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Article Open Access

MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway

  • Authors:
    • Yinlian Cha
    • Ying He
    • Kaobin Ouyang
    • Hailin Xiong
    • Jun Li
    • Xia Yuan
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, Huizhou Municipal Central Hospital of Guangdong Province, Huizhou, Guangdong 516000, P.R. China
    Copyright: © Cha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 6369-6376
    |
    Published online on: September 21, 2018
       https://doi.org/10.3892/ol.2018.9480
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Abstract

MicroRNAs have been suggested as potential regulators in gastric cancer (GC) development through affecting the expression of their target genes. Previous studies have demonstrated that miR‑140‑5p is downregulated in GC. However, the underlying functional role of miR‑140‑5p in GC remains largely unknown. The present study revealed that miR‑140‑5p expression was significantly decreased in 60 GC tissues, compared with corresponding adjacent non‑tumor tissues. A lower miR‑140‑5p expression was significantly associated with lymph node metastasis and an advanced Tumor‑Node‑Metastasis stage in patients with GC. Furthermore, patients with a lower miR‑140‑5p expression exhibited shorter disease‑free survival and overall survival times. Gain‑ and loss‑of‑function assays revealed that increased miR‑140‑5p expression significantly inhibited GC cell proliferation and invasion ability, as well as the Wnt/β‑catenin signaling pathway by decreasing WNT1 and β‑catenin expression. However, decreasing miR‑140‑5p expression had the opposite effects. Bioinformatics methods and dual‑luciferase reporter assays revealed that WNT1 was a direct target of miR‑140‑5p. miR‑140‑5p suppressed cell proliferation and invasion by regulating WNT1 expression. Therefore, the results of the present study demonstrated that miR‑140‑5p may serve as a potential prognostic marker and therapeutic target in patients with GC.
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1 

Leja M, You W, Camargo MC and Saito H: Implementation of gastric cancer screening-the global experience. Best Pract Res Clin Gastroenterol. 28:1093–1106. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Carcas LP: Gastric cancer review. J Carcinog. 13:142014. View Article : Google Scholar : PubMed/NCBI

3 

Rao M, Zhu Y, Cong X and Li Q: Knockdown of CREB1 inhibits tumor growth of human gastric cancer in vitro and in vivo. Oncol Rep. 37:3361–3368. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Xie M, Dart DA, Guo T, Xing XF, Cheng XJ, Du H, Jiang WG, Wen XZ and Ji JF: MicroRNA-1 acts as a tumor suppressor microRNA by inhibiting angiogenesis-related growth factors in human gastric cancer. Gastric Cancer. 21:41–54. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Jing P, Sa N, Liu X, Liu X and Xu W: MicroR-140-5p suppresses tumor cell migration and invasion by targeting ADAM10-mediated Notch1 signaling pathway in hypopharyngeal squamous cell carcinoma. Exp Mol Pathol. 100:132–138. 2016. View Article : Google Scholar : PubMed/NCBI

7 

Wei R, Cao G, Deng Z, Su J and Cai L: miR-140-5p attenuates chemotherapeutic drug-induced cell death by regulating autophagy through inositol 1,4,5-trisphosphate kinase 2 (IP3k2) in human osteosarcoma cells. Biosci Rep. 36:e003922016. View Article : Google Scholar : PubMed/NCBI

8 

Fang Z, Yin S, Sun R, Zhang S, Fu M, Wu Y, Zhang T, Khaliq J and Li Y: miR-140-5p suppresses the proliferation, migration and invasion of gastric cancer by regulating YES1. Mol Cancer. 16:1392017. View Article : Google Scholar : PubMed/NCBI

9 

Novák J and Fabian P: Comments on the TNM classification of malignant tumours-7th edition. Klin Onkol. 24:149–150. 2011.(In Czech). PubMed/NCBI

10 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Zhang T, Wu Y, Fang Z, Yan Q, Zhang S, Sun R, Khaliq J and Li Y: Low expression of RBMS3 and SFRP1 are associated with poor prognosis in patients with gastric cancer. Am J Cancer Res. 6:2679–2689. 2016.PubMed/NCBI

