed in LSCC tissues. It was demonstrated that SNAI1 is a direct target of miR‑153 in LSCC. In addition, the results indicated that miR‑153 knockdown inhibited PCI‑13 cell migration and invasion by targeting SNAI1, which may be a potential marker that can reflect the degree of malignancy in patients with LSCC. Furthermore, miR‑153 knockdown decreased Twist family BHLH transcription factor 1 and metastasis‑associated 1 family member 3 expression in LSCC cells. In conclusion, these data indicated that miR‑153 regulates LSCC migration via the targeting of SNAI1 gene, which may be a potential predictor for patients with LSCC.
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October-2018
Volume 16 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang B, Fu T and Zhang L: MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene. Oncol Lett 16: 5075-5083, 2018
APA
Zhang, B., Fu, T., & Zhang, L. (2018). MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene. Oncology Letters, 16, 5075-5083. https://doi.org/10.3892/ol.2018.9302
MLA
Zhang, B., Fu, T., Zhang, L."MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene". Oncology Letters 16.4 (2018): 5075-5083.
Chicago
Zhang, B., Fu, T., Zhang, L."MicroRNA‑153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene". Oncology Letters 16, no. 4 (2018): 5075-5083. https://doi.org/10.3892/ol.2018.9302