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Article

Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer

  • Authors:
    • Jopi J.W. Mikkonen
    • Surya P. Singh
    • Ramin Akhi
    • Tuula Salo
    • Reijo Lappalainen
    • Wilfredo A. González‑Arriagada
    • Márcio Ajudarte Lopes
    • Arja M. Kullaa
    • Sami Myllymaa
  • View Affiliations / Copyright

    Affiliations: SIB Labs, University of Eastern Finland, FI‑70211 Kuopio, Finland, Institute of Dentistry, University of Eastern Finland, FI‑70211 Kuopio, Finland, Research Unit of Oral Health Sciences, University of Oulu, FI‑90014 Oulu, Finland, Cancer and Translational Medicine Research Unit, University of Oulu, FI‑90014 Oulu, Finland, Oral Pathology and Diagnosis, School of Dentistry, Universidad de Valparaiso, Valparaiso, Región de Valparaíso 2360004, Chile, Department of Oral Diagnosis, School of Dentistry, State University of Campinas, Sao Paulo CEP 13414‑018, Brazil
  • Pages: 6795-6800
    |
    Published online on: September 7, 2018
       https://doi.org/10.3892/ol.2018.9419
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Abstract

The analysis of the salivary metabolomic profile may offer an early phase approach to assess the changes associated with a wide range of diseases including head and neck cancer. The aim of the present study was to investigate the potential of nuclear magnetic resonance (NMR) spectroscopy for detecting the salivary metabolic changes associated with head and neck squamous cell carcinoma (HNSCC). Unstimulated whole‑mouth saliva samples collected from HNSCC patients (primary tumour was located either in the larynx or in the oral cavity) and healthy controls were analysed by 1H‑NMR spectroscopy. Reliably identified salivary metabolites were quantified and the determined concentration values were compared group‑wise using a Mann‑Whitney U‑test. Multivariate discrimination function analysis (DFA) was conducted to identify such a combination of metabolites, when considered together, that gives maximum discrimination between the groups. HNSCC patients exhibited significantly increased concentrations of 1,2‑propanediol (P=0.032) and fucose (P=0.003), while proline levels were significantly decreased (P=0.043). In the DFA model, the most powerful discrimination was achieved when fucose, glycine, methanol and proline were considered as combined biomarkers, resulting in a correct classification rate of 92.1%, sensitivity of 87.5% and specificity of 93.3%. To conclude, NMR spectrometric analysis was revealed to be a feasible approach to study the metabolome of saliva that is sensitive to metabolic changes in HNSCC and straightforward to collect in a non‑invasive manner. Salivary fucose was of particular interest and therefore, controlled longitudinal studies are required to assess its clinical relevance as a diagnostic biomarker in HNSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Mikkonen JJ, Singh SP, Akhi R, Salo T, Lappalainen R, González‑Arriagada WA, Ajudarte Lopes M, Kullaa AM and Myllymaa S: Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer. Oncol Lett 16: 6795-6800, 2018.
APA
Mikkonen, J.J., Singh, S.P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W.A. ... Myllymaa, S. (2018). Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer. Oncology Letters, 16, 6795-6800. https://doi.org/10.3892/ol.2018.9419
MLA
Mikkonen, J. J., Singh, S. P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W. A., Ajudarte Lopes, M., Kullaa, A. M., Myllymaa, S."Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer". Oncology Letters 16.5 (2018): 6795-6800.
Chicago
Mikkonen, J. J., Singh, S. P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W. A., Ajudarte Lopes, M., Kullaa, A. M., Myllymaa, S."Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer". Oncology Letters 16, no. 5 (2018): 6795-6800. https://doi.org/10.3892/ol.2018.9419
Copy and paste a formatted citation
x
Spandidos Publications style
Mikkonen JJ, Singh SP, Akhi R, Salo T, Lappalainen R, González‑Arriagada WA, Ajudarte Lopes M, Kullaa AM and Myllymaa S: Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer. Oncol Lett 16: 6795-6800, 2018.
APA
Mikkonen, J.J., Singh, S.P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W.A. ... Myllymaa, S. (2018). Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer. Oncology Letters, 16, 6795-6800. https://doi.org/10.3892/ol.2018.9419
MLA
Mikkonen, J. J., Singh, S. P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W. A., Ajudarte Lopes, M., Kullaa, A. M., Myllymaa, S."Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer". Oncology Letters 16.5 (2018): 6795-6800.
Chicago
Mikkonen, J. J., Singh, S. P., Akhi, R., Salo, T., Lappalainen, R., González‑Arriagada, W. A., Ajudarte Lopes, M., Kullaa, A. M., Myllymaa, S."Potential role of nuclear magnetic resonance spectroscopy to identify salivary metabolite alterations in patients with head and neck cancer". Oncology Letters 16, no. 5 (2018): 6795-6800. https://doi.org/10.3892/ol.2018.9419
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