MicroRNA‑661 expression is upregulated in pancreatic ductal adenocarcinoma and promotes cell proliferation

Lv,Feifei; Zheng,Kehong; Yu,Jinlong; Huang,Zonghai

doi:10.3892/ol.2018.9454

MicroRNA‑661 expression is upregulated in pancreatic ductal adenocarcinoma and promotes cell proliferation

Authors:
- Feifei Lv
- Kehong Zheng
- Jinlong Yu
- Zonghai Huang
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Affiliations: Department of General Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China
Published online on: September 18, 2018 https://doi.org/10.3892/ol.2018.9454
Pages: 6293-6298
Copyright: © Lv et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Deregulation of microRNA (miRNA/miR) expression has been implicated in the development of pancreatic ductal adenocarcinoma (PDAC). However, the role of miR‑661 in PDAC remains unknown. In the present study, it was revealed that miR‑661 expression was significantly upregulated in PDAC tissues compared with that in adjacent normal tissues by using reverse transcription‑quantitative polymerase chain reaction assays. Higher miR‑661 expression revealed a positive association with lymph node metastasis, an advanced T stage and a poor prognosis in patients with PDAC. Furthermore, ectopic expression of miR‑661 significantly promoted the cell proliferation ability in PDAC cell lines, and simultaneously promoted Wnt signaling pathway‑related protein expression of β‑catenin, transcription factor 4 and cyclin D1 in vitro. However, the downregulation of miR‑661 revealed reverse effects. Thus, the results of the present study indicated that miR‑661 may function as a prognostic marker and provide insight for pancreatic cancer treatment.

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