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MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway

  • Authors:
    • Yinlian Cha
    • Ying He
    • Kaobin Ouyang
    • Hailin Xiong
    • Jun Li
    • Xia Yuan

  • View Affiliations

  • Published online on: September 21, 2018     https://doi.org/10.3892/ol.2018.9480
  • Pages: 6369-6376

  • Copyright: © Cha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

MicroRNAs have been suggested as potential regulators in gastric cancer (GC) development through affecting the expression of their target genes. Previous studies have demonstrated that miR‑140‑5p is downregulated in GC. However, the underlying functional role of miR‑140‑5p in GC remains largely unknown. The present study revealed that miR‑140‑5p expression was significantly decreased in 60 GC tissues, compared with corresponding adjacent non‑tumor tissues. A lower miR‑140‑5p expression was significantly associated with lymph node metastasis and an advanced Tumor‑Node‑Metastasis stage in patients with GC. Furthermore, patients with a lower miR‑140‑5p expression exhibited shorter disease‑free survival and overall survival times. Gain‑ and loss‑of‑function assays revealed that increased miR‑140‑5p expression significantly inhibited GC cell proliferation and invasion ability, as well as the Wnt/β‑catenin signaling pathway by decreasing WNT1 and β‑catenin expression. However, decreasing miR‑140‑5p expression had the opposite effects. Bioinformatics methods and dual‑luciferase reporter assays revealed that WNT1 was a direct target of miR‑140‑5p. miR‑140‑5p suppressed cell proliferation and invasion by regulating WNT1 expression. Therefore, the results of the present study demonstrated that miR‑140‑5p may serve as a potential prognostic marker and therapeutic target in patients with GC.
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November-2018
Volume 16 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Cha Y, He Y, Ouyang K, Xiong H, Li J and Yuan X: MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncol Lett 16: 6369-6376, 2018
APA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., & Yuan, X. (2018). MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway. Oncology Letters, 16, 6369-6376. https://doi.org/10.3892/ol.2018.9480
MLA
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16.5 (2018): 6369-6376.
Chicago
Cha, Y., He, Y., Ouyang, K., Xiong, H., Li, J., Yuan, X."MicroRNA‑140‑5p suppresses cell proliferation and invasion in gastric cancer by targeting WNT1 in the WNT/β‑catenin signaling pathway". Oncology Letters 16, no. 5 (2018): 6369-6376. https://doi.org/10.3892/ol.2018.9480