Antitumorigenic effect of damnacanthal on melanoma cell viability through p53 and NF‑κB/caspase‑3 signaling pathways

  • Authors:
    • Xin Zhang
    • Ping Fang
    • Zigang Zhao
    • Xiangyu Ding
    • Fang Xie
    • Yilin Wang
    • Chengxin Li
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  • Published online on: September 3, 2018     https://doi.org/10.3892/ol.2018.9379
  • Pages: 6039-6044
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Abstract

Melanoma is highly malignant, particularly prone to metastasizing to the skin. The incidence of melanoma varies markedly between countries, and is relatively low in China. The aim of the present study was to investigate the antitumorigenic effect of damnacanthal on melanoma cells, and its molecular mechanism. MUM‑2B cells were treated with 0‑20 µM damnacanthal for 12, 24 and 48 h. In vitro, it was demonstrated that damnacanthal inhibited proliferation and promoted apoptosis of melanoma cells in a dose‑ and time‑dependent manner. Damnacanthal treatment increased caspase‑3/8 and 9 activity, and promoted B‑cell lymphoma 2‑associated X protein, tumor protein p53 (p53) and p21 protein expression levels in melanoma cells. Damnacanthal treatment also resulted in downregulated nuclear factor‑κB (NF‑κB), cyclin D and cyclin E protein expression in melanoma cells. In conclusion, the results of the present study demonstrated that the antitumorigenic activity of damnacanthal on melanoma cells is executed via the p53/p21 and NF‑κB/cyclin/ caspase‑3 signaling pathways.
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November 2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Zhang, X., Fang, P., Zhao, Z., Ding, X., Xie, F., Wang, Y., & Li, C. (2018). Antitumorigenic effect of damnacanthal on melanoma cell viability through p53 and NF‑κB/caspase‑3 signaling pathways. Oncology Letters, 16, 6039-6044. https://doi.org/10.3892/ol.2018.9379
MLA
Zhang, X., Fang, P., Zhao, Z., Ding, X., Xie, F., Wang, Y., Li, C."Antitumorigenic effect of damnacanthal on melanoma cell viability through p53 and NF‑κB/caspase‑3 signaling pathways". Oncology Letters 16.5 (2018): 6039-6044.
Chicago
Zhang, X., Fang, P., Zhao, Z., Ding, X., Xie, F., Wang, Y., Li, C."Antitumorigenic effect of damnacanthal on melanoma cell viability through p53 and NF‑κB/caspase‑3 signaling pathways". Oncology Letters 16, no. 5 (2018): 6039-6044. https://doi.org/10.3892/ol.2018.9379