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Article

Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling

  • Authors:
    • Weijuan Yan
    • Huijie Yu
    • Wei Li
    • Fengsheng Li
    • Sinian Wang
    • Nan Yu
    • Qisheng Jiang
  • View Affiliations / Copyright

    Affiliations: Laboratory of Nuclear and Radiation Damage, The General Hospital of The Second Artillery Corps of Chinese PLA, Beijing 100088, P.R. China
  • Pages: 6801-6807
    |
    Published online on: September 14, 2018
       https://doi.org/10.3892/ol.2018.9437
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Abstract

Polo‑like kinase (Plk)1 contributes to the development of human cancer via multiple mechanisms, such as promoting the migration of cancer cells. However, the mechanistic basis for the regulation of cell migration by Plk1 remains unknown. To address this question, the present study investigated the effect of Plk1 inhibition on the migration of human lung adenocarcinoma epithelial A549 cells and the molecular factors involved. A549 cells were treated with the Plk1 inhibitor, BI2536, and cell migration was evaluated with the wound‑healing assay. The expression of matrix metallopeptidase (MMP)2, vascular endothelial growth factor (VEGF)A, total and phosphorylated signal transducer and activator of transcription (STAT)3 was assessed by western blotting and reverse transcription‑polymerase chain reaction following Plk1 knockdown and/or STAT3 overexpression. The interaction between Plk1 and STAT3 was evaluated by co‑immunoprecipitation. The levels of MMP2 and VEGFA were decreased by treatment with Plk1 inhibitor. The phosphorylation of STAT3, which acts upstream of MMP2 and VEGFA, was also decreased by Plk1 knockdown, an effect that was abrogated by STAT3 overexpression. In addition, Plk1 was detected to bind with STAT3 either directly or as part of a complex by co‑immunoprecipitation experiments. These results indicated that Plk1 may promote the migration of A549 cells via regulation of STAT3 signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Yan W, Yu H, Li W, Li F, Wang S, Yu N and Jiang Q: Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling. Oncol Lett 16: 6801-6807, 2018.
APA
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., & Jiang, Q. (2018). Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling. Oncology Letters, 16, 6801-6807. https://doi.org/10.3892/ol.2018.9437
MLA
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., Jiang, Q."Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling". Oncology Letters 16.5 (2018): 6801-6807.
Chicago
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., Jiang, Q."Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling". Oncology Letters 16, no. 5 (2018): 6801-6807. https://doi.org/10.3892/ol.2018.9437
Copy and paste a formatted citation
x
Spandidos Publications style
Yan W, Yu H, Li W, Li F, Wang S, Yu N and Jiang Q: Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling. Oncol Lett 16: 6801-6807, 2018.
APA
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., & Jiang, Q. (2018). Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling. Oncology Letters, 16, 6801-6807. https://doi.org/10.3892/ol.2018.9437
MLA
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., Jiang, Q."Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling". Oncology Letters 16.5 (2018): 6801-6807.
Chicago
Yan, W., Yu, H., Li, W., Li, F., Wang, S., Yu, N., Jiang, Q."Plk1 promotes the migration of human lung adenocarcinoma epithelial cells via STAT3 signaling". Oncology Letters 16, no. 5 (2018): 6801-6807. https://doi.org/10.3892/ol.2018.9437
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