12 

Wang J, Chen X, Su L, Li P, Cai Q, Liu B, Wu W and Zhu Z: MicroRNA-126 inhibits cell proliferation in gastric cancer by targeting LAT-1. Biomed Pharmacother. 72:66–73. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Xiang XJ, Deng J, Liu YW, Wan LY, Feng M, Chen J and Xiong JP: MiR-1271 inhibits cell proliferation, invasion and EMT in gastric cancer by targeting FOXQ1. Cell Physiol Biochem. 36:1382–1394. 2015. View Article : Google Scholar : PubMed/NCBI

14 

Yang H, Fang F, Chang R and Yang L: MicroRNA-140-5p suppresses tumor growth and metastasis by targeting transforming growth factor β receptor 1 and fibroblast growth factor 9 in hepatocellular carcinoma. Hepatology. 58:205–217. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Li W and He F: Monocyte to macrophage differentiation-associated (MMD) targeted by miR-140-5p regulates tumor growth in non-small cell lung cancer. Biochem Biophys Res Commun. 450:844–850. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Zhai H, Fesler A, Ba Y, Wu S and Ju J: Inhibition of colorectal cancer stem cell survival and invasive potential by hsa-miR-140-5p mediated suppression of Smad2 and autophagy. Oncotarget. 6:19735–19746. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Zhang W, Zou C, Pan L, Xu Y, Qi W, Ma G, Hou Y and Jiang P: MicroRNA-140-5p inhibits the progression of colorectal cancer by targeting VEGFA. Cell Physiol Biochem. 37:1123–1133. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Yu Y, Yan W, Liu X, Jia Y, Cao B, Yu Y, Lv Y, Brock MV, Herman JG, Licchesi J, et al: DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling. Am J Cancer Res. 4:710–724. 2014.PubMed/NCBI

19 

Nunez F, Bravo S, Cruzat F, Montecino M and De Ferrari GV: Wnt/β-catenin signaling enhances cyclooxygenase-2 (COX2) transcriptional activity in gastric cancer cells. PLoS One. 6:e185622011. View Article : Google Scholar : PubMed/NCBI

20 

Yue H, Tang B, Zhao Y, Niu Y, Yin P, Yang W, Zhang Z and Yu P: MIR-519d suppresses the gastric cancer epithelial-mesenchymal transition via Twist1 and inhibits Wnt/β-catenin signaling pathway. Am J Transl Res. 9:3654–3664. 2017.PubMed/NCBI

21 

Wu F, Li J, Guo N, Wang XH and Liao YQ: MiRNA-27a promotes the proliferation and invasion of human gastric cancer MGC803 cells by targeting SFRP1 via Wnt/β-catenin signaling pathway. Am J Cancer Res. 7:405–416. 2017.PubMed/NCBI

22 

Peng Y, Zhang X, Ma Q, Yan R, Qin Y, Zhao Y, Cheng Y, Yang M, Wang Q, Feng X, et al: MiRNA-194 activates the Wnt/β-catenin signaling pathway in gastric cancer by targeting the negative Wnt regulator, SUFU. Cancer Lett. 385:117–127. 2017. View Article : Google Scholar : PubMed/NCBI

23 

Shan Y, Ying R, Jia Z, Kong W, Wu Y, Zheng S and Jin H: LINC00052 promotes gastric cancer cell proliferation and metastasis via activating Wnt/β-catenin signaling pathway. Oncol Res. 25:1589–1599. 2017. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cha Y, He Y, Ouyang K, Xiong H, Li J and Yuan X: MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncol Lett 16: 6369-6376, 2018.
APA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., & Yuan, X. (2018). MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncology Letters, 16, 6369-6376. https://doi.org/10.3892/ol.2018.9480
MLA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16.5 (2018): 6369-6376.
Chicago
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16, no. 5 (2018): 6369-6376. https://doi.org/10.3892/ol.2018.9480
Copy and paste a formatted citation
x
Spandidos Publications style
Cha Y, He Y, Ouyang K, Xiong H, Li J and Yuan X: MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncol Lett 16: 6369-6376, 2018.
APA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., & Yuan, X. (2018). MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncology Letters, 16, 6369-6376. https://doi.org/10.3892/ol.2018.9480
MLA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16.5 (2018): 6369-6376.
Chicago
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16, no. 5 (2018): 6369-6376. https://doi.org/10.3892/ol.2018.9480
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